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Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

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Related in: MedlinePlus

Western blotting analysis.The anti-ICAM-1 MoAb was used for the detection. a: standard positive control loaded at 8000 pg; b: plasma sample loaded at 10000 pg, accordingly to ELISA detection; c: medium negative control; d: mature oocyte supernatant loaded at 35 pg accordingly to ELISA detection; e: immature oocyte supernatant loaded at 100 pg accordingly to ELISA detection; M: protein ladder.
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pone-0003970-g003: Western blotting analysis.The anti-ICAM-1 MoAb was used for the detection. a: standard positive control loaded at 8000 pg; b: plasma sample loaded at 10000 pg, accordingly to ELISA detection; c: medium negative control; d: mature oocyte supernatant loaded at 35 pg accordingly to ELISA detection; e: immature oocyte supernatant loaded at 100 pg accordingly to ELISA detection; M: protein ladder.

Mentions: The presence of sICAM-1 molecules in oocytes culture supernatants was also analyzed by western blotting. The results obtained are shown in Figure 3. Standard positive ICAM-1 controls are shown in lanes “a” and “b”. As clearly evident, sICAM-1 is detectable both in mature (lane “d”) and immature (lane “e”) oocyte supernatants. In addition, sICAM-1 is present in mature oocytes in lower quantities in respect to immature oocytes, fully in agreement with the Bio-Plex data shown in Figure 3. These data were fully in agreement with ELISA assays (data not shown).


Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

Western blotting analysis.The anti-ICAM-1 MoAb was used for the detection. a: standard positive control loaded at 8000 pg; b: plasma sample loaded at 10000 pg, accordingly to ELISA detection; c: medium negative control; d: mature oocyte supernatant loaded at 35 pg accordingly to ELISA detection; e: immature oocyte supernatant loaded at 100 pg accordingly to ELISA detection; M: protein ladder.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2599884&req=5

pone-0003970-g003: Western blotting analysis.The anti-ICAM-1 MoAb was used for the detection. a: standard positive control loaded at 8000 pg; b: plasma sample loaded at 10000 pg, accordingly to ELISA detection; c: medium negative control; d: mature oocyte supernatant loaded at 35 pg accordingly to ELISA detection; e: immature oocyte supernatant loaded at 100 pg accordingly to ELISA detection; M: protein ladder.
Mentions: The presence of sICAM-1 molecules in oocytes culture supernatants was also analyzed by western blotting. The results obtained are shown in Figure 3. Standard positive ICAM-1 controls are shown in lanes “a” and “b”. As clearly evident, sICAM-1 is detectable both in mature (lane “d”) and immature (lane “e”) oocyte supernatants. In addition, sICAM-1 is present in mature oocytes in lower quantities in respect to immature oocytes, fully in agreement with the Bio-Plex data shown in Figure 3. These data were fully in agreement with ELISA assays (data not shown).

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

Show MeSH
Related in: MedlinePlus