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Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

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Related in: MedlinePlus

sICAM-1 release in immature oocytes (black box) compared to mature (white box) oocytes and to in vitro fertilized embryo (grey box).Oocytes were individually cultured in a 4-well culture dish as reported in the methods section. Following the maturation period 250 µl of supernatants were collected from each culture system and stored at −20°C until being tested for the presence released proteins. Mature and immature oocytes were identified, one by one, evaluating the presence or absence of the first polar body. In vitro fertilized embryos were individually cultured in 4-well culture dishes and 250 µl of supernatants collected from each embryo culture and stored at −20°C until being tested for the presence of released proteins. * Student t Test.
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pone-0003970-g002: sICAM-1 release in immature oocytes (black box) compared to mature (white box) oocytes and to in vitro fertilized embryo (grey box).Oocytes were individually cultured in a 4-well culture dish as reported in the methods section. Following the maturation period 250 µl of supernatants were collected from each culture system and stored at −20°C until being tested for the presence released proteins. Mature and immature oocytes were identified, one by one, evaluating the presence or absence of the first polar body. In vitro fertilized embryos were individually cultured in 4-well culture dishes and 250 µl of supernatants collected from each embryo culture and stored at −20°C until being tested for the presence of released proteins. * Student t Test.

Mentions: Figure 2 reports a sharp difference in sICAM-1 levels among immature and mature oocytes and fertilized embryos. The average sICAM-1 production by immature (n = 39) and mature (n = 73) oocytes was 6711.5±1502.4 and 2987±103.7 pg/ml/24 hours (mean±SD), respectively (Figure 2). This difference was very reproducible and statistically significant (Student t Test, p<0.0001). In addition, the levels of release of sICAM-1 levels by mature oocytes and in vitro fertilized embryos (n = 73), 1486.8±164.2 pg/ml/24 hours, were also found to be significantly different (Student t Test, p<0.0001) (Figure 2). Therefore, it appears that the release of sICAM-1 has a clear tendency to decrease from immature embryos, to mature embryos and to fertilized embryos.


Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

sICAM-1 release in immature oocytes (black box) compared to mature (white box) oocytes and to in vitro fertilized embryo (grey box).Oocytes were individually cultured in a 4-well culture dish as reported in the methods section. Following the maturation period 250 µl of supernatants were collected from each culture system and stored at −20°C until being tested for the presence released proteins. Mature and immature oocytes were identified, one by one, evaluating the presence or absence of the first polar body. In vitro fertilized embryos were individually cultured in 4-well culture dishes and 250 µl of supernatants collected from each embryo culture and stored at −20°C until being tested for the presence of released proteins. * Student t Test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2599884&req=5

pone-0003970-g002: sICAM-1 release in immature oocytes (black box) compared to mature (white box) oocytes and to in vitro fertilized embryo (grey box).Oocytes were individually cultured in a 4-well culture dish as reported in the methods section. Following the maturation period 250 µl of supernatants were collected from each culture system and stored at −20°C until being tested for the presence released proteins. Mature and immature oocytes were identified, one by one, evaluating the presence or absence of the first polar body. In vitro fertilized embryos were individually cultured in 4-well culture dishes and 250 µl of supernatants collected from each embryo culture and stored at −20°C until being tested for the presence of released proteins. * Student t Test.
Mentions: Figure 2 reports a sharp difference in sICAM-1 levels among immature and mature oocytes and fertilized embryos. The average sICAM-1 production by immature (n = 39) and mature (n = 73) oocytes was 6711.5±1502.4 and 2987±103.7 pg/ml/24 hours (mean±SD), respectively (Figure 2). This difference was very reproducible and statistically significant (Student t Test, p<0.0001). In addition, the levels of release of sICAM-1 levels by mature oocytes and in vitro fertilized embryos (n = 73), 1486.8±164.2 pg/ml/24 hours, were also found to be significantly different (Student t Test, p<0.0001) (Figure 2). Therefore, it appears that the release of sICAM-1 has a clear tendency to decrease from immature embryos, to mature embryos and to fertilized embryos.

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

Show MeSH
Related in: MedlinePlus