Limits...
Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

Show MeSH

Related in: MedlinePlus

ELISA and Bio-Plex standard curves (white circles and black square respectively) have been obtained with 50 µl of ICAM-1 standard reagent at the concentrations of 0.25, 0.5, 1, 2, 4, 8 ng/ml or 0.0002, 0.00081, 0.0032, 0.0129, 0.0516, 0.20638, 0.82522, 3.3 ng/ml as indicated.FI: fluorescence intensity values. OD 450 nm: optic density at 450 nm wavelength.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2599884&req=5

pone-0003970-g001: ELISA and Bio-Plex standard curves (white circles and black square respectively) have been obtained with 50 µl of ICAM-1 standard reagent at the concentrations of 0.25, 0.5, 1, 2, 4, 8 ng/ml or 0.0002, 0.00081, 0.0032, 0.0129, 0.0516, 0.20638, 0.82522, 3.3 ng/ml as indicated.FI: fluorescence intensity values. OD 450 nm: optic density at 450 nm wavelength.

Mentions: In Figure 1 representative analysis is shown demonstrating that levels of ICAM-1 standards are detectable following both ELISA and the Bio-Plex assay. As expected, however, the Bio-Plex assay is more sensitive than ELISA. This is of course important for analysis of single cells, including oocytes. Accordingly, Bio-Plex analysis was chosen for studies involving human oocytes and fertilized embryos.


Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

ELISA and Bio-Plex standard curves (white circles and black square respectively) have been obtained with 50 µl of ICAM-1 standard reagent at the concentrations of 0.25, 0.5, 1, 2, 4, 8 ng/ml or 0.0002, 0.00081, 0.0032, 0.0129, 0.0516, 0.20638, 0.82522, 3.3 ng/ml as indicated.FI: fluorescence intensity values. OD 450 nm: optic density at 450 nm wavelength.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2599884&req=5

pone-0003970-g001: ELISA and Bio-Plex standard curves (white circles and black square respectively) have been obtained with 50 µl of ICAM-1 standard reagent at the concentrations of 0.25, 0.5, 1, 2, 4, 8 ng/ml or 0.0002, 0.00081, 0.0032, 0.0129, 0.0516, 0.20638, 0.82522, 3.3 ng/ml as indicated.FI: fluorescence intensity values. OD 450 nm: optic density at 450 nm wavelength.
Mentions: In Figure 1 representative analysis is shown demonstrating that levels of ICAM-1 standards are detectable following both ELISA and the Bio-Plex assay. As expected, however, the Bio-Plex assay is more sensitive than ELISA. This is of course important for analysis of single cells, including oocytes. Accordingly, Bio-Plex analysis was chosen for studies involving human oocytes and fertilized embryos.

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

Show MeSH
Related in: MedlinePlus