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CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children.

Sharma TS, Hughes J, Murillo A, Riley J, Soares A, Little F, Mitchell CD, Hanekom WA - PLoS ONE (2008)

Bottom Line: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status.This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status. 51 HIV-infected children were enrolled.In contrast, we found no association between CD38 expression and these disease status markers.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Infectious Diseases, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America.

ABSTRACT

Background: Initiation and modification of antiretroviral therapy in HIV-infected children depend on viral load and CD4+ T-cell count. However, these surrogates have limitations, and complementary immunological markers to assess therapeutic response are needed. Our aim was to evaluate CD8+ T-cell expression of CD127 as a marker of disease status in HIV-infected children, based on adult data suggesting its usefulness. We hypothesized that CD127 expression on CD8+ T-cells is lower in children with more advanced disease.

Methods: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status. This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status.

Results: 51 HIV-infected children were enrolled. There was a strong positive correlation between CD127 expression on CD8+ T-cells and CD4+ T-cell count, and height and weight z-scores, and a strong negative correlation between CD127 expression and viral load. In contrast, we found no association between CD38 expression and these disease status markers.

Conclusions: CD8+ T-cell CD127 expression is significantly higher in children with better HIV disease control, and may have a role as an immunologic indicator of disease status. Longitudinal studies are needed to determine the utility of this marker as a potential indicator of HIV disease progression.

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A–B. Association between CD8+ T-cell expression of CD127 (A.) and of CD38 (B.) and plasma HIV viral load, in 48 HIV-infected children. The lower limit of detection of the plasma HIV assay was 400 copies/mL. A Spearman test was used to assess correlations. C.–D. Relationship of CD8+ T-cell expression of CD127(C) and of CD38 (D) with degree of virologic control, as categorized by the plasma HIV viral load. Mann Whitney test was used to assess differences between the groups.
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pone-0003986-g003: A–B. Association between CD8+ T-cell expression of CD127 (A.) and of CD38 (B.) and plasma HIV viral load, in 48 HIV-infected children. The lower limit of detection of the plasma HIV assay was 400 copies/mL. A Spearman test was used to assess correlations. C.–D. Relationship of CD8+ T-cell expression of CD127(C) and of CD38 (D) with degree of virologic control, as categorized by the plasma HIV viral load. Mann Whitney test was used to assess differences between the groups.

Mentions: There was a strong negative correlation between CD127 expression on CD8+ T-cells and HIV viral load (Figure 3a), but CD38 expression on these cells was not associated with viral load (Figure 3b). CD127 expression was highest in those children with excellent virologic control (Figure 3c) and lowest in those children with poor virologic control. No differential pattern could be seen for CD38 expression (Figure 3d).


CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children.

Sharma TS, Hughes J, Murillo A, Riley J, Soares A, Little F, Mitchell CD, Hanekom WA - PLoS ONE (2008)

A–B. Association between CD8+ T-cell expression of CD127 (A.) and of CD38 (B.) and plasma HIV viral load, in 48 HIV-infected children. The lower limit of detection of the plasma HIV assay was 400 copies/mL. A Spearman test was used to assess correlations. C.–D. Relationship of CD8+ T-cell expression of CD127(C) and of CD38 (D) with degree of virologic control, as categorized by the plasma HIV viral load. Mann Whitney test was used to assess differences between the groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2599882&req=5

pone-0003986-g003: A–B. Association between CD8+ T-cell expression of CD127 (A.) and of CD38 (B.) and plasma HIV viral load, in 48 HIV-infected children. The lower limit of detection of the plasma HIV assay was 400 copies/mL. A Spearman test was used to assess correlations. C.–D. Relationship of CD8+ T-cell expression of CD127(C) and of CD38 (D) with degree of virologic control, as categorized by the plasma HIV viral load. Mann Whitney test was used to assess differences between the groups.
Mentions: There was a strong negative correlation between CD127 expression on CD8+ T-cells and HIV viral load (Figure 3a), but CD38 expression on these cells was not associated with viral load (Figure 3b). CD127 expression was highest in those children with excellent virologic control (Figure 3c) and lowest in those children with poor virologic control. No differential pattern could be seen for CD38 expression (Figure 3d).

Bottom Line: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status.This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status. 51 HIV-infected children were enrolled.In contrast, we found no association between CD38 expression and these disease status markers.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Infectious Diseases, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America.

ABSTRACT

Background: Initiation and modification of antiretroviral therapy in HIV-infected children depend on viral load and CD4+ T-cell count. However, these surrogates have limitations, and complementary immunological markers to assess therapeutic response are needed. Our aim was to evaluate CD8+ T-cell expression of CD127 as a marker of disease status in HIV-infected children, based on adult data suggesting its usefulness. We hypothesized that CD127 expression on CD8+ T-cells is lower in children with more advanced disease.

Methods: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status. This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status.

Results: 51 HIV-infected children were enrolled. There was a strong positive correlation between CD127 expression on CD8+ T-cells and CD4+ T-cell count, and height and weight z-scores, and a strong negative correlation between CD127 expression and viral load. In contrast, we found no association between CD38 expression and these disease status markers.

Conclusions: CD8+ T-cell CD127 expression is significantly higher in children with better HIV disease control, and may have a role as an immunologic indicator of disease status. Longitudinal studies are needed to determine the utility of this marker as a potential indicator of HIV disease progression.

Show MeSH
Related in: MedlinePlus