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Comparative analysis of fatty acid desaturases in cyanobacterial genomes.

Chi X, Yang Q, Zhao F, Qin S, Yang Y, Shen J, Lin H - Comp. Funct. Genomics (2008)

Bottom Line: The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont.Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats.Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

ABSTRACT
Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N(2)-fixing cyanobacteria usually possess more types of fatty acid desaturases than that of unicellular species. The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont. Strain Gloeobacter violaceus PCC 7421 was isolated from calcareous rock and lacks thylakoid membranes. The types and amounts of desaturases of this strain are distinct to those of other cyanobacteria, reflecting the earliest divergence of it from the cyanobacterial line. Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats. Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

No MeSH data available.


Related in: MedlinePlus

Minimum-evolutiontree of membrane desaturases. About 330 positionsspanning the three histidine-boxes were employed.  Sequences from 37 sequenced cyanobacterial genomes are shown by their acronyms andaccession numbers (locus tags). Othersequences are shown by their accession numbers, labels, and strain names. Desaturase genes that have been functionally characterized are indicated on the tree by their labels.Bootstrap values from minimum-evolution analyses arelisted to the left of each node, with values more than 50 are shown.
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fig13: Minimum-evolutiontree of membrane desaturases. About 330 positionsspanning the three histidine-boxes were employed. Sequences from 37 sequenced cyanobacterial genomes are shown by their acronyms andaccession numbers (locus tags). Othersequences are shown by their accession numbers, labels, and strain names. Desaturase genes that have been functionally characterized are indicated on the tree by their labels.Bootstrap values from minimum-evolution analyses arelisted to the left of each node, with values more than 50 are shown.

Mentions: Toelucidate the phylogenetic relationships among different membrane desaturases,genes from cyanobacteria, eukaryotic algae, higher plants, fungi, invertebrates,and vertebrates were analyzed using neighbor-joining (NJ) and minimum-evolution(ME) methods. Observationof the tree revealed that all the desaturases fell into three distinctsubfamilies (Figures 12 and 13): Δ9 desaturase subfamily,Δ12/ω3 desaturases subfamily, and the front-end desaturases subfamily.


Comparative analysis of fatty acid desaturases in cyanobacterial genomes.

Chi X, Yang Q, Zhao F, Qin S, Yang Y, Shen J, Lin H - Comp. Funct. Genomics (2008)

Minimum-evolutiontree of membrane desaturases. About 330 positionsspanning the three histidine-boxes were employed.  Sequences from 37 sequenced cyanobacterial genomes are shown by their acronyms andaccession numbers (locus tags). Othersequences are shown by their accession numbers, labels, and strain names. Desaturase genes that have been functionally characterized are indicated on the tree by their labels.Bootstrap values from minimum-evolution analyses arelisted to the left of each node, with values more than 50 are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2593844&req=5

fig13: Minimum-evolutiontree of membrane desaturases. About 330 positionsspanning the three histidine-boxes were employed. Sequences from 37 sequenced cyanobacterial genomes are shown by their acronyms andaccession numbers (locus tags). Othersequences are shown by their accession numbers, labels, and strain names. Desaturase genes that have been functionally characterized are indicated on the tree by their labels.Bootstrap values from minimum-evolution analyses arelisted to the left of each node, with values more than 50 are shown.
Mentions: Toelucidate the phylogenetic relationships among different membrane desaturases,genes from cyanobacteria, eukaryotic algae, higher plants, fungi, invertebrates,and vertebrates were analyzed using neighbor-joining (NJ) and minimum-evolution(ME) methods. Observationof the tree revealed that all the desaturases fell into three distinctsubfamilies (Figures 12 and 13): Δ9 desaturase subfamily,Δ12/ω3 desaturases subfamily, and the front-end desaturases subfamily.

Bottom Line: The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont.Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats.Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

ABSTRACT
Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N(2)-fixing cyanobacteria usually possess more types of fatty acid desaturases than that of unicellular species. The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont. Strain Gloeobacter violaceus PCC 7421 was isolated from calcareous rock and lacks thylakoid membranes. The types and amounts of desaturases of this strain are distinct to those of other cyanobacteria, reflecting the earliest divergence of it from the cyanobacterial line. Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats. Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

No MeSH data available.


Related in: MedlinePlus