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Comparative analysis of fatty acid desaturases in cyanobacterial genomes.

Chi X, Yang Q, Zhao F, Qin S, Yang Y, Shen J, Lin H - Comp. Funct. Genomics (2008)

Bottom Line: The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont.Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats.Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

ABSTRACT
Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N(2)-fixing cyanobacteria usually possess more types of fatty acid desaturases than that of unicellular species. The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont. Strain Gloeobacter violaceus PCC 7421 was isolated from calcareous rock and lacks thylakoid membranes. The types and amounts of desaturases of this strain are distinct to those of other cyanobacteria, reflecting the earliest divergence of it from the cyanobacterial line. Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats. Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

No MeSH data available.


Related in: MedlinePlus

Comparison of the three conserved histidine-rich motifs of proteinsfrom cyanobacteria, eukaryotic algae, and higher plants, including Δ12 fattyacid desaturase, Δ15 fatty acid desaturase, β-carotene ketolase, β-carotenehydroxylase, hydrocarbon oxygenase, Δ12 fatty acid epoxygenase, Δ12 fatty acidacetylenase, Δ12 fatty acid conjugase, and Δ12 fatty acid hydroxylase. Theconserved amino acid residues are in black. “Microsomal” represents themicrosome-type desaturases, “Chloroplast” represents the chloroplast-typedesaturases.
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fig2: Comparison of the three conserved histidine-rich motifs of proteinsfrom cyanobacteria, eukaryotic algae, and higher plants, including Δ12 fattyacid desaturase, Δ15 fatty acid desaturase, β-carotene ketolase, β-carotenehydroxylase, hydrocarbon oxygenase, Δ12 fatty acid epoxygenase, Δ12 fatty acidacetylenase, Δ12 fatty acid conjugase, and Δ12 fatty acid hydroxylase. Theconserved amino acid residues are in black. “Microsomal” represents themicrosome-type desaturases, “Chloroplast” represents the chloroplast-typedesaturases.

Mentions: There were three typical histidine-rich motifs existed inall the proteins similar to proven cyanobacterial fatty acid desaturases (Table 3). Moreover, there were different conserved residues in the same histidine-boxes of different kinds of proteins, suggesting that these proteinsmight have acquired different functions from a common ancestor during theevolution. According to the different conserved residues of threehistidine-motifs and phylogenetic profile, 16 β-carotene ketolases, 36 β-carotenehydroxylases, and 8 hydrocarbon oxygenases (MocD, a rhizopine oxygenase for the conversion of 3-O-MSI to SI)) were identified from the 37 cyanobacterial genomes(Figures 2, 4, and 5).


Comparative analysis of fatty acid desaturases in cyanobacterial genomes.

Chi X, Yang Q, Zhao F, Qin S, Yang Y, Shen J, Lin H - Comp. Funct. Genomics (2008)

Comparison of the three conserved histidine-rich motifs of proteinsfrom cyanobacteria, eukaryotic algae, and higher plants, including Δ12 fattyacid desaturase, Δ15 fatty acid desaturase, β-carotene ketolase, β-carotenehydroxylase, hydrocarbon oxygenase, Δ12 fatty acid epoxygenase, Δ12 fatty acidacetylenase, Δ12 fatty acid conjugase, and Δ12 fatty acid hydroxylase. Theconserved amino acid residues are in black. “Microsomal” represents themicrosome-type desaturases, “Chloroplast” represents the chloroplast-typedesaturases.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2593844&req=5

fig2: Comparison of the three conserved histidine-rich motifs of proteinsfrom cyanobacteria, eukaryotic algae, and higher plants, including Δ12 fattyacid desaturase, Δ15 fatty acid desaturase, β-carotene ketolase, β-carotenehydroxylase, hydrocarbon oxygenase, Δ12 fatty acid epoxygenase, Δ12 fatty acidacetylenase, Δ12 fatty acid conjugase, and Δ12 fatty acid hydroxylase. Theconserved amino acid residues are in black. “Microsomal” represents themicrosome-type desaturases, “Chloroplast” represents the chloroplast-typedesaturases.
Mentions: There were three typical histidine-rich motifs existed inall the proteins similar to proven cyanobacterial fatty acid desaturases (Table 3). Moreover, there were different conserved residues in the same histidine-boxes of different kinds of proteins, suggesting that these proteinsmight have acquired different functions from a common ancestor during theevolution. According to the different conserved residues of threehistidine-motifs and phylogenetic profile, 16 β-carotene ketolases, 36 β-carotenehydroxylases, and 8 hydrocarbon oxygenases (MocD, a rhizopine oxygenase for the conversion of 3-O-MSI to SI)) were identified from the 37 cyanobacterial genomes(Figures 2, 4, and 5).

Bottom Line: The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont.Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats.Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

ABSTRACT
Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N(2)-fixing cyanobacteria usually possess more types of fatty acid desaturases than that of unicellular species. The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont. Strain Gloeobacter violaceus PCC 7421 was isolated from calcareous rock and lacks thylakoid membranes. The types and amounts of desaturases of this strain are distinct to those of other cyanobacteria, reflecting the earliest divergence of it from the cyanobacterial line. Three thermophilic unicellular strains, Thermosynechococcus elongatus BP-1 and two Synechococcus Yellowstone species, lack highly unsaturated fatty acids in lipids and contain only one Delta9 desaturase in contrast with mesophilic strains, which is probably due to their thermic habitats. Thus, the amounts and types of fatty acid desaturases are various among different cyanobacterial species, which may result from the adaption to environments in evolution.

No MeSH data available.


Related in: MedlinePlus