Limits...
Rapid and reliable diagnosis of murine myeloid leukemia (ML) by FISH of peripheral blood smear using probe of PU. 1, a candidate ML tumor suppressor.

Kanda R, Tsuji S, Ohmachi Y, Ishida Y, Ban N, Shimada Y - Mol Cytogenet (2008)

Bottom Line: This disease has been cytogenetically characterized by a partial deletion of chromosome 2 with G-banding.There was a good correlation between the one-PU.1 frequency in spleen metaphase cells and that in spleen interphase cells (r = 0.96) and between one-PU.1 frequency in spleen interphase cells and that in blood cells (r = 0.83).The FISH method was capable of detecting aberration of copy number of the PU.1 gene on murine chromosome 2, and using a peripheral blood smear is more practical and less invasive than conventional pathological diagnosis or the cytogenetic examination of spleen cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Japan. kanda_r_@nirs.go.jp.

ABSTRACT

Background: Murine myeloid leukemia (ML) provides a good animal model to study the mechanisms of radiation-induced leukemia in humans. This disease has been cytogenetically characterized by a partial deletion of chromosome 2 with G-banding. For the rapid diagnosis of ML, this study reports a FISH method using spleen cells and peripheral blood smears from ML mice exposed to gamma rays and neutrons with PU.1, a candidate ML tumor suppressor, as a probe.

Results: Among mice that were tentatively diagnosed with ML by clinical findings and blood smear examination, 85% carried spleen cells showing the loss of PU.1 although the frequency of these abnormal cells varied among individuals. Mice with very low frequencies of cells showing the loss of one copy of PU.1 (one-PU.1 frequency) were later diagnosed pathologically not with ML but with blastic or eosinophilic leukemia. Some neutron-irradiated mice had cells showing translocated PU.1, although no pathological features differentiated these ML mice from ML mice expressing the simple loss of PU.1.The one-PU.1 frequency can be detected from spleen metaphase cells, spleen interphase cells, and blood smears. There was a good correlation between the one-PU.1 frequency in spleen metaphase cells and that in spleen interphase cells (r = 0.96) and between one-PU.1 frequency in spleen interphase cells and that in blood cells (r = 0.83).

Conclusion: The FISH method was capable of detecting aberration of copy number of the PU.1 gene on murine chromosome 2, and using a peripheral blood smear is more practical and less invasive than conventional pathological diagnosis or the cytogenetic examination of spleen cells.

No MeSH data available.


Related in: MedlinePlus

Comparison of the frequency of cells with one signal of PU.1 and 2 signals of the centromere of chromosome 2 in radiation-induce myeloid leukemia mice. Correlation between the frequency of cells showing a loss of one copy of PU.1 in spleen metaphase cells and that in spleen interphase cells (a) and between the frequencies in spleen interphase cells and that in blood cells (b). The some points were superimposed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2572613&req=5

Figure 2: Comparison of the frequency of cells with one signal of PU.1 and 2 signals of the centromere of chromosome 2 in radiation-induce myeloid leukemia mice. Correlation between the frequency of cells showing a loss of one copy of PU.1 in spleen metaphase cells and that in spleen interphase cells (a) and between the frequencies in spleen interphase cells and that in blood cells (b). The some points were superimposed.

Mentions: Next, metaphase and interphase analyses were compared quantitatively. In this experiment, mice were diagnosed with ML not only by clinical findings and blood smear examination but also by flow cytometry and pathological investigations to clarify the relation of frequency of G1R2 cells and clinical diagnosis. The percentages of G1R2 cells varied from 0 to 100% (Fig. 2), but cells with 2 red and 0 green signals, i.e. with the loss of both PU.1 signals, were not observed. There was a good correlation between the percentage of cells with one PU.1 signal in the metaphase and interphase specimens (r = 0.96), which suggested the high reliability of the results obtained by interphase FISH.


Rapid and reliable diagnosis of murine myeloid leukemia (ML) by FISH of peripheral blood smear using probe of PU. 1, a candidate ML tumor suppressor.

Kanda R, Tsuji S, Ohmachi Y, Ishida Y, Ban N, Shimada Y - Mol Cytogenet (2008)

Comparison of the frequency of cells with one signal of PU.1 and 2 signals of the centromere of chromosome 2 in radiation-induce myeloid leukemia mice. Correlation between the frequency of cells showing a loss of one copy of PU.1 in spleen metaphase cells and that in spleen interphase cells (a) and between the frequencies in spleen interphase cells and that in blood cells (b). The some points were superimposed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2572613&req=5

Figure 2: Comparison of the frequency of cells with one signal of PU.1 and 2 signals of the centromere of chromosome 2 in radiation-induce myeloid leukemia mice. Correlation between the frequency of cells showing a loss of one copy of PU.1 in spleen metaphase cells and that in spleen interphase cells (a) and between the frequencies in spleen interphase cells and that in blood cells (b). The some points were superimposed.
Mentions: Next, metaphase and interphase analyses were compared quantitatively. In this experiment, mice were diagnosed with ML not only by clinical findings and blood smear examination but also by flow cytometry and pathological investigations to clarify the relation of frequency of G1R2 cells and clinical diagnosis. The percentages of G1R2 cells varied from 0 to 100% (Fig. 2), but cells with 2 red and 0 green signals, i.e. with the loss of both PU.1 signals, were not observed. There was a good correlation between the percentage of cells with one PU.1 signal in the metaphase and interphase specimens (r = 0.96), which suggested the high reliability of the results obtained by interphase FISH.

Bottom Line: This disease has been cytogenetically characterized by a partial deletion of chromosome 2 with G-banding.There was a good correlation between the one-PU.1 frequency in spleen metaphase cells and that in spleen interphase cells (r = 0.96) and between one-PU.1 frequency in spleen interphase cells and that in blood cells (r = 0.83).The FISH method was capable of detecting aberration of copy number of the PU.1 gene on murine chromosome 2, and using a peripheral blood smear is more practical and less invasive than conventional pathological diagnosis or the cytogenetic examination of spleen cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Japan. kanda_r_@nirs.go.jp.

ABSTRACT

Background: Murine myeloid leukemia (ML) provides a good animal model to study the mechanisms of radiation-induced leukemia in humans. This disease has been cytogenetically characterized by a partial deletion of chromosome 2 with G-banding. For the rapid diagnosis of ML, this study reports a FISH method using spleen cells and peripheral blood smears from ML mice exposed to gamma rays and neutrons with PU.1, a candidate ML tumor suppressor, as a probe.

Results: Among mice that were tentatively diagnosed with ML by clinical findings and blood smear examination, 85% carried spleen cells showing the loss of PU.1 although the frequency of these abnormal cells varied among individuals. Mice with very low frequencies of cells showing the loss of one copy of PU.1 (one-PU.1 frequency) were later diagnosed pathologically not with ML but with blastic or eosinophilic leukemia. Some neutron-irradiated mice had cells showing translocated PU.1, although no pathological features differentiated these ML mice from ML mice expressing the simple loss of PU.1.The one-PU.1 frequency can be detected from spleen metaphase cells, spleen interphase cells, and blood smears. There was a good correlation between the one-PU.1 frequency in spleen metaphase cells and that in spleen interphase cells (r = 0.96) and between one-PU.1 frequency in spleen interphase cells and that in blood cells (r = 0.83).

Conclusion: The FISH method was capable of detecting aberration of copy number of the PU.1 gene on murine chromosome 2, and using a peripheral blood smear is more practical and less invasive than conventional pathological diagnosis or the cytogenetic examination of spleen cells.

No MeSH data available.


Related in: MedlinePlus