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Fast quantitative determination of microbial rhamnolipids from cultivation broths by ATR-FTIR Spectroscopy.

Leitermann F, Syldatk C, Hausmann R - J Biol Eng (2008)

Bottom Line: Even better accuracies between 0.28 g/l - 0.59 g/l were found for independent test samples of an arbitrarily selected cultivation.ATR-FTIR was found to be suitable for the rapid analysis of rhamnolipids in a biotechnological process with good reproducibility in sample determination and sufficient accuracy.An improvement in accuracy through continuous expansion and validation of the reference spectra set seems very likely.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research University Karlsruhe, Institute of Engineering in Life Sciences, Section of Technical Biology, Engler-Bunte-Ring 1, 76131 Karlsruhe, Germany. Rudolf.Hausmann@tebi.uni-karlsruhe.de.

ABSTRACT

Background: Vibrational spectroscopic techniques are becoming increasingly important and popular because they have the potential to provide rapid and convenient solutions to routine analytical problems. Using these techniques, a variety of substances can be characterized, identified and also quantified rapidly.

Results: The rapid ATR-FTIR (Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy) in time technique has been applied, which is suitable to quantify the concentrations of microbial rhamnolipids in a typical cultivation process. While the usually applied HPLC analysis requires an extensive and time consuming multi step extraction protocol for sample preparation, the ATR-FTIR-method allows the quantification of the rhamnolipids within 20 minutes. Accuracies between 0.5 g/l - 2.1 g/l for the different analytes were determined by cross validation of the calibration set. Even better accuracies between 0.28 g/l - 0.59 g/l were found for independent test samples of an arbitrarily selected cultivation.

Conclusion: ATR-FTIR was found to be suitable for the rapid analysis of rhamnolipids in a biotechnological process with good reproducibility in sample determination and sufficient accuracy. An improvement in accuracy through continuous expansion and validation of the reference spectra set seems very likely.

No MeSH data available.


Reproducibility of spectra measurements. This figure displays four spectra of two fermentation derived samples after double application. A comparison of the corresponding two spectra of each sample showed correlations of above 99.9%.
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Figure 2: Reproducibility of spectra measurements. This figure displays four spectra of two fermentation derived samples after double application. A comparison of the corresponding two spectra of each sample showed correlations of above 99.9%.

Mentions: For verification of the reproducibility of the application method, two samples of the cultivation broth were prepared and measured twice. The corresponding spectra are shown in Figure 2. The comparison of the corresponding spectra only showed minimal diversities, relating to the spectra intensities., A spectral match was found to be above 99.99%, for the first sample and 99.96%, for the second sample.


Fast quantitative determination of microbial rhamnolipids from cultivation broths by ATR-FTIR Spectroscopy.

Leitermann F, Syldatk C, Hausmann R - J Biol Eng (2008)

Reproducibility of spectra measurements. This figure displays four spectra of two fermentation derived samples after double application. A comparison of the corresponding two spectra of each sample showed correlations of above 99.9%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2572035&req=5

Figure 2: Reproducibility of spectra measurements. This figure displays four spectra of two fermentation derived samples after double application. A comparison of the corresponding two spectra of each sample showed correlations of above 99.9%.
Mentions: For verification of the reproducibility of the application method, two samples of the cultivation broth were prepared and measured twice. The corresponding spectra are shown in Figure 2. The comparison of the corresponding spectra only showed minimal diversities, relating to the spectra intensities., A spectral match was found to be above 99.99%, for the first sample and 99.96%, for the second sample.

Bottom Line: Even better accuracies between 0.28 g/l - 0.59 g/l were found for independent test samples of an arbitrarily selected cultivation.ATR-FTIR was found to be suitable for the rapid analysis of rhamnolipids in a biotechnological process with good reproducibility in sample determination and sufficient accuracy.An improvement in accuracy through continuous expansion and validation of the reference spectra set seems very likely.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research University Karlsruhe, Institute of Engineering in Life Sciences, Section of Technical Biology, Engler-Bunte-Ring 1, 76131 Karlsruhe, Germany. Rudolf.Hausmann@tebi.uni-karlsruhe.de.

ABSTRACT

Background: Vibrational spectroscopic techniques are becoming increasingly important and popular because they have the potential to provide rapid and convenient solutions to routine analytical problems. Using these techniques, a variety of substances can be characterized, identified and also quantified rapidly.

Results: The rapid ATR-FTIR (Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy) in time technique has been applied, which is suitable to quantify the concentrations of microbial rhamnolipids in a typical cultivation process. While the usually applied HPLC analysis requires an extensive and time consuming multi step extraction protocol for sample preparation, the ATR-FTIR-method allows the quantification of the rhamnolipids within 20 minutes. Accuracies between 0.5 g/l - 2.1 g/l for the different analytes were determined by cross validation of the calibration set. Even better accuracies between 0.28 g/l - 0.59 g/l were found for independent test samples of an arbitrarily selected cultivation.

Conclusion: ATR-FTIR was found to be suitable for the rapid analysis of rhamnolipids in a biotechnological process with good reproducibility in sample determination and sufficient accuracy. An improvement in accuracy through continuous expansion and validation of the reference spectra set seems very likely.

No MeSH data available.