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Molecular epidemiology of HCV monoinfection and HIV/HCV coinfection in injection drug users in Liuzhou, Southern China.

Tan Y, Wei QH, Chen LJ, Chan PC, Lai WS, He ML, Kung HF, Lee SS - PLoS ONE (2008)

Bottom Line: Phylogenetic analysis revealed that genotype 6a was predominant in the study population.There were shorter genetic distances among the 6a sequences compared to the other HCV subtypes-1a, 3a, and 3b.This pattern is different from that in other South East Asian countries where HCV infections have probably predated HIV.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.

ABSTRACT

Background: Hepatitis C virus (HCV) mono-infection and HCV/HIV (human immunodeficiency virus) co-infection are growing problems in injection drug users (IDU). Their prevalence and genotypic patterns vary with geographic locations. Access to harm reduction measures is opening up opportunities for improving the HIV/HCV profiling of IDU in China, where IDUs account for a significant proportion of the two infections especially in the southern part of the country.

Methodology/principal findings: A cross sectional study was conducted. Through the Liuzhou Methadone Clinic, a total of 117 injection drug users (IDUs) were recruited from Guangxi, Southern China. A majority of the IDUs (96%) were HCV antibody positive, of which 21% were HIV infected. Unlike HCV monoinfection, there was spatial heterogeneity in the distribution of HIV/HCV coinfection, the latter also characterized by a higher prevalence of needle-sharing. Phylogenetic analysis revealed that genotype 6a was predominant in the study population. There were shorter genetic distances among the 6a sequences compared to the other HCV subtypes-1a, 3a, and 3b.

Conclusion/significance: The results suggested that HIV and HCV were introduced at around the same time to the IDU populations in Southern China, followed by their differential spread as determined by the biologic characteristics of the virus and the intensity of behavioural risk. This pattern is different from that in other South East Asian countries where HCV infections have probably predated HIV.

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Related in: MedlinePlus

HCV genotype 6a in the study population (n = 34) and other countries and regions.Codes for sources of samples: CN = China; TW = Taiwan; HK = Hong Kong VN = Vietnam.
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pone-0003608-g003: HCV genotype 6a in the study population (n = 34) and other countries and regions.Codes for sources of samples: CN = China; TW = Taiwan; HK = Hong Kong VN = Vietnam.

Mentions: Figure 2 shows the phylogenetic tree of 96 sequences and reference sequences. The sequences of coinfection IDUs were evenly distributed in the whole tree. There is no evidence of clustering in HIV coinfected patients. Comparing between different genotypes, the genetic distances obtained from MEGA 3.0 within genotype 6 (0.125±0.012) were shorter than those within genotype 1 (0.237±0.021) and genotype 3 (0.168±0.017). The 6a sequences from Liuhzou were aligned with other 6a sequences randomly selected from the Genebank. Overall, there's a high degree of sequence identity among the 6a samples in Liuzhou, than between sequences in this study and those in neighbouring cities/countries in Hong Kong, Tauiwan, Vietnam and China (Figure 3).


Molecular epidemiology of HCV monoinfection and HIV/HCV coinfection in injection drug users in Liuzhou, Southern China.

Tan Y, Wei QH, Chen LJ, Chan PC, Lai WS, He ML, Kung HF, Lee SS - PLoS ONE (2008)

HCV genotype 6a in the study population (n = 34) and other countries and regions.Codes for sources of samples: CN = China; TW = Taiwan; HK = Hong Kong VN = Vietnam.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2571986&req=5

pone-0003608-g003: HCV genotype 6a in the study population (n = 34) and other countries and regions.Codes for sources of samples: CN = China; TW = Taiwan; HK = Hong Kong VN = Vietnam.
Mentions: Figure 2 shows the phylogenetic tree of 96 sequences and reference sequences. The sequences of coinfection IDUs were evenly distributed in the whole tree. There is no evidence of clustering in HIV coinfected patients. Comparing between different genotypes, the genetic distances obtained from MEGA 3.0 within genotype 6 (0.125±0.012) were shorter than those within genotype 1 (0.237±0.021) and genotype 3 (0.168±0.017). The 6a sequences from Liuhzou were aligned with other 6a sequences randomly selected from the Genebank. Overall, there's a high degree of sequence identity among the 6a samples in Liuzhou, than between sequences in this study and those in neighbouring cities/countries in Hong Kong, Tauiwan, Vietnam and China (Figure 3).

Bottom Line: Phylogenetic analysis revealed that genotype 6a was predominant in the study population.There were shorter genetic distances among the 6a sequences compared to the other HCV subtypes-1a, 3a, and 3b.This pattern is different from that in other South East Asian countries where HCV infections have probably predated HIV.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.

ABSTRACT

Background: Hepatitis C virus (HCV) mono-infection and HCV/HIV (human immunodeficiency virus) co-infection are growing problems in injection drug users (IDU). Their prevalence and genotypic patterns vary with geographic locations. Access to harm reduction measures is opening up opportunities for improving the HIV/HCV profiling of IDU in China, where IDUs account for a significant proportion of the two infections especially in the southern part of the country.

Methodology/principal findings: A cross sectional study was conducted. Through the Liuzhou Methadone Clinic, a total of 117 injection drug users (IDUs) were recruited from Guangxi, Southern China. A majority of the IDUs (96%) were HCV antibody positive, of which 21% were HIV infected. Unlike HCV monoinfection, there was spatial heterogeneity in the distribution of HIV/HCV coinfection, the latter also characterized by a higher prevalence of needle-sharing. Phylogenetic analysis revealed that genotype 6a was predominant in the study population. There were shorter genetic distances among the 6a sequences compared to the other HCV subtypes-1a, 3a, and 3b.

Conclusion/significance: The results suggested that HIV and HCV were introduced at around the same time to the IDU populations in Southern China, followed by their differential spread as determined by the biologic characteristics of the virus and the intensity of behavioural risk. This pattern is different from that in other South East Asian countries where HCV infections have probably predated HIV.

Show MeSH
Related in: MedlinePlus