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Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

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Related in: MedlinePlus

Notations for representing the CID fragmentation sites along the backbone of PAMAM dendrimers (only half of the molecule is shown).
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grs3: Notations for representing the CID fragmentation sites along the backbone of PAMAM dendrimers (only half of the molecule is shown).

Mentions: CID fragments were assigned according to the nomenclature shown in Scheme 3. Assignments are given in form AGn, BGn, and CGn, where subscript Gn refers to the generation in which cleavage takes place. Note that BGn is equivalent to ECD assignment Gn(Kout), but the multiplicity of the CID fragmentation (see following text) demands simpler nomenclature.


Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Notations for representing the CID fragmentation sites along the backbone of PAMAM dendrimers (only half of the molecule is shown).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571976&req=5

grs3: Notations for representing the CID fragmentation sites along the backbone of PAMAM dendrimers (only half of the molecule is shown).
Mentions: CID fragments were assigned according to the nomenclature shown in Scheme 3. Assignments are given in form AGn, BGn, and CGn, where subscript Gn refers to the generation in which cleavage takes place. Note that BGn is equivalent to ECD assignment Gn(Kout), but the multiplicity of the CID fragmentation (see following text) demands simpler nomenclature.

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

Show MeSH
Related in: MedlinePlus