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Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

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Notations for representing ECD cleavage sites along the backbone of PAMAM dendrimers.
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grs2: Notations for representing ECD cleavage sites along the backbone of PAMAM dendrimers.

Mentions: ECD fragments were assigned based on the nomenclature devised by Oh and colleagues [17] (see Scheme 2). Assignments are given in the form Gn(m), where subscript n refers to the generation in which fragmentation takes place and m describes the type of fragmentation; a•/x, b/y, c/z•. For example, fragment G1(y) was derived from cleavage of the amide bond in generation 1. Gn(in) and Gn(out) notations concern fragmentation that takes place core-side of the tertiary amines. K refers to cleavages surface-side of the tertiary amines.


Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Notations for representing ECD cleavage sites along the backbone of PAMAM dendrimers.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571976&req=5

grs2: Notations for representing ECD cleavage sites along the backbone of PAMAM dendrimers.
Mentions: ECD fragments were assigned based on the nomenclature devised by Oh and colleagues [17] (see Scheme 2). Assignments are given in the form Gn(m), where subscript n refers to the generation in which fragmentation takes place and m describes the type of fragmentation; a•/x, b/y, c/z•. For example, fragment G1(y) was derived from cleavage of the amide bond in generation 1. Gn(in) and Gn(out) notations concern fragmentation that takes place core-side of the tertiary amines. K refers to cleavages surface-side of the tertiary amines.

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

Show MeSH
Related in: MedlinePlus