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Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

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Structure of a PAMAM dendrimer.
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grs1: Structure of a PAMAM dendrimer.

Mentions: Polyamidoamine (PAMAM) dendrimers, introduced by Tomalia in 1994 [1], are a class of polymers with a high degree of molecular uniformity and a highly functionalized terminal surface. They are characterized by three components: the central core, branches, and surface groups (Scheme 1). Each subsequent growth step represents a new generation of polymer with increasing molecular size, mass, and number of surface groups. Dendrimers allow precise control of size, shape, and placement of functional groups [2, 3], PAMAM dendrimers are often referred to as “artificial proteins” [4] because they closely match the sizes and contours of many proteins. Because of the high level of control over the architecture of dendrimers, these species play important roles as carriers in many biomedical applications including drug delivery, gene transfection, and imaging [4–7].


Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.

Kaczorowska MA, Cooper HJ - J. Am. Soc. Mass Spectrom. (2008)

Structure of a PAMAM dendrimer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571976&req=5

grs1: Structure of a PAMAM dendrimer.
Mentions: Polyamidoamine (PAMAM) dendrimers, introduced by Tomalia in 1994 [1], are a class of polymers with a high degree of molecular uniformity and a highly functionalized terminal surface. They are characterized by three components: the central core, branches, and surface groups (Scheme 1). Each subsequent growth step represents a new generation of polymer with increasing molecular size, mass, and number of surface groups. Dendrimers allow precise control of size, shape, and placement of functional groups [2, 3], PAMAM dendrimers are often referred to as “artificial proteins” [4] because they closely match the sizes and contours of many proteins. Because of the high level of control over the architecture of dendrimers, these species play important roles as carriers in many biomedical applications including drug delivery, gene transfection, and imaging [4–7].

Bottom Line: ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra.CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation.The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT
Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.

Show MeSH
Related in: MedlinePlus