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The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.

Pickering C, Hägglund M, Szmydynger-Chodobska J, Marques F, Palha JA, Waller L, Chodobski A, Fredriksson R, Lagerström MC, Schiöth HB - BMC Neurosci (2008)

Bottom Line: A single copy of GPR125 was found in many vertebrate genomes.Induction of inflammation by LPS did not change GPR125 expression.However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

View Article: PubMed Central - HTML - PubMed

Affiliation: Uppsala University, Department of Neuroscience, Functional Pharmacology, BMC, Box 593, SE-75124, Uppsala, Sweden. chris.pickering@neuro.uu.se

ABSTRACT

Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.

Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.

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Immunohistochemistry using an antibody directed against the GPR125 protein (green). GPR125 was again localized to the choroid plexus cells using the epithelial cell-specific marker cytokeratin (red). This specific staining was observed in sections from the brain of a 3 week old rat, thus confirming expression of GPR125 in pre-adolescence.
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Figure 5: Immunohistochemistry using an antibody directed against the GPR125 protein (green). GPR125 was again localized to the choroid plexus cells using the epithelial cell-specific marker cytokeratin (red). This specific staining was observed in sections from the brain of a 3 week old rat, thus confirming expression of GPR125 in pre-adolescence.

Mentions: To demonstrate localization of the GPR125 protein in the choroid plexus, sections were labeled with the GPR125 antibody (Figure 5). Using the epithelial cell-specific cytokeratin antibody and the z-stack function of the confocal microscope, GPR125 staining (green) was observed in the central portion of the choroid plexus with some overlap with the cytokeratin staining (red) which marks the barrier region of the choroid plexus (Figure 5, merged). Localization of this in the choroid plexus from a 3-week-old animal also indicates central nervous system expression in pre-adolescence. GPR125 did not, however, co-localize with the cerebral vasculature (Additional file 4) as illustrated by double staining with the monocarboxylate transporter MCT-2 which labels the cerebral vasculature and the walls of the ventricle [24].


The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.

Pickering C, Hägglund M, Szmydynger-Chodobska J, Marques F, Palha JA, Waller L, Chodobski A, Fredriksson R, Lagerström MC, Schiöth HB - BMC Neurosci (2008)

Immunohistochemistry using an antibody directed against the GPR125 protein (green). GPR125 was again localized to the choroid plexus cells using the epithelial cell-specific marker cytokeratin (red). This specific staining was observed in sections from the brain of a 3 week old rat, thus confirming expression of GPR125 in pre-adolescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571103&req=5

Figure 5: Immunohistochemistry using an antibody directed against the GPR125 protein (green). GPR125 was again localized to the choroid plexus cells using the epithelial cell-specific marker cytokeratin (red). This specific staining was observed in sections from the brain of a 3 week old rat, thus confirming expression of GPR125 in pre-adolescence.
Mentions: To demonstrate localization of the GPR125 protein in the choroid plexus, sections were labeled with the GPR125 antibody (Figure 5). Using the epithelial cell-specific cytokeratin antibody and the z-stack function of the confocal microscope, GPR125 staining (green) was observed in the central portion of the choroid plexus with some overlap with the cytokeratin staining (red) which marks the barrier region of the choroid plexus (Figure 5, merged). Localization of this in the choroid plexus from a 3-week-old animal also indicates central nervous system expression in pre-adolescence. GPR125 did not, however, co-localize with the cerebral vasculature (Additional file 4) as illustrated by double staining with the monocarboxylate transporter MCT-2 which labels the cerebral vasculature and the walls of the ventricle [24].

Bottom Line: A single copy of GPR125 was found in many vertebrate genomes.Induction of inflammation by LPS did not change GPR125 expression.However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

View Article: PubMed Central - HTML - PubMed

Affiliation: Uppsala University, Department of Neuroscience, Functional Pharmacology, BMC, Box 593, SE-75124, Uppsala, Sweden. chris.pickering@neuro.uu.se

ABSTRACT

Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.

Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.

Show MeSH
Related in: MedlinePlus