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The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.

Pickering C, Hägglund M, Szmydynger-Chodobska J, Marques F, Palha JA, Waller L, Chodobski A, Fredriksson R, Lagerström MC, Schiöth HB - BMC Neurosci (2008)

Bottom Line: A single copy of GPR125 was found in many vertebrate genomes.Induction of inflammation by LPS did not change GPR125 expression.However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

View Article: PubMed Central - HTML - PubMed

Affiliation: Uppsala University, Department of Neuroscience, Functional Pharmacology, BMC, Box 593, SE-75124, Uppsala, Sweden. chris.pickering@neuro.uu.se

ABSTRACT

Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.

Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.

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Related in: MedlinePlus

A) Localization of GPR125 using qPCR in mouse brain and peripheral tissues. Values are expressed relative to the minimum expression value for this analysis (colliculus). B) GPR125 expression was not changed following induction of an inflammatory response via LPS injection. C) GPR125 expression was affected by TBI. GPR125 in the choroid plexus was upregulated at 4 h after injury while the upregulation in the hippocampus did not occur until 1 day post-TBI, suggesting a role of the CSF pathways in post-traumatic regulation of GPR125 expression in the choroid plexus and hippocampus.
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Figure 2: A) Localization of GPR125 using qPCR in mouse brain and peripheral tissues. Values are expressed relative to the minimum expression value for this analysis (colliculus). B) GPR125 expression was not changed following induction of an inflammatory response via LPS injection. C) GPR125 expression was affected by TBI. GPR125 in the choroid plexus was upregulated at 4 h after injury while the upregulation in the hippocampus did not occur until 1 day post-TBI, suggesting a role of the CSF pathways in post-traumatic regulation of GPR125 expression in the choroid plexus and hippocampus.

Mentions: To obtain an overview of the expression of mRNA for GPR125, qPCR was performed on a panel of mouse brain regions and peripheral organs (Figure 2A). To simplify presentation, values were normalized to the tissue with minimum expression of GPR125 (colliculus = 1). Expression in most brain tissues was approximately 2-fold that of the colliculus such that no differential pattern of expression was observed between striatum, hippocampus or thalamus. In particular, cortical expression of GPR125 was nearly 8-fold higher than that of the colliculus while hypothalamic expression was roughly 3.5-fold higher. Expression in the periphery was higher compared to the brain (numbers in parentheses denote fold-values with respect to colliculus); heart (2.5), liver (3.6), kidney (6.8) and pancreas (7). Highest overall expression for GPR125 was observed in the lungs (18-fold that of colliculus).


The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.

Pickering C, Hägglund M, Szmydynger-Chodobska J, Marques F, Palha JA, Waller L, Chodobski A, Fredriksson R, Lagerström MC, Schiöth HB - BMC Neurosci (2008)

A) Localization of GPR125 using qPCR in mouse brain and peripheral tissues. Values are expressed relative to the minimum expression value for this analysis (colliculus). B) GPR125 expression was not changed following induction of an inflammatory response via LPS injection. C) GPR125 expression was affected by TBI. GPR125 in the choroid plexus was upregulated at 4 h after injury while the upregulation in the hippocampus did not occur until 1 day post-TBI, suggesting a role of the CSF pathways in post-traumatic regulation of GPR125 expression in the choroid plexus and hippocampus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571103&req=5

Figure 2: A) Localization of GPR125 using qPCR in mouse brain and peripheral tissues. Values are expressed relative to the minimum expression value for this analysis (colliculus). B) GPR125 expression was not changed following induction of an inflammatory response via LPS injection. C) GPR125 expression was affected by TBI. GPR125 in the choroid plexus was upregulated at 4 h after injury while the upregulation in the hippocampus did not occur until 1 day post-TBI, suggesting a role of the CSF pathways in post-traumatic regulation of GPR125 expression in the choroid plexus and hippocampus.
Mentions: To obtain an overview of the expression of mRNA for GPR125, qPCR was performed on a panel of mouse brain regions and peripheral organs (Figure 2A). To simplify presentation, values were normalized to the tissue with minimum expression of GPR125 (colliculus = 1). Expression in most brain tissues was approximately 2-fold that of the colliculus such that no differential pattern of expression was observed between striatum, hippocampus or thalamus. In particular, cortical expression of GPR125 was nearly 8-fold higher than that of the colliculus while hypothalamic expression was roughly 3.5-fold higher. Expression in the periphery was higher compared to the brain (numbers in parentheses denote fold-values with respect to colliculus); heart (2.5), liver (3.6), kidney (6.8) and pancreas (7). Highest overall expression for GPR125 was observed in the lungs (18-fold that of colliculus).

Bottom Line: A single copy of GPR125 was found in many vertebrate genomes.Induction of inflammation by LPS did not change GPR125 expression.However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

View Article: PubMed Central - HTML - PubMed

Affiliation: Uppsala University, Department of Neuroscience, Functional Pharmacology, BMC, Box 593, SE-75124, Uppsala, Sweden. chris.pickering@neuro.uu.se

ABSTRACT

Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.

Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.

Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.

Show MeSH
Related in: MedlinePlus