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Syzygium jambolanum treatment improves survival in lethal sepsis induced in mice.

Maciel MC, Farias JC, Maluf MJ, Gomes EA, Pereira PV, Aragão-Filho WC, Frazão JB, Costa GC, Sousa SM, Silva LA, Amaral FM, Russo M, Guerra RN, Nascimento FR - BMC Complement Altern Med (2008)

Bottom Line: Despite the increased migration and activation of peritoneal cells the HCE treatment did not decrease the number of CFU.The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.We conclude that the treatment with S. jambolanum has a potent prophylactic anti-septic effect that is not associated to a direct microbicidal effect but it is associated to a recruitment of activated neutrophils to the infectious site and to a diminished systemic inflammatory response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Immunophysiology, Federal University of Maranhão, São Luís, MA, Brazil. macielmcg@yahoo.com.

ABSTRACT

Background: The leaves and the fruits from Syzygium jambolanum DC.(Myrtaceae), a plant known in Brazil as sweet olive or 'jambolão', have been used by native people to treat infectious diseases, diabetes, and stomachache. Since the bactericidal activity of S. jambolanum has been confirmed in vitro, the aim of this work was to evaluate the effect of the prophylactic treatment with S. jambolanum on the in vivo polymicrobial infection induced by cecal ligation and puncture (CLP) in mice.

Methods: C57Bl/6 mice were treated by the subcutaneous route with a hydroalcoholic extract from fresh leaves of S. jambolanum (HCE). After 6 h, a bacterial infection was induced in the peritoneum using the lethal CLP model. The mice were killed 12 h after the CLP induction to evaluate the cellular influx and local and systemic inflammatory mediators' production. Some animals were maintained alive to evaluate the survival rate.

Results: The prophylactic HCE treatment increased the mice survival, the neutrophil migration to infectious site, the spreading ability and the hydrogen peroxide release, but decreased the serum TNF and nitrite. Despite the increased migration and activation of peritoneal cells the HCE treatment did not decrease the number of CFU. The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.

Conclusion: We conclude that the treatment with S. jambolanum has a potent prophylactic anti-septic effect that is not associated to a direct microbicidal effect but it is associated to a recruitment of activated neutrophils to the infectious site and to a diminished systemic inflammatory response.

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Total and differential cell counting in the peritoneal cavity and inflammatory infiltration in the cecum wall. The total and differential counting of peritoneal cells were performed 12 h after the CLP (A). The results were expressed as mean ± S.E.M (10 animals/group). The cecum of the control group (B) or of the HCE 5 group (C) was obtained to evaluate the histopathology at a 400× magnification. *p < 0.05 when compared to sham and # when compared to the control group.
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Figure 2: Total and differential cell counting in the peritoneal cavity and inflammatory infiltration in the cecum wall. The total and differential counting of peritoneal cells were performed 12 h after the CLP (A). The results were expressed as mean ± S.E.M (10 animals/group). The cecum of the control group (B) or of the HCE 5 group (C) was obtained to evaluate the histopathology at a 400× magnification. *p < 0.05 when compared to sham and # when compared to the control group.

Mentions: The cell recruitment to the peritoneal cavity, constituted mainly by neutrophils, was enhanced in the HCE treated mice when compared to the control group (Figure 2A). The treatment with HCE also induced an intense infiltration of inflammatory cells to the cecum walls which was more evident than that observed in the control group (Figures 2B–C).


Syzygium jambolanum treatment improves survival in lethal sepsis induced in mice.

Maciel MC, Farias JC, Maluf MJ, Gomes EA, Pereira PV, Aragão-Filho WC, Frazão JB, Costa GC, Sousa SM, Silva LA, Amaral FM, Russo M, Guerra RN, Nascimento FR - BMC Complement Altern Med (2008)

Total and differential cell counting in the peritoneal cavity and inflammatory infiltration in the cecum wall. The total and differential counting of peritoneal cells were performed 12 h after the CLP (A). The results were expressed as mean ± S.E.M (10 animals/group). The cecum of the control group (B) or of the HCE 5 group (C) was obtained to evaluate the histopathology at a 400× magnification. *p < 0.05 when compared to sham and # when compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2571085&req=5

Figure 2: Total and differential cell counting in the peritoneal cavity and inflammatory infiltration in the cecum wall. The total and differential counting of peritoneal cells were performed 12 h after the CLP (A). The results were expressed as mean ± S.E.M (10 animals/group). The cecum of the control group (B) or of the HCE 5 group (C) was obtained to evaluate the histopathology at a 400× magnification. *p < 0.05 when compared to sham and # when compared to the control group.
Mentions: The cell recruitment to the peritoneal cavity, constituted mainly by neutrophils, was enhanced in the HCE treated mice when compared to the control group (Figure 2A). The treatment with HCE also induced an intense infiltration of inflammatory cells to the cecum walls which was more evident than that observed in the control group (Figures 2B–C).

Bottom Line: Despite the increased migration and activation of peritoneal cells the HCE treatment did not decrease the number of CFU.The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.We conclude that the treatment with S. jambolanum has a potent prophylactic anti-septic effect that is not associated to a direct microbicidal effect but it is associated to a recruitment of activated neutrophils to the infectious site and to a diminished systemic inflammatory response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Immunophysiology, Federal University of Maranhão, São Luís, MA, Brazil. macielmcg@yahoo.com.

ABSTRACT

Background: The leaves and the fruits from Syzygium jambolanum DC.(Myrtaceae), a plant known in Brazil as sweet olive or 'jambolão', have been used by native people to treat infectious diseases, diabetes, and stomachache. Since the bactericidal activity of S. jambolanum has been confirmed in vitro, the aim of this work was to evaluate the effect of the prophylactic treatment with S. jambolanum on the in vivo polymicrobial infection induced by cecal ligation and puncture (CLP) in mice.

Methods: C57Bl/6 mice were treated by the subcutaneous route with a hydroalcoholic extract from fresh leaves of S. jambolanum (HCE). After 6 h, a bacterial infection was induced in the peritoneum using the lethal CLP model. The mice were killed 12 h after the CLP induction to evaluate the cellular influx and local and systemic inflammatory mediators' production. Some animals were maintained alive to evaluate the survival rate.

Results: The prophylactic HCE treatment increased the mice survival, the neutrophil migration to infectious site, the spreading ability and the hydrogen peroxide release, but decreased the serum TNF and nitrite. Despite the increased migration and activation of peritoneal cells the HCE treatment did not decrease the number of CFU. The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.

Conclusion: We conclude that the treatment with S. jambolanum has a potent prophylactic anti-septic effect that is not associated to a direct microbicidal effect but it is associated to a recruitment of activated neutrophils to the infectious site and to a diminished systemic inflammatory response.

Show MeSH
Related in: MedlinePlus