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Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France.

Desnos-Ollivier M, Bretagne S, Bernède C, Robert V, Raoux D, Chachaty E, Forget E, Lacroix C, Dromer F, Yeasts Gro - Emerging Infect. Dis. (2008)

Bottom Line: Epidemiologic data and gene sequences were analyzed, and molecular typing was performed.Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area.We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Paris, France.

ABSTRACT
Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

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Distribution of 130 Candida tropicalis isolates recovered from blood cultures during the first 4 years of an active surveillance program (YEASTS study) on yeasts fungemia in the Paris area, France (October 2002 through September 2006), according to the MICs of flucytosine determined with the EUCAST microdilution method (4).
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Figure 1: Distribution of 130 Candida tropicalis isolates recovered from blood cultures during the first 4 years of an active surveillance program (YEASTS study) on yeasts fungemia in the Paris area, France (October 2002 through September 2006), according to the MICs of flucytosine determined with the EUCAST microdilution method (4).

Mentions: We analyzed the episodes of fungemia caused by C. tropicalis and recorded during the first 4 years of the YEASTS study; 130 episodes were recorded in 24 of the 27 participating centers. Distribution of flucytosine MICs showed 2 populations, 1 with MICs <2 μg/mL and 1 with MICs >8 μg/mL (Figure). In light of these results, susceptibility to 5FC (S5FC) was defined by an MIC <8 μg/mL and resistance (R5FC) by an MIC >8 μg/mL.


Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France.

Desnos-Ollivier M, Bretagne S, Bernède C, Robert V, Raoux D, Chachaty E, Forget E, Lacroix C, Dromer F, Yeasts Gro - Emerging Infect. Dis. (2008)

Distribution of 130 Candida tropicalis isolates recovered from blood cultures during the first 4 years of an active surveillance program (YEASTS study) on yeasts fungemia in the Paris area, France (October 2002 through September 2006), according to the MICs of flucytosine determined with the EUCAST microdilution method (4).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570934&req=5

Figure 1: Distribution of 130 Candida tropicalis isolates recovered from blood cultures during the first 4 years of an active surveillance program (YEASTS study) on yeasts fungemia in the Paris area, France (October 2002 through September 2006), according to the MICs of flucytosine determined with the EUCAST microdilution method (4).
Mentions: We analyzed the episodes of fungemia caused by C. tropicalis and recorded during the first 4 years of the YEASTS study; 130 episodes were recorded in 24 of the 27 participating centers. Distribution of flucytosine MICs showed 2 populations, 1 with MICs <2 μg/mL and 1 with MICs >8 μg/mL (Figure). In light of these results, susceptibility to 5FC (S5FC) was defined by an MIC <8 μg/mL and resistance (R5FC) by an MIC >8 μg/mL.

Bottom Line: Epidemiologic data and gene sequences were analyzed, and molecular typing was performed.Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area.We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Paris, France.

ABSTRACT
Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

Show MeSH
Related in: MedlinePlus