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Hemorrhagic fever with renal syndrome caused by 2 lineages of Dobrava hantavirus, Russia.

Klempa B, Tkachenko EA, Dzagurova TK, Yunicheva YV, Morozov VG, Okulova NM, Slyusareva GP, Smirnov A, Kruger DH - Emerging Infect. Dis. (2008)

Bottom Line: To determine causative agents, we examined 126 cases of DOBV-associated HFRS in central and southern European Russia.In central Russia (Lipetsk, Voronezh, Orel regions), outbreaks were caused by a DOBV variant (DOBV-Aa) carried by Apodemus agrarius.In southern Russia (Sochi district), where HFRS is endemic, HFRS cases were caused by a new DOBV variant (DOBV-Ap), found in A. ponticus, a novel hantavirus natural host.

View Article: PubMed Central - PubMed

Affiliation: Charité School of Medicine, Berlin, Germany.

ABSTRACT
Dobrava-Belgrade virus (DOBV) is a European hantavirus that causes hemorrhagic fever with renal syndrome (HFRS); case-fatality rates in Balkan countries are as high as 12%. To determine causative agents, we examined 126 cases of DOBV-associated HFRS in central and southern European Russia. In central Russia (Lipetsk, Voronezh, Orel regions), outbreaks were caused by a DOBV variant (DOBV-Aa) carried by Apodemus agrarius. In southern Russia (Sochi district), where HFRS is endemic, HFRS cases were caused by a new DOBV variant (DOBV-Ap), found in A. ponticus, a novel hantavirus natural host. Both viruses, DOBV-Aa/Lipetsk and DOBV-Ap/Sochi, were isolated through Vero E6 cells, genetically characterized, and used for serotyping of the HFRS patients' serum. The clinical severity of HFRS caused by DOBV-Aa resembles that of HFRS caused by Puumala virus (mild to moderate); clinical severity of disease caused by DOBV-Ap infections is more often moderate to severe.

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Phylogenetic analysis of D-loop sequences of the animal sources of the viruses Sochi/Ap and Lipetsk/Aa (in boldface): Apodemus ponticus from the Sochi region (Ap1584/Sochi; EU188455) and A. agrarius from Lipetsk region (Aa1854/Lipetsk; EU188456). Sequences of other Apodemus spp. were obtained from GenBank; accession numbers are indicated at the branch tips. The neighbor-joining tree was constructed by using the Tamura-Nei (TN93) evolutionary model. Values above the tree branches represent the bootstrap values calculated from 10,000 replicates. The scale bar indicates an evolutionary distance of 0.1 substitutions per position in the sequence.
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Figure 1: Phylogenetic analysis of D-loop sequences of the animal sources of the viruses Sochi/Ap and Lipetsk/Aa (in boldface): Apodemus ponticus from the Sochi region (Ap1584/Sochi; EU188455) and A. agrarius from Lipetsk region (Aa1854/Lipetsk; EU188456). Sequences of other Apodemus spp. were obtained from GenBank; accession numbers are indicated at the branch tips. The neighbor-joining tree was constructed by using the Tamura-Nei (TN93) evolutionary model. Values above the tree branches represent the bootstrap values calculated from 10,000 replicates. The scale bar indicates an evolutionary distance of 0.1 substitutions per position in the sequence.

Mentions: To ensure correct classification of the reservoir hosts, tissue samples of the 2 animals that served as the sources of virus isolation were subjected to DNA extraction and sequence analysis. Nucleotide sequence of the mitochondrial DNA fragment containing the control region, D-loop, was determined for both animals and compared with Apodemus spp. D-loop sequences from GenBank. Neighbor-joining phylogenetic analysis demonstrated that the Aa1854/Lipetsk animal was identified correctly as A. agrarius. However, no A. ponticus D-loop nucleotide sequence was available in GenBank for comparison. Nevertheless, phylogenetic analysis of Ap1584/Sochi showed that the obtained D-loop sequence was distinct from all other analyzed sequences (Figure 1). This finding at least confirms that Ap1584/Sochi was not a misidentified member of A. sylvaticus, A. flavicollis, or another morphologically similar Apodemus species.


Hemorrhagic fever with renal syndrome caused by 2 lineages of Dobrava hantavirus, Russia.

Klempa B, Tkachenko EA, Dzagurova TK, Yunicheva YV, Morozov VG, Okulova NM, Slyusareva GP, Smirnov A, Kruger DH - Emerging Infect. Dis. (2008)

Phylogenetic analysis of D-loop sequences of the animal sources of the viruses Sochi/Ap and Lipetsk/Aa (in boldface): Apodemus ponticus from the Sochi region (Ap1584/Sochi; EU188455) and A. agrarius from Lipetsk region (Aa1854/Lipetsk; EU188456). Sequences of other Apodemus spp. were obtained from GenBank; accession numbers are indicated at the branch tips. The neighbor-joining tree was constructed by using the Tamura-Nei (TN93) evolutionary model. Values above the tree branches represent the bootstrap values calculated from 10,000 replicates. The scale bar indicates an evolutionary distance of 0.1 substitutions per position in the sequence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570905&req=5

Figure 1: Phylogenetic analysis of D-loop sequences of the animal sources of the viruses Sochi/Ap and Lipetsk/Aa (in boldface): Apodemus ponticus from the Sochi region (Ap1584/Sochi; EU188455) and A. agrarius from Lipetsk region (Aa1854/Lipetsk; EU188456). Sequences of other Apodemus spp. were obtained from GenBank; accession numbers are indicated at the branch tips. The neighbor-joining tree was constructed by using the Tamura-Nei (TN93) evolutionary model. Values above the tree branches represent the bootstrap values calculated from 10,000 replicates. The scale bar indicates an evolutionary distance of 0.1 substitutions per position in the sequence.
Mentions: To ensure correct classification of the reservoir hosts, tissue samples of the 2 animals that served as the sources of virus isolation were subjected to DNA extraction and sequence analysis. Nucleotide sequence of the mitochondrial DNA fragment containing the control region, D-loop, was determined for both animals and compared with Apodemus spp. D-loop sequences from GenBank. Neighbor-joining phylogenetic analysis demonstrated that the Aa1854/Lipetsk animal was identified correctly as A. agrarius. However, no A. ponticus D-loop nucleotide sequence was available in GenBank for comparison. Nevertheless, phylogenetic analysis of Ap1584/Sochi showed that the obtained D-loop sequence was distinct from all other analyzed sequences (Figure 1). This finding at least confirms that Ap1584/Sochi was not a misidentified member of A. sylvaticus, A. flavicollis, or another morphologically similar Apodemus species.

Bottom Line: To determine causative agents, we examined 126 cases of DOBV-associated HFRS in central and southern European Russia.In central Russia (Lipetsk, Voronezh, Orel regions), outbreaks were caused by a DOBV variant (DOBV-Aa) carried by Apodemus agrarius.In southern Russia (Sochi district), where HFRS is endemic, HFRS cases were caused by a new DOBV variant (DOBV-Ap), found in A. ponticus, a novel hantavirus natural host.

View Article: PubMed Central - PubMed

Affiliation: Charité School of Medicine, Berlin, Germany.

ABSTRACT
Dobrava-Belgrade virus (DOBV) is a European hantavirus that causes hemorrhagic fever with renal syndrome (HFRS); case-fatality rates in Balkan countries are as high as 12%. To determine causative agents, we examined 126 cases of DOBV-associated HFRS in central and southern European Russia. In central Russia (Lipetsk, Voronezh, Orel regions), outbreaks were caused by a DOBV variant (DOBV-Aa) carried by Apodemus agrarius. In southern Russia (Sochi district), where HFRS is endemic, HFRS cases were caused by a new DOBV variant (DOBV-Ap), found in A. ponticus, a novel hantavirus natural host. Both viruses, DOBV-Aa/Lipetsk and DOBV-Ap/Sochi, were isolated through Vero E6 cells, genetically characterized, and used for serotyping of the HFRS patients' serum. The clinical severity of HFRS caused by DOBV-Aa resembles that of HFRS caused by Puumala virus (mild to moderate); clinical severity of disease caused by DOBV-Ap infections is more often moderate to severe.

Show MeSH
Related in: MedlinePlus