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Chikungunya fever in travelers returning to Europe from the Indian Ocean region, 2006.

Panning M, Grywna K, van Esbroeck M, Emmerich P, Drosten C - Emerging Infect. Dis. (2008)

Bottom Line: Reverse transcription-PCR was positive for all samples taken up to day 4 after symptom onset.Immunofluorescence detected immunoglobulin (Ig) M on day 1 and IgG on day 2 for some patients, and in all patients from day 5 onward.Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >10(9) copies/mL plasma).

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

ABSTRACT
Chikungunya fever has spread through several Indian Ocean islands and India, including popular travel destinations. To compare usefulness of diagnostic tests and to understand reasons for the magnitude and severity of an outbreak, we used 3 diagnostic methods to test 720 samples from 680 patients returning to Europe from the Indian Ocean region in 2006. Chikungunya infection was confirmed for 24.4% patients in the first half of the year and for 9.9% in the second half. Reverse transcription-PCR was positive for all samples taken up to day 4 after symptom onset. Immunofluorescence detected immunoglobulin (Ig) M on day 1 and IgG on day 2 for some patients, and in all patients from day 5 onward. Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >10(9) copies/mL plasma). These data will help healthcare providers select diagnostic tests for returning travelers.

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Related in: MedlinePlus

Immunoglobulin (Ig) M and IgG titers in 153 samples from 121 patients. Some patients are represented more than once if multiple specimens were submitted for testing. CHIKV, chikungunya virus.
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Figure 1: Immunoglobulin (Ig) M and IgG titers in 153 samples from 121 patients. Some patients are represented more than once if multiple specimens were submitted for testing. CHIKV, chikungunya virus.

Mentions: We were able to retrieve 153 samples, exact information on travel destination, onset of symptoms, and date of sampling for 121 patients (Figure 1). CHIKV IgG was found for 79% of patients (125 [81.7%] of 153 samples). The earliest sample with a detectable IgG titer had been drawn on day 2 after onset; the latest, on day 252. An isolated IgG titer, i.e., no concomitant IgM, was detected in 15 samples, 14 of which had been collected late after onset (median 86.5 days, range 39–252 days). The remaining sample, collected on day 2, was positive by RT-PCR.


Chikungunya fever in travelers returning to Europe from the Indian Ocean region, 2006.

Panning M, Grywna K, van Esbroeck M, Emmerich P, Drosten C - Emerging Infect. Dis. (2008)

Immunoglobulin (Ig) M and IgG titers in 153 samples from 121 patients. Some patients are represented more than once if multiple specimens were submitted for testing. CHIKV, chikungunya virus.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570846&req=5

Figure 1: Immunoglobulin (Ig) M and IgG titers in 153 samples from 121 patients. Some patients are represented more than once if multiple specimens were submitted for testing. CHIKV, chikungunya virus.
Mentions: We were able to retrieve 153 samples, exact information on travel destination, onset of symptoms, and date of sampling for 121 patients (Figure 1). CHIKV IgG was found for 79% of patients (125 [81.7%] of 153 samples). The earliest sample with a detectable IgG titer had been drawn on day 2 after onset; the latest, on day 252. An isolated IgG titer, i.e., no concomitant IgM, was detected in 15 samples, 14 of which had been collected late after onset (median 86.5 days, range 39–252 days). The remaining sample, collected on day 2, was positive by RT-PCR.

Bottom Line: Reverse transcription-PCR was positive for all samples taken up to day 4 after symptom onset.Immunofluorescence detected immunoglobulin (Ig) M on day 1 and IgG on day 2 for some patients, and in all patients from day 5 onward.Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >10(9) copies/mL plasma).

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

ABSTRACT
Chikungunya fever has spread through several Indian Ocean islands and India, including popular travel destinations. To compare usefulness of diagnostic tests and to understand reasons for the magnitude and severity of an outbreak, we used 3 diagnostic methods to test 720 samples from 680 patients returning to Europe from the Indian Ocean region in 2006. Chikungunya infection was confirmed for 24.4% patients in the first half of the year and for 9.9% in the second half. Reverse transcription-PCR was positive for all samples taken up to day 4 after symptom onset. Immunofluorescence detected immunoglobulin (Ig) M on day 1 and IgG on day 2 for some patients, and in all patients from day 5 onward. Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >10(9) copies/mL plasma). These data will help healthcare providers select diagnostic tests for returning travelers.

Show MeSH
Related in: MedlinePlus