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Sylvatic dengue virus type 2 activity in humans, Nigeria, 1966.

Vasilakis N, Tesh RB, Weaver SC - Emerging Infect. Dis. (2008)

Bottom Line: Using phylogenetic analysis of complete virus genomes from human isolates obtained in Nigeria in 1966, we identified sylvatic dengue virus (DENV) strains from 3 febrile patients.This finding extends current understanding of the role of sylvatic DENV in febrile disease and documents another focus of sylvatic DENV transmission in West Africa.

View Article: PubMed Central - PubMed

Affiliation: University of Texas Medical Branch, Galveston, Texas, USA.

ABSTRACT
Using phylogenetic analysis of complete virus genomes from human isolates obtained in Nigeria in 1966, we identified sylvatic dengue virus (DENV) strains from 3 febrile patients. This finding extends current understanding of the role of sylvatic DENV in febrile disease and documents another focus of sylvatic DENV transmission in West Africa.

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Phylogenetic relationships of 3 complete coding regions of Nigerian dengue virus type 2 (DENV-2) isolates obtained from febrile patients in Ibadan, Nigeria, during the 1966 rainy season. A total of 15 sylvatic DENV-2 genomes (shaded) were compared with human isolates of DENV-2 and representatives of DENV-1, DENV-3, and DENV-4. Phylogeny was inferred by using Bayesian analysis, and all horizontal branches are scaled according to the number of substitutions per site. Bootstrap values are shown for key nodes.
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Figure 1: Phylogenetic relationships of 3 complete coding regions of Nigerian dengue virus type 2 (DENV-2) isolates obtained from febrile patients in Ibadan, Nigeria, during the 1966 rainy season. A total of 15 sylvatic DENV-2 genomes (shaded) were compared with human isolates of DENV-2 and representatives of DENV-1, DENV-3, and DENV-4. Phylogeny was inferred by using Bayesian analysis, and all horizontal branches are scaled according to the number of substitutions per site. Bootstrap values are shown for key nodes.

Mentions: RNA was extracted from these 3 isolates, after passage in C6/36 mosquito cells, by using the QIAamp Viral RNA Mini Kit (QIAGEN, Valencia, CA, USA). Their complete genomic sequences were determined by designing overlapping PCR amplicons. After purification by electrophoresis on 1% agarose gels, both strands were sequenced directly by using the ABI (Roche Diagnostics, Indianapolis, IN, USA) protocols with both the PCR and internal primers to derive a consensus sequence. We then generated a phylogenetic tree, including 22 endemic DENV-2 isolates representing strains from diverse localities throughout the tropics and neotropics, as well as representative strains of DENV-1, DENV-4 (including sylvatic isolates P72–1244 and P75–215, respectively), and DENV-3 strains as outgroups (41 sequences, 10,185 nt in length). Phylogenetic trees were inferred by using Bayesian analysis with 1 million iterations (9) as well as maximum parsimony and neighbor-joining implemented in PAUP version 4.0 (10) (Figure).


Sylvatic dengue virus type 2 activity in humans, Nigeria, 1966.

Vasilakis N, Tesh RB, Weaver SC - Emerging Infect. Dis. (2008)

Phylogenetic relationships of 3 complete coding regions of Nigerian dengue virus type 2 (DENV-2) isolates obtained from febrile patients in Ibadan, Nigeria, during the 1966 rainy season. A total of 15 sylvatic DENV-2 genomes (shaded) were compared with human isolates of DENV-2 and representatives of DENV-1, DENV-3, and DENV-4. Phylogeny was inferred by using Bayesian analysis, and all horizontal branches are scaled according to the number of substitutions per site. Bootstrap values are shown for key nodes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570833&req=5

Figure 1: Phylogenetic relationships of 3 complete coding regions of Nigerian dengue virus type 2 (DENV-2) isolates obtained from febrile patients in Ibadan, Nigeria, during the 1966 rainy season. A total of 15 sylvatic DENV-2 genomes (shaded) were compared with human isolates of DENV-2 and representatives of DENV-1, DENV-3, and DENV-4. Phylogeny was inferred by using Bayesian analysis, and all horizontal branches are scaled according to the number of substitutions per site. Bootstrap values are shown for key nodes.
Mentions: RNA was extracted from these 3 isolates, after passage in C6/36 mosquito cells, by using the QIAamp Viral RNA Mini Kit (QIAGEN, Valencia, CA, USA). Their complete genomic sequences were determined by designing overlapping PCR amplicons. After purification by electrophoresis on 1% agarose gels, both strands were sequenced directly by using the ABI (Roche Diagnostics, Indianapolis, IN, USA) protocols with both the PCR and internal primers to derive a consensus sequence. We then generated a phylogenetic tree, including 22 endemic DENV-2 isolates representing strains from diverse localities throughout the tropics and neotropics, as well as representative strains of DENV-1, DENV-4 (including sylvatic isolates P72–1244 and P75–215, respectively), and DENV-3 strains as outgroups (41 sequences, 10,185 nt in length). Phylogenetic trees were inferred by using Bayesian analysis with 1 million iterations (9) as well as maximum parsimony and neighbor-joining implemented in PAUP version 4.0 (10) (Figure).

Bottom Line: Using phylogenetic analysis of complete virus genomes from human isolates obtained in Nigeria in 1966, we identified sylvatic dengue virus (DENV) strains from 3 febrile patients.This finding extends current understanding of the role of sylvatic DENV in febrile disease and documents another focus of sylvatic DENV transmission in West Africa.

View Article: PubMed Central - PubMed

Affiliation: University of Texas Medical Branch, Galveston, Texas, USA.

ABSTRACT
Using phylogenetic analysis of complete virus genomes from human isolates obtained in Nigeria in 1966, we identified sylvatic dengue virus (DENV) strains from 3 febrile patients. This finding extends current understanding of the role of sylvatic DENV in febrile disease and documents another focus of sylvatic DENV transmission in West Africa.

Show MeSH
Related in: MedlinePlus