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Highly pathogenic avian influenza virus (H5N1) in domestic poultry and relationship with migratory birds, South Korea.

Lee YJ, Choi YK, Kim YJ, Song MS, Jeong OM, Lee EK, Jeon WJ, Jeong W, Joh SJ, Choi KS, Her M, Kim MC, Kim A, Kim MJ, Ho Lee E, Oh TG, Moon HJ, Yoo DW, Kim JH, Sung MH, Poo H, Kwon JH, Kim CJ - Emerging Infect. Dis. (2008)

Bottom Line: During the 2006-2007 winter season in South Korea, several outbreaks of highly pathogenic avian influenza virus (H5N1) were confirmed among domestic poultry and in migratory bird habitats.Phylogenetic analysis showed that all isolates were closely related and that all belong to the A/bar-headed goose/Qinghai/5/2005-like lineage rather than the A/chicken/Korea/ES/2003-like lineage.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture and Forestry, Anyang, South Korea.

ABSTRACT
During the 2006-2007 winter season in South Korea, several outbreaks of highly pathogenic avian influenza virus (H5N1) were confirmed among domestic poultry and in migratory bird habitats. Phylogenetic analysis showed that all isolates were closely related and that all belong to the A/bar-headed goose/Qinghai/5/2005-like lineage rather than the A/chicken/Korea/ES/2003-like lineage.

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Related in: MedlinePlus

Phylogenetic trees for hemagglutinin (HA) genes of Korean influenza virus (H5N1) isolates from wild birds and poultry farms during 2006–2007. The DNA sequences were compiled and edited by using the Lasergene sequence analysis software package (DNASTAR, Madison, WI, USA). Multiple sequence alignments were made by using ClustalX (10). Rooted phylograms were prepared with the neighbor-joining algorithm and then plotted by using NJplot (11). Branch lengths are proportional to sequence divergence and can be measured relative to the scale bar shown (0.01-nt changes per site). Branch labels record the stability of the branches >1,000 bootstrap replicates. The tree was produced by referring to the proposed global nomenclature system for influenza virus (H5N1) (www.offlu.net). Boldface indicates isolates tested in the current study.
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Figure 2: Phylogenetic trees for hemagglutinin (HA) genes of Korean influenza virus (H5N1) isolates from wild birds and poultry farms during 2006–2007. The DNA sequences were compiled and edited by using the Lasergene sequence analysis software package (DNASTAR, Madison, WI, USA). Multiple sequence alignments were made by using ClustalX (10). Rooted phylograms were prepared with the neighbor-joining algorithm and then plotted by using NJplot (11). Branch lengths are proportional to sequence divergence and can be measured relative to the scale bar shown (0.01-nt changes per site). Branch labels record the stability of the branches >1,000 bootstrap replicates. The tree was produced by referring to the proposed global nomenclature system for influenza virus (H5N1) (www.offlu.net). Boldface indicates isolates tested in the current study.

Mentions: Our phylogenetic analysis of the hemagglutinin (HA) genes of all Korean isolates showed that the isolates belong to the A/bar-headed goose/QH/65/2005 (QH/2005)–like lineage that caused an outbreak among wild birds at Qinghai Lake in China during 2005, rather than the first HPAI (H5N1) lineage (A/chicken/Korea/ES/2003) that infected farms in Korea in 2003 (Figure 2). Notably, the 2 isolates from migratory bird habitats were closely related to the H5N1 subtype poultry virus strains: A/environment/Korea/W149/2006 was similar to the viruses that occurred in Chungcheongnam-Do, and A/environment/Korea/W150/2006 was similar to viruses that affected birds in Jeollabuk-Do. However, all H5N1 subtype virus strains have a series of basic amino acids at the HA cleavage site (PQGERRRKKR/G), which is a characteristic of influenza viruses that are highly pathogenic to chickens (4,5). The intravenous pathogenicity index score of A/chicken/Korea/IS/2006 was 3.0 in chickens.


Highly pathogenic avian influenza virus (H5N1) in domestic poultry and relationship with migratory birds, South Korea.

Lee YJ, Choi YK, Kim YJ, Song MS, Jeong OM, Lee EK, Jeon WJ, Jeong W, Joh SJ, Choi KS, Her M, Kim MC, Kim A, Kim MJ, Ho Lee E, Oh TG, Moon HJ, Yoo DW, Kim JH, Sung MH, Poo H, Kwon JH, Kim CJ - Emerging Infect. Dis. (2008)

Phylogenetic trees for hemagglutinin (HA) genes of Korean influenza virus (H5N1) isolates from wild birds and poultry farms during 2006–2007. The DNA sequences were compiled and edited by using the Lasergene sequence analysis software package (DNASTAR, Madison, WI, USA). Multiple sequence alignments were made by using ClustalX (10). Rooted phylograms were prepared with the neighbor-joining algorithm and then plotted by using NJplot (11). Branch lengths are proportional to sequence divergence and can be measured relative to the scale bar shown (0.01-nt changes per site). Branch labels record the stability of the branches >1,000 bootstrap replicates. The tree was produced by referring to the proposed global nomenclature system for influenza virus (H5N1) (www.offlu.net). Boldface indicates isolates tested in the current study.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570817&req=5

Figure 2: Phylogenetic trees for hemagglutinin (HA) genes of Korean influenza virus (H5N1) isolates from wild birds and poultry farms during 2006–2007. The DNA sequences were compiled and edited by using the Lasergene sequence analysis software package (DNASTAR, Madison, WI, USA). Multiple sequence alignments were made by using ClustalX (10). Rooted phylograms were prepared with the neighbor-joining algorithm and then plotted by using NJplot (11). Branch lengths are proportional to sequence divergence and can be measured relative to the scale bar shown (0.01-nt changes per site). Branch labels record the stability of the branches >1,000 bootstrap replicates. The tree was produced by referring to the proposed global nomenclature system for influenza virus (H5N1) (www.offlu.net). Boldface indicates isolates tested in the current study.
Mentions: Our phylogenetic analysis of the hemagglutinin (HA) genes of all Korean isolates showed that the isolates belong to the A/bar-headed goose/QH/65/2005 (QH/2005)–like lineage that caused an outbreak among wild birds at Qinghai Lake in China during 2005, rather than the first HPAI (H5N1) lineage (A/chicken/Korea/ES/2003) that infected farms in Korea in 2003 (Figure 2). Notably, the 2 isolates from migratory bird habitats were closely related to the H5N1 subtype poultry virus strains: A/environment/Korea/W149/2006 was similar to the viruses that occurred in Chungcheongnam-Do, and A/environment/Korea/W150/2006 was similar to viruses that affected birds in Jeollabuk-Do. However, all H5N1 subtype virus strains have a series of basic amino acids at the HA cleavage site (PQGERRRKKR/G), which is a characteristic of influenza viruses that are highly pathogenic to chickens (4,5). The intravenous pathogenicity index score of A/chicken/Korea/IS/2006 was 3.0 in chickens.

Bottom Line: During the 2006-2007 winter season in South Korea, several outbreaks of highly pathogenic avian influenza virus (H5N1) were confirmed among domestic poultry and in migratory bird habitats.Phylogenetic analysis showed that all isolates were closely related and that all belong to the A/bar-headed goose/Qinghai/5/2005-like lineage rather than the A/chicken/Korea/ES/2003-like lineage.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture and Forestry, Anyang, South Korea.

ABSTRACT
During the 2006-2007 winter season in South Korea, several outbreaks of highly pathogenic avian influenza virus (H5N1) were confirmed among domestic poultry and in migratory bird habitats. Phylogenetic analysis showed that all isolates were closely related and that all belong to the A/bar-headed goose/Qinghai/5/2005-like lineage rather than the A/chicken/Korea/ES/2003-like lineage.

Show MeSH
Related in: MedlinePlus