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Dolphin morbillivirus epizootic resurgence, Mediterranean Sea.

Raga JA, Banyard A, Domingo M, Corteyn M, Van Bressem MF, Fernández M, Aznar FJ, Barrett T - Emerging Infect. Dis. (2008)

Bottom Line: In July 2007, > 100 striped dolphins, Stenella coeruleoalba, were found dead along the coast of the Spanish Mediterranean.Of 10 dolphins tested, 7 were positive for a virus strain closely related to the dolphin morbillivirus that was isolated during a previous epizootic in 1990.

View Article: PubMed Central - PubMed

Affiliation: University of Valencia, Valencia, Spain.

ABSTRACT
In July 2007, > 100 striped dolphins, Stenella coeruleoalba, were found dead along the coast of the Spanish Mediterranean. Of 10 dolphins tested, 7 were positive for a virus strain closely related to the dolphin morbillivirus that was isolated during a previous epizootic in 1990.

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Related in: MedlinePlus

Evidence concerning the identity of the morbillivirus infecting Mediterranean striped dolphins in 2007. A) Immunohistologic staining of lung tissue with a MoAb anti CDV-NP, counterstained with hematoxylin. Epithelial bronchiolar cells and cells in the bronchiolar lumen are positively stained. B) Phylogenetic analysis of the N1/N2 region of the morbillivirus N genes. The number indicated at each node represents the bootstrap value after 10,000 replicates. The evolutionary distances were computed with the Kimura 2-parameter method and are in the units of the number of base substitutions per site. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Details of each isolate used for this study and accession numbers for each of the sequences are as follows: DMV 2007 isolate (EU124652), PMV G12 isolate (AY949833), PPRV Nigeria/75/1 Vaccine (X74443), PDV DK/88 strain (X75717), RPV RBOK vaccine strain (Z30697), DMV isolate (AJ608288), CDV Onderstepoort vaccine strain (AF305419), and MV Edmonston vaccine strain (AF266288). DMV, dolphin morbillivirus; PMV, porpoise morbillivirus; PPRV, peste des petits ruminants virus; PDV, phocine distemper virus; RPV RBOK, rinderpest virus RBOK strain; CDV, canine distemper virus; MV, measles virus.
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Figure 2: Evidence concerning the identity of the morbillivirus infecting Mediterranean striped dolphins in 2007. A) Immunohistologic staining of lung tissue with a MoAb anti CDV-NP, counterstained with hematoxylin. Epithelial bronchiolar cells and cells in the bronchiolar lumen are positively stained. B) Phylogenetic analysis of the N1/N2 region of the morbillivirus N genes. The number indicated at each node represents the bootstrap value after 10,000 replicates. The evolutionary distances were computed with the Kimura 2-parameter method and are in the units of the number of base substitutions per site. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Details of each isolate used for this study and accession numbers for each of the sequences are as follows: DMV 2007 isolate (EU124652), PMV G12 isolate (AY949833), PPRV Nigeria/75/1 Vaccine (X74443), PDV DK/88 strain (X75717), RPV RBOK vaccine strain (Z30697), DMV isolate (AJ608288), CDV Onderstepoort vaccine strain (AF305419), and MV Edmonston vaccine strain (AF266288). DMV, dolphin morbillivirus; PMV, porpoise morbillivirus; PPRV, peste des petits ruminants virus; PDV, phocine distemper virus; RPV RBOK, rinderpest virus RBOK strain; CDV, canine distemper virus; MV, measles virus.

Mentions: Lesions comprised multifocal bronchiolo-interstitial pneumonia with syncytial cells and nuclear inclusions in the alveolar epithelium and syncytia, as well as lymphoid depletion of the cortical area of the lymph nodes. In 1 dolphin, diffuse pyogranulomatous pneumonia of probable bacterial origin, with disseminated foci to many organs (e.g., brain, heart, lymph nodes, spleen), was found concomitantly with DMV infection. For 5 dolphins, immunostaining was observed in epithelial cells and syncytia in the lungs (Figure 2, panel A), lymphoid cells, neurons, and/or bile duct epithelium. The staining pattern was similar to that described for animals affected by the 1990 epizootic (8).


Dolphin morbillivirus epizootic resurgence, Mediterranean Sea.

Raga JA, Banyard A, Domingo M, Corteyn M, Van Bressem MF, Fernández M, Aznar FJ, Barrett T - Emerging Infect. Dis. (2008)

Evidence concerning the identity of the morbillivirus infecting Mediterranean striped dolphins in 2007. A) Immunohistologic staining of lung tissue with a MoAb anti CDV-NP, counterstained with hematoxylin. Epithelial bronchiolar cells and cells in the bronchiolar lumen are positively stained. B) Phylogenetic analysis of the N1/N2 region of the morbillivirus N genes. The number indicated at each node represents the bootstrap value after 10,000 replicates. The evolutionary distances were computed with the Kimura 2-parameter method and are in the units of the number of base substitutions per site. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Details of each isolate used for this study and accession numbers for each of the sequences are as follows: DMV 2007 isolate (EU124652), PMV G12 isolate (AY949833), PPRV Nigeria/75/1 Vaccine (X74443), PDV DK/88 strain (X75717), RPV RBOK vaccine strain (Z30697), DMV isolate (AJ608288), CDV Onderstepoort vaccine strain (AF305419), and MV Edmonston vaccine strain (AF266288). DMV, dolphin morbillivirus; PMV, porpoise morbillivirus; PPRV, peste des petits ruminants virus; PDV, phocine distemper virus; RPV RBOK, rinderpest virus RBOK strain; CDV, canine distemper virus; MV, measles virus.
© Copyright Policy
Related In: Results  -  Collection

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Figure 2: Evidence concerning the identity of the morbillivirus infecting Mediterranean striped dolphins in 2007. A) Immunohistologic staining of lung tissue with a MoAb anti CDV-NP, counterstained with hematoxylin. Epithelial bronchiolar cells and cells in the bronchiolar lumen are positively stained. B) Phylogenetic analysis of the N1/N2 region of the morbillivirus N genes. The number indicated at each node represents the bootstrap value after 10,000 replicates. The evolutionary distances were computed with the Kimura 2-parameter method and are in the units of the number of base substitutions per site. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Details of each isolate used for this study and accession numbers for each of the sequences are as follows: DMV 2007 isolate (EU124652), PMV G12 isolate (AY949833), PPRV Nigeria/75/1 Vaccine (X74443), PDV DK/88 strain (X75717), RPV RBOK vaccine strain (Z30697), DMV isolate (AJ608288), CDV Onderstepoort vaccine strain (AF305419), and MV Edmonston vaccine strain (AF266288). DMV, dolphin morbillivirus; PMV, porpoise morbillivirus; PPRV, peste des petits ruminants virus; PDV, phocine distemper virus; RPV RBOK, rinderpest virus RBOK strain; CDV, canine distemper virus; MV, measles virus.
Mentions: Lesions comprised multifocal bronchiolo-interstitial pneumonia with syncytial cells and nuclear inclusions in the alveolar epithelium and syncytia, as well as lymphoid depletion of the cortical area of the lymph nodes. In 1 dolphin, diffuse pyogranulomatous pneumonia of probable bacterial origin, with disseminated foci to many organs (e.g., brain, heart, lymph nodes, spleen), was found concomitantly with DMV infection. For 5 dolphins, immunostaining was observed in epithelial cells and syncytia in the lungs (Figure 2, panel A), lymphoid cells, neurons, and/or bile duct epithelium. The staining pattern was similar to that described for animals affected by the 1990 epizootic (8).

Bottom Line: In July 2007, > 100 striped dolphins, Stenella coeruleoalba, were found dead along the coast of the Spanish Mediterranean.Of 10 dolphins tested, 7 were positive for a virus strain closely related to the dolphin morbillivirus that was isolated during a previous epizootic in 1990.

View Article: PubMed Central - PubMed

Affiliation: University of Valencia, Valencia, Spain.

ABSTRACT
In July 2007, > 100 striped dolphins, Stenella coeruleoalba, were found dead along the coast of the Spanish Mediterranean. Of 10 dolphins tested, 7 were positive for a virus strain closely related to the dolphin morbillivirus that was isolated during a previous epizootic in 1990.

Show MeSH
Related in: MedlinePlus