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Novel relapsing fever spirochete in bat tick.

Gill JS, Ullmann AJ, Loftis AD, Schwan TG, Raffel SJ, Schrumpf ME, Piesman J - Emerging Infect. Dis. (2008)

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Tick-borne relapsing fever in western North America is a zoonosis caused by spirochetes in the genus Borrelia that are transmitted by argasid ticks of the genus Ornithodoros... Subsequently, Loftis et al. used PCR analysis and DNA sequencing to detect in C. kelleyi an unidentified Borrelia species that was closely related to B. turicatae and B. parkeri... We report the partial molecular characterization of another novel tick-borne relapsing fever spirochete in C. kelleyi, which expands our knowledge for this group of pathogenic spirochetes and their potential vertebrate hosts and tick vectors... The Borrelia glpQ sequence from tick 3 had 85.1%–89.1% identity compared with glpQ sequences from B. hermsii, B. turicatae, and B. parkeri... This finding suggests the presence of at least 2 relapsing fever group spirochetes in C. kelleyi that await further characterization... We found a novel Borrelia in bat ticks that is closely related to, but distinct from, the other known species of tick-borne relapsing fever spirochetes in North America... The human health implications of the new relapsing fever group spirochete are not yet known... The willingness of C. kelleyi to feed on humans and the fact that infection with bacteria closely related to true relapsing fever spirochetes occurs in these ticks suggest that human habitation near bats and their associated tick colonies could pose a public health risk... Growth in laboratory animals or culture could help isolate these novel organisms for further studies to establish the distribution and public health implications of this newly identified Borrelia sp.

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Phylogram comparing the novel spirochete in the bat tick Carios kelleyi with Borrelia parkeri, B. turicatae, and B. hermsii based on the concatenated partial 16S rRNA-flaB-glpQ DNA sequences in the Carios spirochete (1,992 bp total) (produced with ClustalV software from DNASTAR [Madison, WI, USA]). Scale bar represents the number of base substitutions per 100 aligned bases. GenBank accession numbers for the C. kelleyi spirochete sequences used to construct the tree are EF688575, EF688576, and EF688577. Spiro, spirochete.
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Figure 1: Phylogram comparing the novel spirochete in the bat tick Carios kelleyi with Borrelia parkeri, B. turicatae, and B. hermsii based on the concatenated partial 16S rRNA-flaB-glpQ DNA sequences in the Carios spirochete (1,992 bp total) (produced with ClustalV software from DNASTAR [Madison, WI, USA]). Scale bar represents the number of base substitutions per 100 aligned bases. GenBank accession numbers for the C. kelleyi spirochete sequences used to construct the tree are EF688575, EF688576, and EF688577. Spiro, spirochete.

Mentions: Fourteen (45.1%) of 31 ticks were positive by PCR for >1 of the genes tested. Partial DNA sequences were determined from tick no. 16, for which amplicons for all 3 genes were obtained. The partial flaB sequence had 4 bases different from the 300-base sequence (98.66% identity) reported previously (GenBank accession no. AY763104) for another Borrelia sp. found in C. kelleyi (5). We constructed a 1,992-bp concatenated sequence that contained 1,273 bp of the 16S rRNA, 351 bp of flaB, and 368 bp of glpQ. This concatenated sequence was aligned with homologous, trimmed DNA sequences of the same length obtained from representative full-length sequences determined previously for B. hermsii, B. turicatae, and B. parkeri (3,9) (Figure). This C. kelleyi spirochete was more closely related to B. turicatae and B. parkeri than to B. hermsii but was clearly distinct from all 3 species (DNA sequence identities of 98.89%, 98.75%, and 95.98% to B. turicatae, B. parkeri, and B. hermsii, respectively).


Novel relapsing fever spirochete in bat tick.

Gill JS, Ullmann AJ, Loftis AD, Schwan TG, Raffel SJ, Schrumpf ME, Piesman J - Emerging Infect. Dis. (2008)

Phylogram comparing the novel spirochete in the bat tick Carios kelleyi with Borrelia parkeri, B. turicatae, and B. hermsii based on the concatenated partial 16S rRNA-flaB-glpQ DNA sequences in the Carios spirochete (1,992 bp total) (produced with ClustalV software from DNASTAR [Madison, WI, USA]). Scale bar represents the number of base substitutions per 100 aligned bases. GenBank accession numbers for the C. kelleyi spirochete sequences used to construct the tree are EF688575, EF688576, and EF688577. Spiro, spirochete.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2570806&req=5

Figure 1: Phylogram comparing the novel spirochete in the bat tick Carios kelleyi with Borrelia parkeri, B. turicatae, and B. hermsii based on the concatenated partial 16S rRNA-flaB-glpQ DNA sequences in the Carios spirochete (1,992 bp total) (produced with ClustalV software from DNASTAR [Madison, WI, USA]). Scale bar represents the number of base substitutions per 100 aligned bases. GenBank accession numbers for the C. kelleyi spirochete sequences used to construct the tree are EF688575, EF688576, and EF688577. Spiro, spirochete.
Mentions: Fourteen (45.1%) of 31 ticks were positive by PCR for >1 of the genes tested. Partial DNA sequences were determined from tick no. 16, for which amplicons for all 3 genes were obtained. The partial flaB sequence had 4 bases different from the 300-base sequence (98.66% identity) reported previously (GenBank accession no. AY763104) for another Borrelia sp. found in C. kelleyi (5). We constructed a 1,992-bp concatenated sequence that contained 1,273 bp of the 16S rRNA, 351 bp of flaB, and 368 bp of glpQ. This concatenated sequence was aligned with homologous, trimmed DNA sequences of the same length obtained from representative full-length sequences determined previously for B. hermsii, B. turicatae, and B. parkeri (3,9) (Figure). This C. kelleyi spirochete was more closely related to B. turicatae and B. parkeri than to B. hermsii but was clearly distinct from all 3 species (DNA sequence identities of 98.89%, 98.75%, and 95.98% to B. turicatae, B. parkeri, and B. hermsii, respectively).

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Tick-borne relapsing fever in western North America is a zoonosis caused by spirochetes in the genus Borrelia that are transmitted by argasid ticks of the genus Ornithodoros... Subsequently, Loftis et al. used PCR analysis and DNA sequencing to detect in C. kelleyi an unidentified Borrelia species that was closely related to B. turicatae and B. parkeri... We report the partial molecular characterization of another novel tick-borne relapsing fever spirochete in C. kelleyi, which expands our knowledge for this group of pathogenic spirochetes and their potential vertebrate hosts and tick vectors... The Borrelia glpQ sequence from tick 3 had 85.1%–89.1% identity compared with glpQ sequences from B. hermsii, B. turicatae, and B. parkeri... This finding suggests the presence of at least 2 relapsing fever group spirochetes in C. kelleyi that await further characterization... We found a novel Borrelia in bat ticks that is closely related to, but distinct from, the other known species of tick-borne relapsing fever spirochetes in North America... The human health implications of the new relapsing fever group spirochete are not yet known... The willingness of C. kelleyi to feed on humans and the fact that infection with bacteria closely related to true relapsing fever spirochetes occurs in these ticks suggest that human habitation near bats and their associated tick colonies could pose a public health risk... Growth in laboratory animals or culture could help isolate these novel organisms for further studies to establish the distribution and public health implications of this newly identified Borrelia sp.

Show MeSH
Related in: MedlinePlus