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Hemagglutinating encephalomyelitis coronavirus infection in pigs, Argentina.

Quiroga MA, Cappuccio J, Piñeyro P, Basso W, Moré G, Kienast M, Schonfeld S, Cáncer JL, Arauz S, Pintos ME, Nanni M, Machuca M, Hirano N, Perfumo CJ - Emerging Infect. Dis. (2008)

Bottom Line: We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in Argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus (PHE-CoV) infection.Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcription-PCR, and genomic sequencing.This study documents PHE-CoV infection in South America.

View Article: PubMed Central - PubMed

Affiliation: Universidad Nacional de la Plata, La Plata, Argentina. mquiroga@fcv.unlp.edu.ar

ABSTRACT
We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in Argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus (PHE-CoV) infection. Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcription-PCR, and genomic sequencing. This study documents PHE-CoV infection in South America.

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Related in: MedlinePlus

Polyacrylamide gel and silver staining of reverse transcription–PCR products from brains of piglets infected with porcine hemagglutinating encephalomyelitis coronavirus. Amplicons of ≈250 bp were found in brain samples from pigs 6, 8, 9, and 11 days of age. Neg, negative control (water + mastermix); PK15, amplification of PK15 cells inoculated with brain and tonsil from affected piglet; AP, asymptomatic piglet; and Ladder, 50-bp Fermentas.
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Figure 2: Polyacrylamide gel and silver staining of reverse transcription–PCR products from brains of piglets infected with porcine hemagglutinating encephalomyelitis coronavirus. Amplicons of ≈250 bp were found in brain samples from pigs 6, 8, 9, and 11 days of age. Neg, negative control (water + mastermix); PK15, amplification of PK15 cells inoculated with brain and tonsil from affected piglet; AP, asymptomatic piglet; and Ladder, 50-bp Fermentas.

Mentions: RT-PCR analysis showed a product of the expected size for CoV (≈250 bp) in all analyzed brain samples. No amplification was observed in inoculated PK 15 cells (Figure 2). The relationship between clinical course, lesions, and IHC and RT-PCR results is shown in the Table. A constant 116-nt sequence was obtained for all products amplified from symptomatic piglets and submitted to GenBank (accession no. EF602436). The sequence showed a 95% identity with the complete genome of PHE-CoV strain VW572 (accession no. DQ 011855) and with PHE-CoV RNA-directed RNA polymerase gene (accession no. AF 124988). Detection of amplicons of ≈250 bp with “pancoronavirus” primers in brain samples is highly suggestive of the presence of PHE-CoV. Sequence analysis confirmed this observation. Cytopathic effects were not observed, and PHE-CoV antigens were not detected in inoculated cells.


Hemagglutinating encephalomyelitis coronavirus infection in pigs, Argentina.

Quiroga MA, Cappuccio J, Piñeyro P, Basso W, Moré G, Kienast M, Schonfeld S, Cáncer JL, Arauz S, Pintos ME, Nanni M, Machuca M, Hirano N, Perfumo CJ - Emerging Infect. Dis. (2008)

Polyacrylamide gel and silver staining of reverse transcription–PCR products from brains of piglets infected with porcine hemagglutinating encephalomyelitis coronavirus. Amplicons of ≈250 bp were found in brain samples from pigs 6, 8, 9, and 11 days of age. Neg, negative control (water + mastermix); PK15, amplification of PK15 cells inoculated with brain and tonsil from affected piglet; AP, asymptomatic piglet; and Ladder, 50-bp Fermentas.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570804&req=5

Figure 2: Polyacrylamide gel and silver staining of reverse transcription–PCR products from brains of piglets infected with porcine hemagglutinating encephalomyelitis coronavirus. Amplicons of ≈250 bp were found in brain samples from pigs 6, 8, 9, and 11 days of age. Neg, negative control (water + mastermix); PK15, amplification of PK15 cells inoculated with brain and tonsil from affected piglet; AP, asymptomatic piglet; and Ladder, 50-bp Fermentas.
Mentions: RT-PCR analysis showed a product of the expected size for CoV (≈250 bp) in all analyzed brain samples. No amplification was observed in inoculated PK 15 cells (Figure 2). The relationship between clinical course, lesions, and IHC and RT-PCR results is shown in the Table. A constant 116-nt sequence was obtained for all products amplified from symptomatic piglets and submitted to GenBank (accession no. EF602436). The sequence showed a 95% identity with the complete genome of PHE-CoV strain VW572 (accession no. DQ 011855) and with PHE-CoV RNA-directed RNA polymerase gene (accession no. AF 124988). Detection of amplicons of ≈250 bp with “pancoronavirus” primers in brain samples is highly suggestive of the presence of PHE-CoV. Sequence analysis confirmed this observation. Cytopathic effects were not observed, and PHE-CoV antigens were not detected in inoculated cells.

Bottom Line: We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in Argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus (PHE-CoV) infection.Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcription-PCR, and genomic sequencing.This study documents PHE-CoV infection in South America.

View Article: PubMed Central - PubMed

Affiliation: Universidad Nacional de la Plata, La Plata, Argentina. mquiroga@fcv.unlp.edu.ar

ABSTRACT
We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in Argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus (PHE-CoV) infection. Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcription-PCR, and genomic sequencing. This study documents PHE-CoV infection in South America.

Show MeSH
Related in: MedlinePlus