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Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans.

van Belkum A, Melles DC, Peeters JK, van Leeuwen WB, van Duijkeren E, Huijsdens XW, Spalburg E, de Neeling AJ, Verbrugh HA, Dutch Working Party on Surveillance and Research of MRSA-S - Emerging Infect. Dis. (2008)

Bottom Line: Methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers.ST398 methicillin-susceptible S. aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026).Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans.

View Article: PubMed Central - PubMed

Affiliation: University Medical Center Rotterdam, Rotterdam, the Netherlands.

ABSTRACT
Methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers. ST398 methicillin-susceptible S. aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026). Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans.

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Related in: MedlinePlus

Principal component analysis analysis of the amplified fragment length polymorphism data obtained for the pig-associated methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) and its closely related methicillin-susceptible S. aureus (MSSA) strains, carriage MSSA isolates from healthy children and elderly persons, invasive MSSA from hospitalized children and elderly persons, and invasive animal S. aureus isolates (including 2 MRSA isolates). The cubes, plotted in 3-dimensional space, represent all of the strains displayed in Figure 1, panel A. Each axis represents the score calculated for that strain on each principal component. The distribution is shown from 2 different angles. ST398 strains are circled. Blue, carriage isolates (n = 829); black, bacteremia isolates (n = 146); yellow, animal isolates (n = 77); red, ST398 MRSA isolates (n = 46); pink, reference strains (Mu50/N315).
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Figure 2: Principal component analysis analysis of the amplified fragment length polymorphism data obtained for the pig-associated methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) and its closely related methicillin-susceptible S. aureus (MSSA) strains, carriage MSSA isolates from healthy children and elderly persons, invasive MSSA from hospitalized children and elderly persons, and invasive animal S. aureus isolates (including 2 MRSA isolates). The cubes, plotted in 3-dimensional space, represent all of the strains displayed in Figure 1, panel A. Each axis represents the score calculated for that strain on each principal component. The distribution is shown from 2 different angles. ST398 strains are circled. Blue, carriage isolates (n = 829); black, bacteremia isolates (n = 146); yellow, animal isolates (n = 77); red, ST398 MRSA isolates (n = 46); pink, reference strains (Mu50/N315).

Mentions: After principle component analysis , the ST398 MRSA strains still clustered as a separate group (Figure 2). The AFLP analysis did not distinguish strains from pigs or pig farmers, and only a limited number of polymorphic AFLP fragments were seen. AFLP markers that were positive for the ST398 MRSAs and absent from the other strains, or vice versa, were sequenced. Of 30 fragments analyzed, 9 were ≈100% specific for the pig-associated strains. Another 3 fragments were present in a subset of the pig-associated strains only. Of these 12 fragments, 4 were not homologous with current entries in the GenBank database, including the 10 S. aureus full-genome sequences. Of the 12 pig-specific markers, 8 were homologous with known sequences, which suggests that these markers become pig-specific by point mutations in the AFLP primer annealing site(s) rather than by genomic rearrangement. Several of the sequences encode factors were associated with membranes or transport.


Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans.

van Belkum A, Melles DC, Peeters JK, van Leeuwen WB, van Duijkeren E, Huijsdens XW, Spalburg E, de Neeling AJ, Verbrugh HA, Dutch Working Party on Surveillance and Research of MRSA-S - Emerging Infect. Dis. (2008)

Principal component analysis analysis of the amplified fragment length polymorphism data obtained for the pig-associated methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) and its closely related methicillin-susceptible S. aureus (MSSA) strains, carriage MSSA isolates from healthy children and elderly persons, invasive MSSA from hospitalized children and elderly persons, and invasive animal S. aureus isolates (including 2 MRSA isolates). The cubes, plotted in 3-dimensional space, represent all of the strains displayed in Figure 1, panel A. Each axis represents the score calculated for that strain on each principal component. The distribution is shown from 2 different angles. ST398 strains are circled. Blue, carriage isolates (n = 829); black, bacteremia isolates (n = 146); yellow, animal isolates (n = 77); red, ST398 MRSA isolates (n = 46); pink, reference strains (Mu50/N315).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570802&req=5

Figure 2: Principal component analysis analysis of the amplified fragment length polymorphism data obtained for the pig-associated methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) and its closely related methicillin-susceptible S. aureus (MSSA) strains, carriage MSSA isolates from healthy children and elderly persons, invasive MSSA from hospitalized children and elderly persons, and invasive animal S. aureus isolates (including 2 MRSA isolates). The cubes, plotted in 3-dimensional space, represent all of the strains displayed in Figure 1, panel A. Each axis represents the score calculated for that strain on each principal component. The distribution is shown from 2 different angles. ST398 strains are circled. Blue, carriage isolates (n = 829); black, bacteremia isolates (n = 146); yellow, animal isolates (n = 77); red, ST398 MRSA isolates (n = 46); pink, reference strains (Mu50/N315).
Mentions: After principle component analysis , the ST398 MRSA strains still clustered as a separate group (Figure 2). The AFLP analysis did not distinguish strains from pigs or pig farmers, and only a limited number of polymorphic AFLP fragments were seen. AFLP markers that were positive for the ST398 MRSAs and absent from the other strains, or vice versa, were sequenced. Of 30 fragments analyzed, 9 were ≈100% specific for the pig-associated strains. Another 3 fragments were present in a subset of the pig-associated strains only. Of these 12 fragments, 4 were not homologous with current entries in the GenBank database, including the 10 S. aureus full-genome sequences. Of the 12 pig-specific markers, 8 were homologous with known sequences, which suggests that these markers become pig-specific by point mutations in the AFLP primer annealing site(s) rather than by genomic rearrangement. Several of the sequences encode factors were associated with membranes or transport.

Bottom Line: Methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers.ST398 methicillin-susceptible S. aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026).Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans.

View Article: PubMed Central - PubMed

Affiliation: University Medical Center Rotterdam, Rotterdam, the Netherlands.

ABSTRACT
Methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers. ST398 methicillin-susceptible S. aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026). Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans.

Show MeSH
Related in: MedlinePlus