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Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture.

Hattori K, Uematsu K, Matsumori H, Dohi Y, Takakura Y, Ohgushi H - BMC Res Notes (2008)

Bottom Line: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes.In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation.Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan. hattori@naramed-u.ac.jp

ABSTRACT

Background: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes. Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified.

Findings: We investigated the use of a spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution) to describe and quantify articular cartilage. In this study, we measured the colors of intact and repaired cartilage after a microfracture. Histologically, the repaired cartilage was a mixture of fibrocartilage and hyaline cartilage. In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation. Regarding the spectral reflectance distribution at 12 weeks after the operation, the repaired cartilage had a higher spectral reflectance ratio than intact cartilage between wavelengths of 400 to 470 nm.

Conclusion: This study reports the first results regarding the relationship between spectrocolorimetric evaluation and the histological findings of repair cartilage after a microfracture. Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

No MeSH data available.


Related in: MedlinePlus

Biochemical results. Bar graphs representing the cartilage constitutions in repaired rabbit cartilage. The (A) water contents, (B) hydroxyproline contents and (C) chondroitin sulfate contents of the three groups are shown. *P < 0.05 by the non-parametric Mann-Whitney U-test.
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Figure 5: Biochemical results. Bar graphs representing the cartilage constitutions in repaired rabbit cartilage. The (A) water contents, (B) hydroxyproline contents and (C) chondroitin sulfate contents of the three groups are shown. *P < 0.05 by the non-parametric Mann-Whitney U-test.

Mentions: The mean water contents (mean ± standard deviation) were 84.6 ± 8.9% in group M-2, 75.8 ± 6.1% in group M-4 and 57.5 ± 9.5% in group M-12 (Figure 5A). Significant differences in the water contents were found between groups M-2 and M-12 (P = 0.007) and between groups M-4 and M-12 (P = 0.004). The mean hydroxyproline contents (mean ± standard deviation) were 19.6 ± 8.9 nmol/mg in group M-2, 29.3 ± 7.4 nmol/mg in group M-4 and 27.6 ± 12.6 nmol/mg in group M-12 (Figure 5B). There were no significant differences among the three groups. The mean chondroitin sulfate contents (mean ± standard deviation) were 16.7 ± 8.1 nmol/mg in group M-2, 44.2 ± 25.6 nmol/mg in group M-4 and 18.1 ± 16.0 nmol/mg in group M-12 (Figure 5C). Significant differences in the chondroitin sulfate contents were found between groups M-2 and M-4 (P = 0.02) and between groups M-4 and M-12 (P = 0.04).


Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture.

Hattori K, Uematsu K, Matsumori H, Dohi Y, Takakura Y, Ohgushi H - BMC Res Notes (2008)

Biochemical results. Bar graphs representing the cartilage constitutions in repaired rabbit cartilage. The (A) water contents, (B) hydroxyproline contents and (C) chondroitin sulfate contents of the three groups are shown. *P < 0.05 by the non-parametric Mann-Whitney U-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570674&req=5

Figure 5: Biochemical results. Bar graphs representing the cartilage constitutions in repaired rabbit cartilage. The (A) water contents, (B) hydroxyproline contents and (C) chondroitin sulfate contents of the three groups are shown. *P < 0.05 by the non-parametric Mann-Whitney U-test.
Mentions: The mean water contents (mean ± standard deviation) were 84.6 ± 8.9% in group M-2, 75.8 ± 6.1% in group M-4 and 57.5 ± 9.5% in group M-12 (Figure 5A). Significant differences in the water contents were found between groups M-2 and M-12 (P = 0.007) and between groups M-4 and M-12 (P = 0.004). The mean hydroxyproline contents (mean ± standard deviation) were 19.6 ± 8.9 nmol/mg in group M-2, 29.3 ± 7.4 nmol/mg in group M-4 and 27.6 ± 12.6 nmol/mg in group M-12 (Figure 5B). There were no significant differences among the three groups. The mean chondroitin sulfate contents (mean ± standard deviation) were 16.7 ± 8.1 nmol/mg in group M-2, 44.2 ± 25.6 nmol/mg in group M-4 and 18.1 ± 16.0 nmol/mg in group M-12 (Figure 5C). Significant differences in the chondroitin sulfate contents were found between groups M-2 and M-4 (P = 0.02) and between groups M-4 and M-12 (P = 0.04).

Bottom Line: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes.In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation.Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan. hattori@naramed-u.ac.jp

ABSTRACT

Background: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes. Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified.

Findings: We investigated the use of a spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution) to describe and quantify articular cartilage. In this study, we measured the colors of intact and repaired cartilage after a microfracture. Histologically, the repaired cartilage was a mixture of fibrocartilage and hyaline cartilage. In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation. Regarding the spectral reflectance distribution at 12 weeks after the operation, the repaired cartilage had a higher spectral reflectance ratio than intact cartilage between wavelengths of 400 to 470 nm.

Conclusion: This study reports the first results regarding the relationship between spectrocolorimetric evaluation and the histological findings of repair cartilage after a microfracture. Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

No MeSH data available.


Related in: MedlinePlus