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Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture.

Hattori K, Uematsu K, Matsumori H, Dohi Y, Takakura Y, Ohgushi H - BMC Res Notes (2008)

Bottom Line: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes.In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation.Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan. hattori@naramed-u.ac.jp

ABSTRACT

Background: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes. Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified.

Findings: We investigated the use of a spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution) to describe and quantify articular cartilage. In this study, we measured the colors of intact and repaired cartilage after a microfracture. Histologically, the repaired cartilage was a mixture of fibrocartilage and hyaline cartilage. In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation. Regarding the spectral reflectance distribution at 12 weeks after the operation, the repaired cartilage had a higher spectral reflectance ratio than intact cartilage between wavelengths of 400 to 470 nm.

Conclusion: This study reports the first results regarding the relationship between spectrocolorimetric evaluation and the histological findings of repair cartilage after a microfracture. Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

No MeSH data available.


Related in: MedlinePlus

The spectral reflectance percentages (SRPs) of the three groups. SRP is as a coincidence index of the spectral reflectance distribution of the repaired cartilage with respect to intact cartilage. *P < 0.05 by the non-parametric Mann-Whitney U-test.
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Figure 3: The spectral reflectance percentages (SRPs) of the three groups. SRP is as a coincidence index of the spectral reflectance distribution of the repaired cartilage with respect to intact cartilage. *P < 0.05 by the non-parametric Mann-Whitney U-test.

Mentions: Typical examples of the spectral reflectance curves for groups C, M-2, M-4 and M-12 are shown in Figure 2. The spectral curves of all the groups showed two dips at 420 and 560 nm and a specific peak around 490 nm. There was a gradual increase in the spectral reflectance ratio from 620 to 700 nm. Across all the measured wavelengths, there was a low reflectance ratio in group M-2 compared with group C and a gradual increase in the reflectance ratio over the time course after the operation. As a characteristic difference, group M-12 had a higher spectral reflectance ratio than group C between 400 to 470 nm. The SRP values (mean ± standard deviation) as a coincidence index of the spectral reflectance curves were 47.5 ± 8.0% in group M-2, 59.4 ± 22.3% in group M-4 and 89.5 ± 14.4% in group M-12 (Figure 3). There were significant differences in the SRP values between groups M-2 and M-12 (P = 0.004) and between groups M-4 and M-12 (P = 0.01).


Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture.

Hattori K, Uematsu K, Matsumori H, Dohi Y, Takakura Y, Ohgushi H - BMC Res Notes (2008)

The spectral reflectance percentages (SRPs) of the three groups. SRP is as a coincidence index of the spectral reflectance distribution of the repaired cartilage with respect to intact cartilage. *P < 0.05 by the non-parametric Mann-Whitney U-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570674&req=5

Figure 3: The spectral reflectance percentages (SRPs) of the three groups. SRP is as a coincidence index of the spectral reflectance distribution of the repaired cartilage with respect to intact cartilage. *P < 0.05 by the non-parametric Mann-Whitney U-test.
Mentions: Typical examples of the spectral reflectance curves for groups C, M-2, M-4 and M-12 are shown in Figure 2. The spectral curves of all the groups showed two dips at 420 and 560 nm and a specific peak around 490 nm. There was a gradual increase in the spectral reflectance ratio from 620 to 700 nm. Across all the measured wavelengths, there was a low reflectance ratio in group M-2 compared with group C and a gradual increase in the reflectance ratio over the time course after the operation. As a characteristic difference, group M-12 had a higher spectral reflectance ratio than group C between 400 to 470 nm. The SRP values (mean ± standard deviation) as a coincidence index of the spectral reflectance curves were 47.5 ± 8.0% in group M-2, 59.4 ± 22.3% in group M-4 and 89.5 ± 14.4% in group M-12 (Figure 3). There were significant differences in the SRP values between groups M-2 and M-12 (P = 0.004) and between groups M-4 and M-12 (P = 0.01).

Bottom Line: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes.In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation.Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan. hattori@naramed-u.ac.jp

ABSTRACT

Background: In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes. Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified.

Findings: We investigated the use of a spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution) to describe and quantify articular cartilage. In this study, we measured the colors of intact and repaired cartilage after a microfracture. Histologically, the repaired cartilage was a mixture of fibrocartilage and hyaline cartilage. In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation. Regarding the spectral reflectance distribution at 12 weeks after the operation, the repaired cartilage had a higher spectral reflectance ratio than intact cartilage between wavelengths of 400 to 470 nm.

Conclusion: This study reports the first results regarding the relationship between spectrocolorimetric evaluation and the histological findings of repair cartilage after a microfracture. Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

No MeSH data available.


Related in: MedlinePlus