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Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


Related in: MedlinePlus

The phase shift in the absorption anisotropy due to a sample rotation (the same tendon section as in Figure 3 now oriented at ~60°).
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Figure 5: The phase shift in the absorption anisotropy due to a sample rotation (the same tendon section as in Figure 3 now oriented at ~60°).

Mentions: Another noticeable feature in Figure 4 is the 'phase shift' in the minimum and maximum absorption locations (angles) for a sample that is not oriented parallel/perpendicular with respect to the analyzer 0°. Though it appears like a full cycle in 0–180° angular space, the difference between the minimum and maximum absorption will always be 90°. To verify this observation, the regular section of the tendon was imaged when the section was tilted by about ~60° with respect to the initial orientation used in Figure 3. The results are shown in Figure 5, where both profiles of the amide anisotropy from this regular section show the 'phase shift'. (i.e., the amide I plot in Figure 5 is 'phase shifted' from the amide I plot in Figure 3a.) This anisotropy shift illustrates the importance of the specimen orientation in the FTIRI anisotropy experiment, as the anisotropy is a polarization dependent phenomenon.


Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

The phase shift in the absorption anisotropy due to a sample rotation (the same tendon section as in Figure 3 now oriented at ~60°).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570663&req=5

Figure 5: The phase shift in the absorption anisotropy due to a sample rotation (the same tendon section as in Figure 3 now oriented at ~60°).
Mentions: Another noticeable feature in Figure 4 is the 'phase shift' in the minimum and maximum absorption locations (angles) for a sample that is not oriented parallel/perpendicular with respect to the analyzer 0°. Though it appears like a full cycle in 0–180° angular space, the difference between the minimum and maximum absorption will always be 90°. To verify this observation, the regular section of the tendon was imaged when the section was tilted by about ~60° with respect to the initial orientation used in Figure 3. The results are shown in Figure 5, where both profiles of the amide anisotropy from this regular section show the 'phase shift'. (i.e., the amide I plot in Figure 5 is 'phase shifted' from the amide I plot in Figure 3a.) This anisotropy shift illustrates the importance of the specimen orientation in the FTIRI anisotropy experiment, as the anisotropy is a polarization dependent phenomenon.

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


Related in: MedlinePlus