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Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


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Absorption anisotropy of amide I (a) and amide II (b) of tendon in the regular, parallel and cross sections.
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Figure 3: Absorption anisotropy of amide I (a) and amide II (b) of tendon in the regular, parallel and cross sections.

Mentions: Figure 3 depicts the absorption anisotropy of amide I and amide II in tendon's regular, parallel and cross sections. Two features can be observed. First, the absorption anisotropy of amide I is stronger than that of amide II, which is due to greater bond strength (double bond) of amide I whereas amide II absorption is caused by lesser bond strength (single bond). Second, the anisotropy of amide I absorption is opposite to that of amide II for all three sections, that ensures the perpendicularity of transition moment directions of these amide bonds. For the parallel and regular sections of tendon, since the fibril's long axis is parallel to the x-axis of the moving stage in both orientations, their infrared anisotropy is similar to that of the radial zone fibrils in regular sections of articular cartilage (the amide I anisotropy has a maximum at 0° and a minimum at 90°; and the same for amide II is opposite [23,24]). An interesting result is the amide anisotropy in the cross sections – though the anisotropy is weaker compared to the same in other two surfaces, the angular dependency remains the same.


Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

Absorption anisotropy of amide I (a) and amide II (b) of tendon in the regular, parallel and cross sections.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570663&req=5

Figure 3: Absorption anisotropy of amide I (a) and amide II (b) of tendon in the regular, parallel and cross sections.
Mentions: Figure 3 depicts the absorption anisotropy of amide I and amide II in tendon's regular, parallel and cross sections. Two features can be observed. First, the absorption anisotropy of amide I is stronger than that of amide II, which is due to greater bond strength (double bond) of amide I whereas amide II absorption is caused by lesser bond strength (single bond). Second, the anisotropy of amide I absorption is opposite to that of amide II for all three sections, that ensures the perpendicularity of transition moment directions of these amide bonds. For the parallel and regular sections of tendon, since the fibril's long axis is parallel to the x-axis of the moving stage in both orientations, their infrared anisotropy is similar to that of the radial zone fibrils in regular sections of articular cartilage (the amide I anisotropy has a maximum at 0° and a minimum at 90°; and the same for amide II is opposite [23,24]). An interesting result is the amide anisotropy in the cross sections – though the anisotropy is weaker compared to the same in other two surfaces, the angular dependency remains the same.

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


Related in: MedlinePlus