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Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

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Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


Related in: MedlinePlus

The visible images from the FTIR imager, tendon (a) and cartilage (b). (a.s. – articular surface).
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Figure 2: The visible images from the FTIR imager, tendon (a) and cartilage (b). (a.s. – articular surface).

Mentions: Figure 2 shows the visible images of tendon and cartilage sections from different surfaces of the tissue block. It is evident that the regular and parallel sections of tendon have similar fibril morphology, with the tendon fibrils running parallel in the plane of the tissue section. In contrast, the cross section of tendon has very different morphology. For articular cartilage, the regular section contains three typical histological zones; whereas each parallel section of cartilage has a very different fibril orientation, depending upon the depth at which the section is obtained.


Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage.

Ramakrishnan N, Xia Y, Bidthanapally A - J Orthop Surg Res (2008)

The visible images from the FTIR imager, tendon (a) and cartilage (b). (a.s. – articular surface).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570663&req=5

Figure 2: The visible images from the FTIR imager, tendon (a) and cartilage (b). (a.s. – articular surface).
Mentions: Figure 2 shows the visible images of tendon and cartilage sections from different surfaces of the tissue block. It is evident that the regular and parallel sections of tendon have similar fibril morphology, with the tendon fibrils running parallel in the plane of the tissue section. In contrast, the cross section of tendon has very different morphology. For articular cartilage, the regular section contains three typical histological zones; whereas each parallel section of cartilage has a very different fibril orientation, depending upon the depth at which the section is obtained.

Bottom Line: With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.

ABSTRACT

Background: Fourier Transform Infrared Imaging (FTIRI) is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light, the resulting anisotropic behavior of the tissue structure is described here.

Methods: Thin sections (6 mum thick) were obtained from three different surfaces of the canine tissue blocks and imaged at 6.25 microm pixel resolution. For each section, infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 degrees increment of the analyzer's angle in the 0 degrees-180 degrees angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces.

Results: For tendon, the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections; and tendon's cross sections show distinct, but weak anisotropic behavior for both the amide components. For articular cartilage, parallel sections in the superficial zone have the expected infrared anisotropy that is consistent with that of regular sections. The parallel sections in the radial zone, however, have a nearly isotropic amide II absorption and a distinct amide I anisotropy.

Conclusion: From the inconsistency in amide anisotropy between superficial to radial zone in parallel section results, a schematic model is used to explain the origins of these amide anisotropies in cartilage and tendon.

No MeSH data available.


Related in: MedlinePlus