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Expression of endoplasmic reticulum chaperones in cardiac development.

Papp S, Zhang X, Szabo E, Michalak M, Opas M - Open Cardiovasc Med J (2008)

Bottom Line: We found stress related chaperones were more abundant in embryonic compared to adult hearts, indicating endoplasmic reticulum stress during normal cardiac development.In adult hearts, chaperones are less abundant but there are increased levels of ATF6alpha and ER stress-activated caspases.Thus, protein synthesis during embryonic development does not seem to be as intense a stress as is required for apoptosis that is found during postnatal remodelling.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
To determine if cardiogenesis causes endoplasmic reticulum stress, we examined chaperone expression. Many cardiac pathologies cause activation of the fetal gene program, and we asked the reverse: could activation of the fetal gene program during development induce endoplasmic reticulum stress/chaperones? We found stress related chaperones were more abundant in embryonic compared to adult hearts, indicating endoplasmic reticulum stress during normal cardiac development. To determine the degree of stress, we investigated endoplasmic reticulum stress pathways during cardiogenesis. We detected higher levels of ATF6alpha, caspase 7 and 12 in adult hearts. Thus, during embryonic development, there is large protein synthetic load but there is no endoplasmic reticulum stress. In adult hearts, chaperones are less abundant but there are increased levels of ATF6alpha and ER stress-activated caspases. Thus, protein synthesis during embryonic development does not seem to be as intense a stress as is required for apoptosis that is found during postnatal remodelling.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of caspase-7 and caspase-12 during heart development.  .Both caspases are more abundant in embryonic ventricles compared to embryonic atria. Importantly, these caspases are more abundant in adult heart tissues, especially the ventricles, compared to em-bryonic heart tissues. GAPDH was used as a loading control.E – embryonic day; H – heart; At – atria; V – ventricles
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Figure 6: Western blot analysis of caspase-7 and caspase-12 during heart development. .Both caspases are more abundant in embryonic ventricles compared to embryonic atria. Importantly, these caspases are more abundant in adult heart tissues, especially the ventricles, compared to em-bryonic heart tissues. GAPDH was used as a loading control.E – embryonic day; H – heart; At – atria; V – ventricles

Mentions: To further determine the degree of ER stress in developing and adult mouse hearts, Western blot analysis was performed for caspase-7 and caspase-12, which have been shown to be activated during ER stress [33, 34]. The antibodies used here were able to detect both pro-caspases and cleaved caspases 7 and 12. Fig. (6) shows that both pro-caspases and cleaved caspases were much more abundant in embryonic ventricles than in atria. More importantly, these caspases were more abundant in adult hearts compared to embryonic hearts, suggesting a greater degree of apoptosis in adult hearts, which may be related to cardiac remodelling.


Expression of endoplasmic reticulum chaperones in cardiac development.

Papp S, Zhang X, Szabo E, Michalak M, Opas M - Open Cardiovasc Med J (2008)

Western blot analysis of caspase-7 and caspase-12 during heart development.  .Both caspases are more abundant in embryonic ventricles compared to embryonic atria. Importantly, these caspases are more abundant in adult heart tissues, especially the ventricles, compared to em-bryonic heart tissues. GAPDH was used as a loading control.E – embryonic day; H – heart; At – atria; V – ventricles
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570582&req=5

Figure 6: Western blot analysis of caspase-7 and caspase-12 during heart development. .Both caspases are more abundant in embryonic ventricles compared to embryonic atria. Importantly, these caspases are more abundant in adult heart tissues, especially the ventricles, compared to em-bryonic heart tissues. GAPDH was used as a loading control.E – embryonic day; H – heart; At – atria; V – ventricles
Mentions: To further determine the degree of ER stress in developing and adult mouse hearts, Western blot analysis was performed for caspase-7 and caspase-12, which have been shown to be activated during ER stress [33, 34]. The antibodies used here were able to detect both pro-caspases and cleaved caspases 7 and 12. Fig. (6) shows that both pro-caspases and cleaved caspases were much more abundant in embryonic ventricles than in atria. More importantly, these caspases were more abundant in adult hearts compared to embryonic hearts, suggesting a greater degree of apoptosis in adult hearts, which may be related to cardiac remodelling.

Bottom Line: We found stress related chaperones were more abundant in embryonic compared to adult hearts, indicating endoplasmic reticulum stress during normal cardiac development.In adult hearts, chaperones are less abundant but there are increased levels of ATF6alpha and ER stress-activated caspases.Thus, protein synthesis during embryonic development does not seem to be as intense a stress as is required for apoptosis that is found during postnatal remodelling.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
To determine if cardiogenesis causes endoplasmic reticulum stress, we examined chaperone expression. Many cardiac pathologies cause activation of the fetal gene program, and we asked the reverse: could activation of the fetal gene program during development induce endoplasmic reticulum stress/chaperones? We found stress related chaperones were more abundant in embryonic compared to adult hearts, indicating endoplasmic reticulum stress during normal cardiac development. To determine the degree of stress, we investigated endoplasmic reticulum stress pathways during cardiogenesis. We detected higher levels of ATF6alpha, caspase 7 and 12 in adult hearts. Thus, during embryonic development, there is large protein synthetic load but there is no endoplasmic reticulum stress. In adult hearts, chaperones are less abundant but there are increased levels of ATF6alpha and ER stress-activated caspases. Thus, protein synthesis during embryonic development does not seem to be as intense a stress as is required for apoptosis that is found during postnatal remodelling.

No MeSH data available.


Related in: MedlinePlus