Limits...
Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats.

Dallak MM, Mikhailidis DP, Haidara MA, Bin-Jaliah IM, Tork OM, Rateb MA, Yassin HZ, Al-Refaie ZA, Ibrahim IM, Elawa SM, Rashed LA, Afifi NA - Open Cardiovasc Med J (2008)

Bottom Line: Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group.Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals.Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

View Article: PubMed Central - PubMed

Affiliation: Physiology Department, College of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Aim: To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.

Materials and methods: This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage

Results: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone

Conclusion: Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals. Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

No MeSH data available.


Related in: MedlinePlus

Electron micrograph of an ultrathin section in the ventricular muscle of group II (diabetic rats) showing that the nucleus of a cardiac myocyte (N) has irregular nuclear outline (arrows) with margination of its chromatin. Cardiac myocytes are rarefied with abundant vacuoles (V) and dispersed mitochondria with dense ma-trix (m) (Original mag. X 3000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2570581&req=5

Figure 7: Electron micrograph of an ultrathin section in the ventricular muscle of group II (diabetic rats) showing that the nucleus of a cardiac myocyte (N) has irregular nuclear outline (arrows) with margination of its chromatin. Cardiac myocytes are rarefied with abundant vacuoles (V) and dispersed mitochondria with dense ma-trix (m) (Original mag. X 3000).

Mentions: Electron microscopic examination of ultra-thin sections of the left ventricular myocardium of the control group (group I) revealed normal histological structure of cardiac myocytes Fig. (3). Ventricular sections obtained from untreated rats (group II) showed myofibrillar lysis in the form marked degeneration, disruption and rarefaction of the myofibrils (Figs. 4, 5). Nuclei of cardiac myocytes exhibited several degenerative changes where some nuclei showed peripherally condensed marginated chromatin (Fig. 6). In addition several cardiac myocytes exhibited marked cytoplasmic vacuolations (Fig. 7).


Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats.

Dallak MM, Mikhailidis DP, Haidara MA, Bin-Jaliah IM, Tork OM, Rateb MA, Yassin HZ, Al-Refaie ZA, Ibrahim IM, Elawa SM, Rashed LA, Afifi NA - Open Cardiovasc Med J (2008)

Electron micrograph of an ultrathin section in the ventricular muscle of group II (diabetic rats) showing that the nucleus of a cardiac myocyte (N) has irregular nuclear outline (arrows) with margination of its chromatin. Cardiac myocytes are rarefied with abundant vacuoles (V) and dispersed mitochondria with dense ma-trix (m) (Original mag. X 3000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570581&req=5

Figure 7: Electron micrograph of an ultrathin section in the ventricular muscle of group II (diabetic rats) showing that the nucleus of a cardiac myocyte (N) has irregular nuclear outline (arrows) with margination of its chromatin. Cardiac myocytes are rarefied with abundant vacuoles (V) and dispersed mitochondria with dense ma-trix (m) (Original mag. X 3000).
Mentions: Electron microscopic examination of ultra-thin sections of the left ventricular myocardium of the control group (group I) revealed normal histological structure of cardiac myocytes Fig. (3). Ventricular sections obtained from untreated rats (group II) showed myofibrillar lysis in the form marked degeneration, disruption and rarefaction of the myofibrils (Figs. 4, 5). Nuclei of cardiac myocytes exhibited several degenerative changes where some nuclei showed peripherally condensed marginated chromatin (Fig. 6). In addition several cardiac myocytes exhibited marked cytoplasmic vacuolations (Fig. 7).

Bottom Line: Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group.Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals.Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

View Article: PubMed Central - PubMed

Affiliation: Physiology Department, College of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Aim: To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.

Materials and methods: This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage

Results: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone

Conclusion: Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals. Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

No MeSH data available.


Related in: MedlinePlus