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Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats.

Dallak MM, Mikhailidis DP, Haidara MA, Bin-Jaliah IM, Tork OM, Rateb MA, Yassin HZ, Al-Refaie ZA, Ibrahim IM, Elawa SM, Rashed LA, Afifi NA - Open Cardiovasc Med J (2008)

Bottom Line: Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group.Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals.Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

View Article: PubMed Central - PubMed

Affiliation: Physiology Department, College of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Aim: To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.

Materials and methods: This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage

Results: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone

Conclusion: Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals. Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

No MeSH data available.


Related in: MedlinePlus

An agarose gel electrophoresis showing product of iNOS gene expression. Lane M: PCR marker. Lane 1: gene product in control group. Lane 2, 3&4: gene product in diabetic group. Lane 5: gene product in diabetic group receive insulin. Lane 6: gene product in diabetic group receive vitamin E. Lane 7: gene product in diabetic group receive insulin and vit E
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Figure 2: An agarose gel electrophoresis showing product of iNOS gene expression. Lane M: PCR marker. Lane 1: gene product in control group. Lane 2, 3&4: gene product in diabetic group. Lane 5: gene product in diabetic group receive insulin. Lane 6: gene product in diabetic group receive vitamin E. Lane 7: gene product in diabetic group receive insulin and vit E

Mentions: The amplified PCR product of selected gene were electrophoresed on 1.5 % Agarose gel and were UV visualized after staining with ethidium bromide. UV illuminated gel were photographed. A densitometry system using a Standard DNA of known concentration gene Gel, documentation system was used for analysis (Syngene, Cambridge, UK), Figs. (1 & 2).


Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats.

Dallak MM, Mikhailidis DP, Haidara MA, Bin-Jaliah IM, Tork OM, Rateb MA, Yassin HZ, Al-Refaie ZA, Ibrahim IM, Elawa SM, Rashed LA, Afifi NA - Open Cardiovasc Med J (2008)

An agarose gel electrophoresis showing product of iNOS gene expression. Lane M: PCR marker. Lane 1: gene product in control group. Lane 2, 3&4: gene product in diabetic group. Lane 5: gene product in diabetic group receive insulin. Lane 6: gene product in diabetic group receive vitamin E. Lane 7: gene product in diabetic group receive insulin and vit E
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570581&req=5

Figure 2: An agarose gel electrophoresis showing product of iNOS gene expression. Lane M: PCR marker. Lane 1: gene product in control group. Lane 2, 3&4: gene product in diabetic group. Lane 5: gene product in diabetic group receive insulin. Lane 6: gene product in diabetic group receive vitamin E. Lane 7: gene product in diabetic group receive insulin and vit E
Mentions: The amplified PCR product of selected gene were electrophoresed on 1.5 % Agarose gel and were UV visualized after staining with ethidium bromide. UV illuminated gel were photographed. A densitometry system using a Standard DNA of known concentration gene Gel, documentation system was used for analysis (Syngene, Cambridge, UK), Figs. (1 & 2).

Bottom Line: Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group.Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals.Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

View Article: PubMed Central - PubMed

Affiliation: Physiology Department, College of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Aim: To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.

Materials and methods: This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage

Results: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone

Conclusion: Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals. Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications.

No MeSH data available.


Related in: MedlinePlus