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In vivo and in vitro assessment of human saphenous vein wall changes.

Asbeutah AM, Asfar SK, Safar H, Oriowo MA, Elhagrassi I, Abu-Assi MA, Cameron JD, McGrath BP - Open Cardiovasc Med J (2007)

Bottom Line: A total of 10 normal healthy volunteers were selected for comparison.The repeated measure ANOVA test for the diameter, percent, and difference changes of GSV diameter from maximum diameter at different time intervals showed significance difference within and between all groups.The venous endothelial damage may cause vascular smooth muscle contractions dysfunction that favours dilatation and secondary valvular insufficiency.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Monash University and Department of Vascular Sciences in Southern Health, Dandenong Hospital, Melbourne, Australia.

ABSTRACT

Purpose: To investigate if noradrenaline (NA) and 5-hydroxyptamine (5-HT) drugs induce responses of isolated control and varicose veins are altered by removal of the endothelium. SUBJECTS #ENTITYSTARTX00026;

Methods: Specimens of the great saphenous vein (GSV) were obtained from 12 subjects with primary varicose veins and 12 subjects from donor vessels at cardiac surgery. A total of 10 normal healthy volunteers were selected for comparison. The diameter changes of GSV during the resting phase, at the end of 5 minutes occlusion, and then every 30 seconds post deflation for five minutes were measured using B-mode ultrasound. Post-surgery the vein sample was collected in a tube of Krebs-Henseleit solution.

Results: The repeated measure ANOVA test for the diameter, percent, and difference changes of GSV diameter from maximum diameter at different time intervals showed significance difference within and between all groups. NA and 5-HT produced concentration-dependent contractions of control and varicose saphenous vein segments. There was no significant difference in the potency of NA and for 5-HT, but the maximum response, normalized for tissue weight, was less in varicose vein segments. Removal of the endothelium had no effect on the potency of NA or 5-HT but significantly (p<0.05) reduced the maximum response to NA and 5-HT in varicose vein segments but not to 5-HT in control veins.

Conclusion: The venous endothelial damage may cause vascular smooth muscle contractions dysfunction that favours dilatation and secondary valvular insufficiency.

No MeSH data available.


Related in: MedlinePlus

Bar graphs of human great saphenous vein rings to increasing concentration of (a) NA and (b) 5-HT for control and varicose vein segments. Endothelium (+) and rubbed (-) in each control (Cv) and varicose veins (Vv) specimens.                        *p<0.05 Cv+ compared to Vv+ and # p<0.05 Vv+ compared to Vv- using Student’s paired t-test.
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Figure 2: Bar graphs of human great saphenous vein rings to increasing concentration of (a) NA and (b) 5-HT for control and varicose vein segments. Endothelium (+) and rubbed (-) in each control (Cv) and varicose veins (Vv) specimens. *p<0.05 Cv+ compared to Vv+ and # p<0.05 Vv+ compared to Vv- using Student’s paired t-test.

Mentions: NA and 5-HT produced concentration-dependent contractions of control and varicose saphenous vein segments. There was no significant difference in the potency of NA (log EC50 values were -5.55±0.06 and -5.58±0.10, n =6, in control and varicose vein segments, respectively) and for 5-HT (log EC50 values were -6.44±0.08 and -6.66±0.09, n=5, in control and varicose vein segments, respectively) between the two groups but the maximum response, normalized for tissue weight, was less in varicose vein segments for both NA and 5-HT. Results are represented in Fig. (2a,b) and Table 4.


In vivo and in vitro assessment of human saphenous vein wall changes.

Asbeutah AM, Asfar SK, Safar H, Oriowo MA, Elhagrassi I, Abu-Assi MA, Cameron JD, McGrath BP - Open Cardiovasc Med J (2007)

Bar graphs of human great saphenous vein rings to increasing concentration of (a) NA and (b) 5-HT for control and varicose vein segments. Endothelium (+) and rubbed (-) in each control (Cv) and varicose veins (Vv) specimens.                        *p<0.05 Cv+ compared to Vv+ and # p<0.05 Vv+ compared to Vv- using Student’s paired t-test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2570569&req=5

Figure 2: Bar graphs of human great saphenous vein rings to increasing concentration of (a) NA and (b) 5-HT for control and varicose vein segments. Endothelium (+) and rubbed (-) in each control (Cv) and varicose veins (Vv) specimens. *p<0.05 Cv+ compared to Vv+ and # p<0.05 Vv+ compared to Vv- using Student’s paired t-test.
Mentions: NA and 5-HT produced concentration-dependent contractions of control and varicose saphenous vein segments. There was no significant difference in the potency of NA (log EC50 values were -5.55±0.06 and -5.58±0.10, n =6, in control and varicose vein segments, respectively) and for 5-HT (log EC50 values were -6.44±0.08 and -6.66±0.09, n=5, in control and varicose vein segments, respectively) between the two groups but the maximum response, normalized for tissue weight, was less in varicose vein segments for both NA and 5-HT. Results are represented in Fig. (2a,b) and Table 4.

Bottom Line: A total of 10 normal healthy volunteers were selected for comparison.The repeated measure ANOVA test for the diameter, percent, and difference changes of GSV diameter from maximum diameter at different time intervals showed significance difference within and between all groups.The venous endothelial damage may cause vascular smooth muscle contractions dysfunction that favours dilatation and secondary valvular insufficiency.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Monash University and Department of Vascular Sciences in Southern Health, Dandenong Hospital, Melbourne, Australia.

ABSTRACT

Purpose: To investigate if noradrenaline (NA) and 5-hydroxyptamine (5-HT) drugs induce responses of isolated control and varicose veins are altered by removal of the endothelium. SUBJECTS #ENTITYSTARTX00026;

Methods: Specimens of the great saphenous vein (GSV) were obtained from 12 subjects with primary varicose veins and 12 subjects from donor vessels at cardiac surgery. A total of 10 normal healthy volunteers were selected for comparison. The diameter changes of GSV during the resting phase, at the end of 5 minutes occlusion, and then every 30 seconds post deflation for five minutes were measured using B-mode ultrasound. Post-surgery the vein sample was collected in a tube of Krebs-Henseleit solution.

Results: The repeated measure ANOVA test for the diameter, percent, and difference changes of GSV diameter from maximum diameter at different time intervals showed significance difference within and between all groups. NA and 5-HT produced concentration-dependent contractions of control and varicose saphenous vein segments. There was no significant difference in the potency of NA and for 5-HT, but the maximum response, normalized for tissue weight, was less in varicose vein segments. Removal of the endothelium had no effect on the potency of NA or 5-HT but significantly (p<0.05) reduced the maximum response to NA and 5-HT in varicose vein segments but not to 5-HT in control veins.

Conclusion: The venous endothelial damage may cause vascular smooth muscle contractions dysfunction that favours dilatation and secondary valvular insufficiency.

No MeSH data available.


Related in: MedlinePlus