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The immature heart: the roles of bone marrow stromal stem cells in growth and myocardial repair.

Jun L, Minh D, Calvin W, Carolyn J T, Ray C J C, Dominique ST - Open Cardiovasc Med J (2007)

Bottom Line: Labeled MSCs were found to home into the bone marrow in all rats of different developmental stages.In contrast to that reported in the developing fetus, MSCs did not appear to contribute to the growth of non-injured hearts after birth.However, they can be recruited from the bone marrow and regenerate damaged myocardium both in the adult and in the immature hearts.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiothoracic Surgery, the Montreal General Hospital, MUHC, Canada.

ABSTRACT
Studies have shown that adult bone marrow derived stem cells (MSCs) can participate in repair of myocardial injury in adult hearts, as well as in cardiac growth during fetal development in utero. Yet, no studies have evaluated the role of MSCs with respect to normal growth or tissue repair in immature hearts after birth. The present study examines whether MSCs may participate in the myocardial growth and injury in the post-natal immature hearts. MSCs were isolated from adult Lewis rats and labeled with Lac-Z gene using retroviral vectors. These MSCs were injected systemically into groups of neonatal (NB=2days-old), immature (B=30days-old) and adult (A=>3months-old) isogeneic Lewis rats. Additionally, left coronary artery ligation was carried out in subgroups of immature (BL) and adult (AL) rats one week after MSCs injection. The hearts were harvested serially from 2-days to 6-weeks, stained with X-Gal for labeled MSCs. Cardiomyocyte phenotypic expression was evaluated by immunohistological staining for Troponin I-C and Connexin-43. Labeled MSCs were found to home into the bone marrow in all rats of different developmental stages. They could be recruited from bone marrow into the infarcted site of myocardium only in groups AL and BL. They were also capable of differentiating into cardiomyocyte phenotype after myocardial injury. In contrast to that reported in the developing fetus, MSCs did not appear to contribute to the growth of non-injured hearts after birth. However, they can be recruited from the bone marrow and regenerate damaged myocardium both in the adult and in the immature hearts.

No MeSH data available.


Related in: MedlinePlus

Body weight changes in groups A, B and NB. Note body weights in groups B, NB have significantly increased over 3 weeks period. * indicates p<0.05 compared with corresponding weight at day 0.
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Figure 2: Body weight changes in groups A, B and NB. Note body weights in groups B, NB have significantly increased over 3 weeks period. * indicates p<0.05 compared with corresponding weight at day 0.

Mentions: The body weight in group B and group NB had significantly grown from 63.1±6.7g to 207.8±7.6g and from 7.4±1.1g to 50.2±4.0g (p<0.05), respectively (Fig. 2), at 6 weeks after MSC implantation (Table 2). Yet, we were not able to detect any labeled cells in the heart samples taken from groups A, B, and NB (Fig.1B) at any time points ranging from 2 days to 6 weeks after implantations when stained with X-gal solution.


The immature heart: the roles of bone marrow stromal stem cells in growth and myocardial repair.

Jun L, Minh D, Calvin W, Carolyn J T, Ray C J C, Dominique ST - Open Cardiovasc Med J (2007)

Body weight changes in groups A, B and NB. Note body weights in groups B, NB have significantly increased over 3 weeks period. * indicates p<0.05 compared with corresponding weight at day 0.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570564&req=5

Figure 2: Body weight changes in groups A, B and NB. Note body weights in groups B, NB have significantly increased over 3 weeks period. * indicates p<0.05 compared with corresponding weight at day 0.
Mentions: The body weight in group B and group NB had significantly grown from 63.1±6.7g to 207.8±7.6g and from 7.4±1.1g to 50.2±4.0g (p<0.05), respectively (Fig. 2), at 6 weeks after MSC implantation (Table 2). Yet, we were not able to detect any labeled cells in the heart samples taken from groups A, B, and NB (Fig.1B) at any time points ranging from 2 days to 6 weeks after implantations when stained with X-gal solution.

Bottom Line: Labeled MSCs were found to home into the bone marrow in all rats of different developmental stages.In contrast to that reported in the developing fetus, MSCs did not appear to contribute to the growth of non-injured hearts after birth.However, they can be recruited from the bone marrow and regenerate damaged myocardium both in the adult and in the immature hearts.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiothoracic Surgery, the Montreal General Hospital, MUHC, Canada.

ABSTRACT
Studies have shown that adult bone marrow derived stem cells (MSCs) can participate in repair of myocardial injury in adult hearts, as well as in cardiac growth during fetal development in utero. Yet, no studies have evaluated the role of MSCs with respect to normal growth or tissue repair in immature hearts after birth. The present study examines whether MSCs may participate in the myocardial growth and injury in the post-natal immature hearts. MSCs were isolated from adult Lewis rats and labeled with Lac-Z gene using retroviral vectors. These MSCs were injected systemically into groups of neonatal (NB=2days-old), immature (B=30days-old) and adult (A=>3months-old) isogeneic Lewis rats. Additionally, left coronary artery ligation was carried out in subgroups of immature (BL) and adult (AL) rats one week after MSCs injection. The hearts were harvested serially from 2-days to 6-weeks, stained with X-Gal for labeled MSCs. Cardiomyocyte phenotypic expression was evaluated by immunohistological staining for Troponin I-C and Connexin-43. Labeled MSCs were found to home into the bone marrow in all rats of different developmental stages. They could be recruited from bone marrow into the infarcted site of myocardium only in groups AL and BL. They were also capable of differentiating into cardiomyocyte phenotype after myocardial injury. In contrast to that reported in the developing fetus, MSCs did not appear to contribute to the growth of non-injured hearts after birth. However, they can be recruited from the bone marrow and regenerate damaged myocardium both in the adult and in the immature hearts.

No MeSH data available.


Related in: MedlinePlus