Limits...
Subunit Mobility and the Chaperone Activity of Recombinant alphaB-Crystallin.

Krushelnitsky A, Mukhametshina N, Gogolev Y, Tarasova N, Faizullin D, Zinkevich T, Gnezdilov O, Fedotov V - Open Biochem J (2008)

Bottom Line: The comparison of the chaperone-like activity of native and covalently cross-linked human alphaB-crystallins has confirmed the important role of the subunit mobility in the chaperoning mechanism.Our data clearly demonstrate that the chaperone-like activity of alpha-crystallin is not only a surface phenomenon as was suggested by some researchers.

View Article: PubMed Central - PubMed

Affiliation: Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia.

ABSTRACT
The comparison of the chaperone-like activity of native and covalently cross-linked human alphaB-crystallins has confirmed the important role of the subunit mobility in the chaperoning mechanism. Our data clearly demonstrate that the chaperone-like activity of alpha-crystallin is not only a surface phenomenon as was suggested by some researchers.

No MeSH data available.


Related in: MedlinePlus

Diffusion decays for the native and cross-linked αB-crystallins. Solid lines are the fitting curves plotted according to Eq. (1). The SDC are 2.6×10-11 and 2.4×10-11 m2/s for the native and cross-linked αB-crystallins, respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2570560&req=5

Figure 3: Diffusion decays for the native and cross-linked αB-crystallins. Solid lines are the fitting curves plotted according to Eq. (1). The SDC are 2.6×10-11 and 2.4×10-11 m2/s for the native and cross-linked αB-crystallins, respectively

Mentions: Fig. (3) presents the diffusion decays for the native and cross-linked αB-crystallins. The SDC values correspond quite well to the previous light scattering self-diffusion measurements of α-crystallin [22]. Note that the diffusion decays could be well approximated by a single SDC. It is known that SDC is proportional to the linear size of a Brownian particle, hence, SDC~M0.33, where M is a molecular weight. Thus, the shape of the diffusion decays is not very sensitive to molecular mass distributions. However, our data indicate that the molecular mass distribution of the αB-crystallin molecules is at least not very wide. So, two independent checks performed by Augusteyn [17] and by us confirm that glutaraldehyde cross-linking does not change the size of α-crystallin oligomers.


Subunit Mobility and the Chaperone Activity of Recombinant alphaB-Crystallin.

Krushelnitsky A, Mukhametshina N, Gogolev Y, Tarasova N, Faizullin D, Zinkevich T, Gnezdilov O, Fedotov V - Open Biochem J (2008)

Diffusion decays for the native and cross-linked αB-crystallins. Solid lines are the fitting curves plotted according to Eq. (1). The SDC are 2.6×10-11 and 2.4×10-11 m2/s for the native and cross-linked αB-crystallins, respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2570560&req=5

Figure 3: Diffusion decays for the native and cross-linked αB-crystallins. Solid lines are the fitting curves plotted according to Eq. (1). The SDC are 2.6×10-11 and 2.4×10-11 m2/s for the native and cross-linked αB-crystallins, respectively
Mentions: Fig. (3) presents the diffusion decays for the native and cross-linked αB-crystallins. The SDC values correspond quite well to the previous light scattering self-diffusion measurements of α-crystallin [22]. Note that the diffusion decays could be well approximated by a single SDC. It is known that SDC is proportional to the linear size of a Brownian particle, hence, SDC~M0.33, where M is a molecular weight. Thus, the shape of the diffusion decays is not very sensitive to molecular mass distributions. However, our data indicate that the molecular mass distribution of the αB-crystallin molecules is at least not very wide. So, two independent checks performed by Augusteyn [17] and by us confirm that glutaraldehyde cross-linking does not change the size of α-crystallin oligomers.

Bottom Line: The comparison of the chaperone-like activity of native and covalently cross-linked human alphaB-crystallins has confirmed the important role of the subunit mobility in the chaperoning mechanism.Our data clearly demonstrate that the chaperone-like activity of alpha-crystallin is not only a surface phenomenon as was suggested by some researchers.

View Article: PubMed Central - PubMed

Affiliation: Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia.

ABSTRACT
The comparison of the chaperone-like activity of native and covalently cross-linked human alphaB-crystallins has confirmed the important role of the subunit mobility in the chaperoning mechanism. Our data clearly demonstrate that the chaperone-like activity of alpha-crystallin is not only a surface phenomenon as was suggested by some researchers.

No MeSH data available.


Related in: MedlinePlus