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Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion.

Okumura Y, Yamamoto Y, Zhang Z, Toyama T, Kawasoe T, Ibusuki M, Honda Y, Iyama K, Yamashita H, Iwase H - BMC Cancer (2008)

Bottom Line: The frequency of necrosis and positive expression ratio of survivin and Bax were significantly higher in DCIS-Mi than in DCIS.Multivariate analysis revealed that the presence of necrosis and positive survivin expression were independent factors associated with invasion.Furthermore, the differential expression of survivin may serve in deciding the response to therapy and may have some prognostic significance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast and Endocrine Surgery, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-8556, Japan. sigeoku@yahoo.co.jp

ABSTRACT

Background: Widespread use of mammography in breast cancer screening has led to the identification of increasing numbers of patients with ductal carcinoma in situ (DCIS). DCIS of the breast with an area of focal invasion 1 mm or less in diameter is defined as DCIS with microinvasion, DCIS-Mi. Identification of biological differences between DCIS and DCIS-Mi may aid in understanding of the nature and causes of the progression of DCIS to invasiveness.

Methods: In this study, using resected breast cancer tissues, we compared pure DCIS (52 cases) and DCIS-Mi (28 cases) with regard to pathological findings of intraductal lesions, biological factors, apoptosis-related protein expression, and proliferative capacity through the use of immunohistochemistry and the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method.

Results: There were no differences in biological factors between DCIS and DCIS-Mi, with respect to levels of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor type 2. The frequency of necrosis and positive expression ratio of survivin and Bax were significantly higher in DCIS-Mi than in DCIS. In addition, apoptotic index, Ki-67 index, and positive Bcl-2 immunolabeling tended to be higher in DCIS-Mi than in DCIS. Multivariate analysis revealed that the presence of necrosis and positive survivin expression were independent factors associated with invasion.

Conclusion: Compared with DCIS, DCIS-Mi is characterized by a slightly elevated cell proliferation capacity and enhanced apoptosis within the intraductal lesion, both of which are thought to promote the formation of cell necrotic foci. Furthermore, the differential expression of survivin may serve in deciding the response to therapy and may have some prognostic significance.

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Representative microscopic views of immunohistochemical detection. Intraductal component found in patients with DCIS, using A. hematoxylin-eosin (HE) staining, the frames show cells stained for B. estrogen receptor (ER), C. HER2, D. Ki-67, E. p21, F. BCL-2, G. BAX, and H. Survivin 1, respectively.  I. Survivin 2 shows moderately positive Survivin staining detected in another patient with DCIS. (original magnification × 200)
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Figure 1: Representative microscopic views of immunohistochemical detection. Intraductal component found in patients with DCIS, using A. hematoxylin-eosin (HE) staining, the frames show cells stained for B. estrogen receptor (ER), C. HER2, D. Ki-67, E. p21, F. BCL-2, G. BAX, and H. Survivin 1, respectively. I. Survivin 2 shows moderately positive Survivin staining detected in another patient with DCIS. (original magnification × 200)

Mentions: There was no significant difference between DCIS and DCIS-Mi in expression levels of universal biological factors such as ER, PgR, and HER2. The Ki-67 index tended to be higher in DCIS-Mi (22.8 ± 2.0%) than in DCIS (17.9 ± 1.5%), although the difference was not statistically significant (P = 0.052) (Table 3). Representative microscopic views of immunohistochemical detection were shown in Fig. 1.


Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion.

Okumura Y, Yamamoto Y, Zhang Z, Toyama T, Kawasoe T, Ibusuki M, Honda Y, Iyama K, Yamashita H, Iwase H - BMC Cancer (2008)

Representative microscopic views of immunohistochemical detection. Intraductal component found in patients with DCIS, using A. hematoxylin-eosin (HE) staining, the frames show cells stained for B. estrogen receptor (ER), C. HER2, D. Ki-67, E. p21, F. BCL-2, G. BAX, and H. Survivin 1, respectively.  I. Survivin 2 shows moderately positive Survivin staining detected in another patient with DCIS. (original magnification × 200)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2567990&req=5

Figure 1: Representative microscopic views of immunohistochemical detection. Intraductal component found in patients with DCIS, using A. hematoxylin-eosin (HE) staining, the frames show cells stained for B. estrogen receptor (ER), C. HER2, D. Ki-67, E. p21, F. BCL-2, G. BAX, and H. Survivin 1, respectively. I. Survivin 2 shows moderately positive Survivin staining detected in another patient with DCIS. (original magnification × 200)
Mentions: There was no significant difference between DCIS and DCIS-Mi in expression levels of universal biological factors such as ER, PgR, and HER2. The Ki-67 index tended to be higher in DCIS-Mi (22.8 ± 2.0%) than in DCIS (17.9 ± 1.5%), although the difference was not statistically significant (P = 0.052) (Table 3). Representative microscopic views of immunohistochemical detection were shown in Fig. 1.

Bottom Line: The frequency of necrosis and positive expression ratio of survivin and Bax were significantly higher in DCIS-Mi than in DCIS.Multivariate analysis revealed that the presence of necrosis and positive survivin expression were independent factors associated with invasion.Furthermore, the differential expression of survivin may serve in deciding the response to therapy and may have some prognostic significance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast and Endocrine Surgery, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-8556, Japan. sigeoku@yahoo.co.jp

ABSTRACT

Background: Widespread use of mammography in breast cancer screening has led to the identification of increasing numbers of patients with ductal carcinoma in situ (DCIS). DCIS of the breast with an area of focal invasion 1 mm or less in diameter is defined as DCIS with microinvasion, DCIS-Mi. Identification of biological differences between DCIS and DCIS-Mi may aid in understanding of the nature and causes of the progression of DCIS to invasiveness.

Methods: In this study, using resected breast cancer tissues, we compared pure DCIS (52 cases) and DCIS-Mi (28 cases) with regard to pathological findings of intraductal lesions, biological factors, apoptosis-related protein expression, and proliferative capacity through the use of immunohistochemistry and the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method.

Results: There were no differences in biological factors between DCIS and DCIS-Mi, with respect to levels of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor type 2. The frequency of necrosis and positive expression ratio of survivin and Bax were significantly higher in DCIS-Mi than in DCIS. In addition, apoptotic index, Ki-67 index, and positive Bcl-2 immunolabeling tended to be higher in DCIS-Mi than in DCIS. Multivariate analysis revealed that the presence of necrosis and positive survivin expression were independent factors associated with invasion.

Conclusion: Compared with DCIS, DCIS-Mi is characterized by a slightly elevated cell proliferation capacity and enhanced apoptosis within the intraductal lesion, both of which are thought to promote the formation of cell necrotic foci. Furthermore, the differential expression of survivin may serve in deciding the response to therapy and may have some prognostic significance.

Show MeSH
Related in: MedlinePlus