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Exercise training in the aerobic/anaerobic metabolic transition prevents glucose intolerance in alloxan-treated rats.

Soares de Alencar Mota C, Ribeiro C, de Araújo GG, de Araújo MB, de Barros Manchado-Gobatto F, Voltarelli FA, de Oliveira CA, Luciano E, de Mello MA - BMC Endocr Disord (2008)

Bottom Line: The necessary overload used was set and periodically readjusted for each rat through effort tests based on the maximal lactate steady state procedure.The area under the serum glucose curve obtained through GTT was always higher in alloxan-treated animals than in controls.At 90 days old the trained controls showed lower HOMA indices than the non-trained controls.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Biosciences, Department of Physical Education, São Paulo State University, Unesp, SP, Brazil. cleciamota@yahoo.com.br

ABSTRACT

Background: Ninety percent of cases of diabetes are of the slowly evolving non-insulin-dependent type, or Type 2 diabetes. Lack of exercise is regarded as one of the main causes of this disorder. In this study we analyzed the effects of physical exercise on glucose homeostasis in adult rats with type 2 diabetes induced by a neonatal injection of alloxan.

Methods: Female Wistar rats aged 6 days were injected with either 250 mg/kg of body weight of alloxan or citrate buffer 0.01 M (controls). After weaning, half of the animals in each group were subjected to physical training adjusted to meet the aerobic-anaerobic metabolic transition by swimming 1 h/day for 5 days a week with weight overloads. The necessary overload used was set and periodically readjusted for each rat through effort tests based on the maximal lactate steady state procedure. When aged 28, 60, 90, and 120 days, the rats underwent glucose tolerance tests (GTT) and their peripheral insulin sensitivity was evaluated using the HOMA index.

Results: The area under the serum glucose curve obtained through GTT was always higher in alloxan-treated animals than in controls. A decrease in this area was observed in trained alloxan-treated rats at 90 and 120 days old compared with non-trained animals. At 90 days old the trained controls showed lower HOMA indices than the non-trained controls.

Conclusion: Neonatal administration of alloxan induced a persistent glucose intolerance in all injected rats, which was successfully counteracted by physical training in the aerobic/anaerobic metabolic transition.

No MeSH data available.


Related in: MedlinePlus

Effort test. Blood lactate concentrations (mmol/L) during the effort tests for determining MLSS of most 28-days-old rats of each group.
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Figure 2: Effort test. Blood lactate concentrations (mmol/L) during the effort tests for determining MLSS of most 28-days-old rats of each group.

Mentions: On the 28th day, all rats were subjected to the described MLSS protocol. MLSS was performed using overloads of 5.0–7.5% of the rats' body weights. The animals did not reach the MLSS using the same overloads within the same group. Figure 2 represents the lactate concentration kinetics during exercise for most animals in each group. In the TC group, 25% of the animals reached the MLSS using overloads of about 5% of their body weight, and their mean lactate concentration was 7.3 ± 2.4 mmol/L; the remaining 75% (Figure 2) reached the MLSS using overloads of 6.5% of their body weight and their mean lactate concentration was 6.0 ± 2.6 mmol/L. In the TA group, 50% of the animals (Figure 2) reached the MLSS using 5.5% of body weight overloads and their mean lactate concentration was 3.8 ± 0.7 mmol/L, while 33.3% of the animals reached the MLSS with 6.0% of body weight overloads and their mean lactate concentration was 6.5 ± 0.6 mmol/L, and 16.7% of the animals reached the MLSS using loads 6.5% of their body weight, with a mean lactate concentration of 10.4 ± 0.6 mmol/L.


Exercise training in the aerobic/anaerobic metabolic transition prevents glucose intolerance in alloxan-treated rats.

Soares de Alencar Mota C, Ribeiro C, de Araújo GG, de Araújo MB, de Barros Manchado-Gobatto F, Voltarelli FA, de Oliveira CA, Luciano E, de Mello MA - BMC Endocr Disord (2008)

Effort test. Blood lactate concentrations (mmol/L) during the effort tests for determining MLSS of most 28-days-old rats of each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2567313&req=5

Figure 2: Effort test. Blood lactate concentrations (mmol/L) during the effort tests for determining MLSS of most 28-days-old rats of each group.
Mentions: On the 28th day, all rats were subjected to the described MLSS protocol. MLSS was performed using overloads of 5.0–7.5% of the rats' body weights. The animals did not reach the MLSS using the same overloads within the same group. Figure 2 represents the lactate concentration kinetics during exercise for most animals in each group. In the TC group, 25% of the animals reached the MLSS using overloads of about 5% of their body weight, and their mean lactate concentration was 7.3 ± 2.4 mmol/L; the remaining 75% (Figure 2) reached the MLSS using overloads of 6.5% of their body weight and their mean lactate concentration was 6.0 ± 2.6 mmol/L. In the TA group, 50% of the animals (Figure 2) reached the MLSS using 5.5% of body weight overloads and their mean lactate concentration was 3.8 ± 0.7 mmol/L, while 33.3% of the animals reached the MLSS with 6.0% of body weight overloads and their mean lactate concentration was 6.5 ± 0.6 mmol/L, and 16.7% of the animals reached the MLSS using loads 6.5% of their body weight, with a mean lactate concentration of 10.4 ± 0.6 mmol/L.

Bottom Line: The necessary overload used was set and periodically readjusted for each rat through effort tests based on the maximal lactate steady state procedure.The area under the serum glucose curve obtained through GTT was always higher in alloxan-treated animals than in controls.At 90 days old the trained controls showed lower HOMA indices than the non-trained controls.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Biosciences, Department of Physical Education, São Paulo State University, Unesp, SP, Brazil. cleciamota@yahoo.com.br

ABSTRACT

Background: Ninety percent of cases of diabetes are of the slowly evolving non-insulin-dependent type, or Type 2 diabetes. Lack of exercise is regarded as one of the main causes of this disorder. In this study we analyzed the effects of physical exercise on glucose homeostasis in adult rats with type 2 diabetes induced by a neonatal injection of alloxan.

Methods: Female Wistar rats aged 6 days were injected with either 250 mg/kg of body weight of alloxan or citrate buffer 0.01 M (controls). After weaning, half of the animals in each group were subjected to physical training adjusted to meet the aerobic-anaerobic metabolic transition by swimming 1 h/day for 5 days a week with weight overloads. The necessary overload used was set and periodically readjusted for each rat through effort tests based on the maximal lactate steady state procedure. When aged 28, 60, 90, and 120 days, the rats underwent glucose tolerance tests (GTT) and their peripheral insulin sensitivity was evaluated using the HOMA index.

Results: The area under the serum glucose curve obtained through GTT was always higher in alloxan-treated animals than in controls. A decrease in this area was observed in trained alloxan-treated rats at 90 and 120 days old compared with non-trained animals. At 90 days old the trained controls showed lower HOMA indices than the non-trained controls.

Conclusion: Neonatal administration of alloxan induced a persistent glucose intolerance in all injected rats, which was successfully counteracted by physical training in the aerobic/anaerobic metabolic transition.

No MeSH data available.


Related in: MedlinePlus