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Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.

Fuentes EO, Leemhuis J, Stark GB, Lang EM - J Brachial Plex Peripher Nerve Inj (2008)

Bottom Line: The longest neurite per neuron were measured and compared.Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells.When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. ckitofdr@gmail.com

ABSTRACT

Background: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.

Methods: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.

No MeSH data available.


Related in: MedlinePlus

Histogram comparing neurite lengths of neurons in contact with Schwann cells after incubation with C3 FT, Y27632 and H1152. *** p ≤ 0.001.
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Figure 4: Histogram comparing neurite lengths of neurons in contact with Schwann cells after incubation with C3 FT, Y27632 and H1152. *** p ≤ 0.001.

Mentions: Next the length of the longest neurite of the neurons, which were in direct contact with SC were studied. To this end 144 control, 141 C3 FT, 138 Y27632 and 137 H1152-treated neurons were analysed. Interestingly, neurons in the C3 FT, Y27632 and H1152 groups had on average longer neurites than in the control group (Figure. 4). The average neurite lengths obtained were 190.4 μm (SE ± 8.3) in the controls, 213.8 μm (SE ± 9.5) in the C3 FT group, 259.7 μm (SE ± 9.1) in the Y27632 group and 244.4 μm (SE ± 11.4) in the H1152 group. However, only the neurite lengths in the Y27632 and H1152 groups were significantly longer than controls (p < 0.001 and p = 0.001 respectively). There was no significant difference between the C3 FT, Y27632 and H1152 groups (Figure. 4).


Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.

Fuentes EO, Leemhuis J, Stark GB, Lang EM - J Brachial Plex Peripher Nerve Inj (2008)

Histogram comparing neurite lengths of neurons in contact with Schwann cells after incubation with C3 FT, Y27632 and H1152. *** p ≤ 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2567309&req=5

Figure 4: Histogram comparing neurite lengths of neurons in contact with Schwann cells after incubation with C3 FT, Y27632 and H1152. *** p ≤ 0.001.
Mentions: Next the length of the longest neurite of the neurons, which were in direct contact with SC were studied. To this end 144 control, 141 C3 FT, 138 Y27632 and 137 H1152-treated neurons were analysed. Interestingly, neurons in the C3 FT, Y27632 and H1152 groups had on average longer neurites than in the control group (Figure. 4). The average neurite lengths obtained were 190.4 μm (SE ± 8.3) in the controls, 213.8 μm (SE ± 9.5) in the C3 FT group, 259.7 μm (SE ± 9.1) in the Y27632 group and 244.4 μm (SE ± 11.4) in the H1152 group. However, only the neurite lengths in the Y27632 and H1152 groups were significantly longer than controls (p < 0.001 and p = 0.001 respectively). There was no significant difference between the C3 FT, Y27632 and H1152 groups (Figure. 4).

Bottom Line: The longest neurite per neuron were measured and compared.Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells.When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. ckitofdr@gmail.com

ABSTRACT

Background: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.

Methods: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.

No MeSH data available.


Related in: MedlinePlus