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Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.

Fuentes EO, Leemhuis J, Stark GB, Lang EM - J Brachial Plex Peripher Nerve Inj (2008)

Bottom Line: The longest neurite per neuron were measured and compared.Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells.When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. ckitofdr@gmail.com

ABSTRACT

Background: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.

Methods: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.

No MeSH data available.


Related in: MedlinePlus

Neurite length of purified DRG neurons after an overnight incubation with 10 ng/ml NGF followed by 8 h incubation with C3 FT, Y27632 and H1152.
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Figure 2: Neurite length of purified DRG neurons after an overnight incubation with 10 ng/ml NGF followed by 8 h incubation with C3 FT, Y27632 and H1152.

Mentions: Dissociated DRG neuron cultures incubated overnight with 10 ng/ml of NGF followed by an 8 h incubation with C3 fusion toxin and the ROCK inhibitors Y27632 and H1152 showed an increase in the average length of their longest neurites when compared to the control group (medium only). The average maximal neurite lengths were in the controls 110.1 μm (SE ± 7.8) in the C3FT group 162.4 μm (SE ± 10.4), in the Y27632 group 167.8 μm (SE ± 12.3), and 185.4 μm (SE ± 16.6) in the H1152 group. All groups showed significantly longer neurites than the control group (p < 0.001). There was no significant difference between the three treatment groups (Figure. 2).


Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.

Fuentes EO, Leemhuis J, Stark GB, Lang EM - J Brachial Plex Peripher Nerve Inj (2008)

Neurite length of purified DRG neurons after an overnight incubation with 10 ng/ml NGF followed by 8 h incubation with C3 FT, Y27632 and H1152.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2567309&req=5

Figure 2: Neurite length of purified DRG neurons after an overnight incubation with 10 ng/ml NGF followed by 8 h incubation with C3 FT, Y27632 and H1152.
Mentions: Dissociated DRG neuron cultures incubated overnight with 10 ng/ml of NGF followed by an 8 h incubation with C3 fusion toxin and the ROCK inhibitors Y27632 and H1152 showed an increase in the average length of their longest neurites when compared to the control group (medium only). The average maximal neurite lengths were in the controls 110.1 μm (SE ± 7.8) in the C3FT group 162.4 μm (SE ± 10.4), in the Y27632 group 167.8 μm (SE ± 12.3), and 185.4 μm (SE ± 16.6) in the H1152 group. All groups showed significantly longer neurites than the control group (p < 0.001). There was no significant difference between the three treatment groups (Figure. 2).

Bottom Line: The longest neurite per neuron were measured and compared.Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells.When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. ckitofdr@gmail.com

ABSTRACT

Background: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.

Methods: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.

No MeSH data available.


Related in: MedlinePlus