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Iota-Carrageenan is a potent inhibitor of rhinovirus infection.

Grassauer A, Weinmuellner R, Meier C, Pretsch A, Prieschl-Grassauer E, Unger H - Virol. J. (2008)

Bottom Line: Despite significant efforts, no anti-viral agent is approved for the prevention or treatment of HRV-infection.In addition, Iota-Carrageenan effectively prevents the replication of HRV1A, HRV2, HRV8, HRV14, HRV16, HRV83 and HRV84 in primary human nasal epithelial cells in culture.Since HRV infections predominately occur in the nasal cavity and the upper respiratory tract, a targeted treatment with a product containing Iota-Carrageenan is conceivable.

View Article: PubMed Central - HTML - PubMed

Affiliation: Marinomed Biotechnologie GmbH, Veterinaerplatz 1/HA, A-1210 Vienna, Austria. andreas.grassauer@marinomed.com

ABSTRACT

Background: Human rhinoviruses (HRVs) are the predominant cause of common cold. In addition, HRVs are implicated in the worsening of COPD and asthma, as well as the loss of lung transplants. Despite significant efforts, no anti-viral agent is approved for the prevention or treatment of HRV-infection.

Results: In this study we demonstrate that Iota-Carrageenan, a sulphated polysaccharide derived from red seaweed, is a potent anti-rhinoviral substance in-vitro. Iota-Carrageenan reduces HRV growth and inhibits the virus induced cythopathic effect of infected HeLa cells. In addition, Iota-Carrageenan effectively prevents the replication of HRV1A, HRV2, HRV8, HRV14, HRV16, HRV83 and HRV84 in primary human nasal epithelial cells in culture. The data suggest that Iota-Carrageenan acts primarily by preventing the binding or the entry of virions into the cells.

Conclusion: Since HRV infections predominately occur in the nasal cavity and the upper respiratory tract, a targeted treatment with a product containing Iota-Carrageenan is conceivable. Clinical trials are needed to determine whether Iota-Carrageenan-based products are effective in the treatment or prophylaxis of HRV infections.

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Effect of Iota-carageenan on HRV2 infected human nasal epithelial cells. A. Preincubation of virus with polymer. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Iota-Carrageenan at the concentration indicated at the x-axis. 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated was added. B. Treatment with polymer after infection. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell). 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated at the x-axis was added. Viral titers in the supernatants of infected cells were determined after 48 h by TCID50 assay on HeLa cells (y-axis). Bars represent means of four parallel experiments, standard deviation is indicated.
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Figure 5: Effect of Iota-carageenan on HRV2 infected human nasal epithelial cells. A. Preincubation of virus with polymer. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Iota-Carrageenan at the concentration indicated at the x-axis. 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated was added. B. Treatment with polymer after infection. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell). 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated at the x-axis was added. Viral titers in the supernatants of infected cells were determined after 48 h by TCID50 assay on HeLa cells (y-axis). Bars represent means of four parallel experiments, standard deviation is indicated.

Mentions: In order to study whether the activity against HRV is a tissue culture phenomenon an experiment with human nasal epithelial cells (HNep) was conducted. HNep cells were grown in 24-well plates. The infection with HRV2 (0,1 TCID50/cell) was carried out in the presence or absence of Iota-Carrageenan and 30 minutes post infection medium containing polymer in the range of 0,2 μg/ml to 500 μg/ml was added. After 48 hours analysis of viral titers in the supernatants of infected cells revealed that HRV2 replicates to titers of approximately 107 TCID50/ml on HNep cells. The viral titer was below the detection limit of 102 TCID50/ml when 55 μg/ml of Iota-Carrageenan was already present during the infection (Figure 5A). When the infection was done in the absence of Iota-Carrageenan a concentration of 500 μg/ml was needed to reduce the viral replication below the detection limit (Figure 5B). However, in both cases a significant reduction in the viral titer was observed when the polymer concentration was at least 2 μg/ml. This result shows that Iota-Carrageenan inhibits replication of HRV2 on HNep cells.


Iota-Carrageenan is a potent inhibitor of rhinovirus infection.

Grassauer A, Weinmuellner R, Meier C, Pretsch A, Prieschl-Grassauer E, Unger H - Virol. J. (2008)

Effect of Iota-carageenan on HRV2 infected human nasal epithelial cells. A. Preincubation of virus with polymer. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Iota-Carrageenan at the concentration indicated at the x-axis. 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated was added. B. Treatment with polymer after infection. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell). 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated at the x-axis was added. Viral titers in the supernatants of infected cells were determined after 48 h by TCID50 assay on HeLa cells (y-axis). Bars represent means of four parallel experiments, standard deviation is indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC2562995&req=5

Figure 5: Effect of Iota-carageenan on HRV2 infected human nasal epithelial cells. A. Preincubation of virus with polymer. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Iota-Carrageenan at the concentration indicated at the x-axis. 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated was added. B. Treatment with polymer after infection. HNep cells were grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell). 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated at the x-axis was added. Viral titers in the supernatants of infected cells were determined after 48 h by TCID50 assay on HeLa cells (y-axis). Bars represent means of four parallel experiments, standard deviation is indicated.
Mentions: In order to study whether the activity against HRV is a tissue culture phenomenon an experiment with human nasal epithelial cells (HNep) was conducted. HNep cells were grown in 24-well plates. The infection with HRV2 (0,1 TCID50/cell) was carried out in the presence or absence of Iota-Carrageenan and 30 minutes post infection medium containing polymer in the range of 0,2 μg/ml to 500 μg/ml was added. After 48 hours analysis of viral titers in the supernatants of infected cells revealed that HRV2 replicates to titers of approximately 107 TCID50/ml on HNep cells. The viral titer was below the detection limit of 102 TCID50/ml when 55 μg/ml of Iota-Carrageenan was already present during the infection (Figure 5A). When the infection was done in the absence of Iota-Carrageenan a concentration of 500 μg/ml was needed to reduce the viral replication below the detection limit (Figure 5B). However, in both cases a significant reduction in the viral titer was observed when the polymer concentration was at least 2 μg/ml. This result shows that Iota-Carrageenan inhibits replication of HRV2 on HNep cells.

Bottom Line: Despite significant efforts, no anti-viral agent is approved for the prevention or treatment of HRV-infection.In addition, Iota-Carrageenan effectively prevents the replication of HRV1A, HRV2, HRV8, HRV14, HRV16, HRV83 and HRV84 in primary human nasal epithelial cells in culture.Since HRV infections predominately occur in the nasal cavity and the upper respiratory tract, a targeted treatment with a product containing Iota-Carrageenan is conceivable.

View Article: PubMed Central - HTML - PubMed

Affiliation: Marinomed Biotechnologie GmbH, Veterinaerplatz 1/HA, A-1210 Vienna, Austria. andreas.grassauer@marinomed.com

ABSTRACT

Background: Human rhinoviruses (HRVs) are the predominant cause of common cold. In addition, HRVs are implicated in the worsening of COPD and asthma, as well as the loss of lung transplants. Despite significant efforts, no anti-viral agent is approved for the prevention or treatment of HRV-infection.

Results: In this study we demonstrate that Iota-Carrageenan, a sulphated polysaccharide derived from red seaweed, is a potent anti-rhinoviral substance in-vitro. Iota-Carrageenan reduces HRV growth and inhibits the virus induced cythopathic effect of infected HeLa cells. In addition, Iota-Carrageenan effectively prevents the replication of HRV1A, HRV2, HRV8, HRV14, HRV16, HRV83 and HRV84 in primary human nasal epithelial cells in culture. The data suggest that Iota-Carrageenan acts primarily by preventing the binding or the entry of virions into the cells.

Conclusion: Since HRV infections predominately occur in the nasal cavity and the upper respiratory tract, a targeted treatment with a product containing Iota-Carrageenan is conceivable. Clinical trials are needed to determine whether Iota-Carrageenan-based products are effective in the treatment or prophylaxis of HRV infections.

Show MeSH
Related in: MedlinePlus