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Mix and measure fluorescence screening for selective quadruplex binders.

Paramasivan S, Bolton PH - Nucleic Acids Res. (2008)

Bottom Line: The successful targeting of a particular quadruplex structure requires distinguishing that structure from all of the other quadruplex structures that may be present.The screening is based on observing the increase or decrease in the fluorescence of the reporter molecules.The selectivity of a set of test molecules has been determined by this approach.

View Article: PubMed Central - PubMed

Affiliation: Chemistry Department, Wesleyan University, Middletown, CT 06459, USA.

ABSTRACT
The human genome contains thousands of regions, including that of the telomere, that have the potential to form quadruplex structures. Many of these regions are potential targets for therapeutic intervention. There are many different folding patterns for quadruplex DNAs and the loops exhibit much more variation than do the quartets. The successful targeting of a particular quadruplex structure requires distinguishing that structure from all of the other quadruplex structures that may be present. A mix and measure fluorescent screening method has been developed, that utilizes multiple reporter molecules that bind to different features of quadruplex DNA. The reporter molecules are used in combination with DNAs that have a variety of quadruplex structures. The screening is based on observing the increase or decrease in the fluorescence of the reporter molecules. The selectivity of a set of test molecules has been determined by this approach.

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The effect of the addition of NMM has on the CD spectra of DODC and DTDC in the presence of the indicated DNAs. In the case of d(GGTTGGTGTGGTTGG) NMM exhibits induced CD when DODC is present.
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Figure 4: The effect of the addition of NMM has on the CD spectra of DODC and DTDC in the presence of the indicated DNAs. In the case of d(GGTTGGTGTGGTTGG) NMM exhibits induced CD when DODC is present.

Mentions: The effects of NMM on the fluorescence and induced CD of DODC and DTDC were examined to determine the extent to which the reporter molecules compete with one another. The induced CD of DODC and DTDC were monitored as a function of NMM concentration and selected results are shown in Figure 4.Figure 4.


Mix and measure fluorescence screening for selective quadruplex binders.

Paramasivan S, Bolton PH - Nucleic Acids Res. (2008)

The effect of the addition of NMM has on the CD spectra of DODC and DTDC in the presence of the indicated DNAs. In the case of d(GGTTGGTGTGGTTGG) NMM exhibits induced CD when DODC is present.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2553591&req=5

Figure 4: The effect of the addition of NMM has on the CD spectra of DODC and DTDC in the presence of the indicated DNAs. In the case of d(GGTTGGTGTGGTTGG) NMM exhibits induced CD when DODC is present.
Mentions: The effects of NMM on the fluorescence and induced CD of DODC and DTDC were examined to determine the extent to which the reporter molecules compete with one another. The induced CD of DODC and DTDC were monitored as a function of NMM concentration and selected results are shown in Figure 4.Figure 4.

Bottom Line: The successful targeting of a particular quadruplex structure requires distinguishing that structure from all of the other quadruplex structures that may be present.The screening is based on observing the increase or decrease in the fluorescence of the reporter molecules.The selectivity of a set of test molecules has been determined by this approach.

View Article: PubMed Central - PubMed

Affiliation: Chemistry Department, Wesleyan University, Middletown, CT 06459, USA.

ABSTRACT
The human genome contains thousands of regions, including that of the telomere, that have the potential to form quadruplex structures. Many of these regions are potential targets for therapeutic intervention. There are many different folding patterns for quadruplex DNAs and the loops exhibit much more variation than do the quartets. The successful targeting of a particular quadruplex structure requires distinguishing that structure from all of the other quadruplex structures that may be present. A mix and measure fluorescent screening method has been developed, that utilizes multiple reporter molecules that bind to different features of quadruplex DNA. The reporter molecules are used in combination with DNAs that have a variety of quadruplex structures. The screening is based on observing the increase or decrease in the fluorescence of the reporter molecules. The selectivity of a set of test molecules has been determined by this approach.

Show MeSH