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Control of the rate of evaporation in protein crystallization by the 'microbatch under oil' method.

Brumshtein B, Greenblatt HM, Futerman AH, Silman I, Sussman JL - J Appl Crystallogr (2008)

Bottom Line: Microbatch crystallization under oil is a powerful procedure for obtaining protein crystals.Using this method, aqueous protein solutions are dispensed under liquid oil, and water evaporates through the layer of oil, with a concomitant increase in the concentrations of both protein and precipitant until the nucleation point is reached.A technique is presented for regulating the rate of water evaporation, which permits fine tuning of the crystallization conditions as well as preventing complete desiccation of the drops in the microbatch crystallization trays.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Microbatch crystallization under oil is a powerful procedure for obtaining protein crystals. Using this method, aqueous protein solutions are dispensed under liquid oil, and water evaporates through the layer of oil, with a concomitant increase in the concentrations of both protein and precipitant until the nucleation point is reached. A technique is presented for regulating the rate of water evaporation, which permits fine tuning of the crystallization conditions as well as preventing complete desiccation of the drops in the microbatch crystallization trays.

No MeSH data available.


Douglas Vapor Batch hydrophobic crystallization plate. Crystallization droplets are dispensed under oil into the wells in the central part of the plate. The humidity within the tray above the oil-covered droplets is determined by the vapor pressure of the liquid in the reservoirs.
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fig1: Douglas Vapor Batch hydrophobic crystallization plate. Crystallization droplets are dispensed under oil into the wells in the central part of the plate. The humidity within the tray above the oil-covered droplets is determined by the vapor pressure of the liquid in the reservoirs.

Mentions: The key innovation is to include an aqueous reservoir within the microbatch crystallization tray (Fig. 1 ▶). By making a suitable choice of the vapor pressure of the solution within this reservoir, it is possible both to control the rate of evaporation from the microbatch droplets and to eliminate the risk of their drying out.


Control of the rate of evaporation in protein crystallization by the 'microbatch under oil' method.

Brumshtein B, Greenblatt HM, Futerman AH, Silman I, Sussman JL - J Appl Crystallogr (2008)

Douglas Vapor Batch hydrophobic crystallization plate. Crystallization droplets are dispensed under oil into the wells in the central part of the plate. The humidity within the tray above the oil-covered droplets is determined by the vapor pressure of the liquid in the reservoirs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2553557&req=5

fig1: Douglas Vapor Batch hydrophobic crystallization plate. Crystallization droplets are dispensed under oil into the wells in the central part of the plate. The humidity within the tray above the oil-covered droplets is determined by the vapor pressure of the liquid in the reservoirs.
Mentions: The key innovation is to include an aqueous reservoir within the microbatch crystallization tray (Fig. 1 ▶). By making a suitable choice of the vapor pressure of the solution within this reservoir, it is possible both to control the rate of evaporation from the microbatch droplets and to eliminate the risk of their drying out.

Bottom Line: Microbatch crystallization under oil is a powerful procedure for obtaining protein crystals.Using this method, aqueous protein solutions are dispensed under liquid oil, and water evaporates through the layer of oil, with a concomitant increase in the concentrations of both protein and precipitant until the nucleation point is reached.A technique is presented for regulating the rate of water evaporation, which permits fine tuning of the crystallization conditions as well as preventing complete desiccation of the drops in the microbatch crystallization trays.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Microbatch crystallization under oil is a powerful procedure for obtaining protein crystals. Using this method, aqueous protein solutions are dispensed under liquid oil, and water evaporates through the layer of oil, with a concomitant increase in the concentrations of both protein and precipitant until the nucleation point is reached. A technique is presented for regulating the rate of water evaporation, which permits fine tuning of the crystallization conditions as well as preventing complete desiccation of the drops in the microbatch crystallization trays.

No MeSH data available.