Limits...
The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.


Microscopically, histological detection showed new bone tissue formed by tissue-engineered bone membrane, and new bone tissue arranged irregularly around a lot of porosity, in which there were primary vessels and biodegraded leftovers of SIS. a HE staining (×100); b Masson staining (×100)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2553429&req=5

Fig7: Microscopically, histological detection showed new bone tissue formed by tissue-engineered bone membrane, and new bone tissue arranged irregularly around a lot of porosity, in which there were primary vessels and biodegraded leftovers of SIS. a HE staining (×100); b Masson staining (×100)

Mentions: Histological sections showed fibrous connective tissue and SIS degraded remnants filling in the region of defect repaired with SIS alone with no discernable osseous tissue. In comparison, newly formed osseous tissue was detected in the defect region treated with M1 or M2. The new bone tissue grew around irregular spherical porosities, which were occupied partially by medullary cavities or vessels in addition to degraded remnants of SIS (Fig. 7). No inflammatory cells were identified in all sections from all groups. Fig. 7


The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

Microscopically, histological detection showed new bone tissue formed by tissue-engineered bone membrane, and new bone tissue arranged irregularly around a lot of porosity, in which there were primary vessels and biodegraded leftovers of SIS. a HE staining (×100); b Masson staining (×100)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2553429&req=5

Fig7: Microscopically, histological detection showed new bone tissue formed by tissue-engineered bone membrane, and new bone tissue arranged irregularly around a lot of porosity, in which there were primary vessels and biodegraded leftovers of SIS. a HE staining (×100); b Masson staining (×100)
Mentions: Histological sections showed fibrous connective tissue and SIS degraded remnants filling in the region of defect repaired with SIS alone with no discernable osseous tissue. In comparison, newly formed osseous tissue was detected in the defect region treated with M1 or M2. The new bone tissue grew around irregular spherical porosities, which were occupied partially by medullary cavities or vessels in addition to degraded remnants of SIS (Fig. 7). No inflammatory cells were identified in all sections from all groups. Fig. 7

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.