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The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.


Representative rabbit radial radiographs, 4 weeks after the implantation operation (red line shows the region of radial bone defect). a The segmental bone defect treated with tissue-engineered bone membrane M1(MSC + SIS) showed bone repairing. b The critical size bone defect treated with tissue-engineered bone membrane M2 (osteoblast induced from MSC + SIS) also showed bone repairing. c The segmental bone defect treated with SIS is empty under X-ray detection. These radiographs confirmed that critical bone defect can be repaired by tissue-engineered bone membrane, either constructed with seeding cells of allogenic MSCs or osteogenetic induced allogenic MSCs
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Fig6: Representative rabbit radial radiographs, 4 weeks after the implantation operation (red line shows the region of radial bone defect). a The segmental bone defect treated with tissue-engineered bone membrane M1(MSC + SIS) showed bone repairing. b The critical size bone defect treated with tissue-engineered bone membrane M2 (osteoblast induced from MSC + SIS) also showed bone repairing. c The segmental bone defect treated with SIS is empty under X-ray detection. These radiographs confirmed that critical bone defect can be repaired by tissue-engineered bone membrane, either constructed with seeding cells of allogenic MSCs or osteogenetic induced allogenic MSCs

Mentions: All defects were clearly discernible with no bone formation observed after 4 weeks at sites treated only with SIS (Fig. 6). This contrasts with the radio-opaque tissue observed at the same time at all sites treated with the M1 or M2 samples. The observed new bone density was lower than that at the edges of the defect and of cortical bone but higher than that of the olecranon process and medullary cavity. The defect treated with M1 or M2 were almost completely bridged and occupied by a newly formed, uniform density callous. Neither hypertrophy nor atrophy was observed on both ends of the bone defect.Fig. 6


The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

Representative rabbit radial radiographs, 4 weeks after the implantation operation (red line shows the region of radial bone defect). a The segmental bone defect treated with tissue-engineered bone membrane M1(MSC + SIS) showed bone repairing. b The critical size bone defect treated with tissue-engineered bone membrane M2 (osteoblast induced from MSC + SIS) also showed bone repairing. c The segmental bone defect treated with SIS is empty under X-ray detection. These radiographs confirmed that critical bone defect can be repaired by tissue-engineered bone membrane, either constructed with seeding cells of allogenic MSCs or osteogenetic induced allogenic MSCs
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2553429&req=5

Fig6: Representative rabbit radial radiographs, 4 weeks after the implantation operation (red line shows the region of radial bone defect). a The segmental bone defect treated with tissue-engineered bone membrane M1(MSC + SIS) showed bone repairing. b The critical size bone defect treated with tissue-engineered bone membrane M2 (osteoblast induced from MSC + SIS) also showed bone repairing. c The segmental bone defect treated with SIS is empty under X-ray detection. These radiographs confirmed that critical bone defect can be repaired by tissue-engineered bone membrane, either constructed with seeding cells of allogenic MSCs or osteogenetic induced allogenic MSCs
Mentions: All defects were clearly discernible with no bone formation observed after 4 weeks at sites treated only with SIS (Fig. 6). This contrasts with the radio-opaque tissue observed at the same time at all sites treated with the M1 or M2 samples. The observed new bone density was lower than that at the edges of the defect and of cortical bone but higher than that of the olecranon process and medullary cavity. The defect treated with M1 or M2 were almost completely bridged and occupied by a newly formed, uniform density callous. Neither hypertrophy nor atrophy was observed on both ends of the bone defect.Fig. 6

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.