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The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.


SEM picture of the surface of SIS. Manufactured SIS show a velvet-like, broad surface with numerous fibre bundles, which form a variety of small, irregular porosities
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Fig4: SEM picture of the surface of SIS. Manufactured SIS show a velvet-like, broad surface with numerous fibre bundles, which form a variety of small, irregular porosities

Mentions: The SIS scaffold we manufactured was a white coloured membrane-like structure with a 100 ± 20 μm thickness and a flexible texture similar to natural bone membrane (Fig. 3). SEM observation revealed that the SIS had a velvet-like surface and irregular porous membrane structure constituted by mesh-like collagen fibre bundles (Fig. 4). The characteristics (thickness and porosity) of SIS were such that it was feasible to fabricate a tissue-engineered bone membrane implant. This scaffold not only provided a large cellular adherence area but also had the potential to allow the seeded cells to survive through diffusion of the nutrients from outside the scaffold owing to a thickness of less than 0.5 mm [11]. SEM observation of the surface of the tissue-engineered bone membrane showed that the seeded cells adhered to the SIS extensively with almost all of the scaffold surface occupied (Fig. 5).Fig. 3


The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation.

Zhao L, Zhao JL, Wan L, Wang SK - Strategies Trauma Limb Reconstr (2008)

SEM picture of the surface of SIS. Manufactured SIS show a velvet-like, broad surface with numerous fibre bundles, which form a variety of small, irregular porosities
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2553429&req=5

Fig4: SEM picture of the surface of SIS. Manufactured SIS show a velvet-like, broad surface with numerous fibre bundles, which form a variety of small, irregular porosities
Mentions: The SIS scaffold we manufactured was a white coloured membrane-like structure with a 100 ± 20 μm thickness and a flexible texture similar to natural bone membrane (Fig. 3). SEM observation revealed that the SIS had a velvet-like surface and irregular porous membrane structure constituted by mesh-like collagen fibre bundles (Fig. 4). The characteristics (thickness and porosity) of SIS were such that it was feasible to fabricate a tissue-engineered bone membrane implant. This scaffold not only provided a large cellular adherence area but also had the potential to allow the seeded cells to survive through diffusion of the nutrients from outside the scaffold owing to a thickness of less than 0.5 mm [11]. SEM observation of the surface of the tissue-engineered bone membrane showed that the seeded cells adhered to the SIS extensively with almost all of the scaffold surface occupied (Fig. 5).Fig. 3

Bottom Line: This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone.There was no attempt to bridge in the bone defect treated by SIS.Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, 80 CuiYingMen, ChengGuan District, 730030, Lanzhou City, People's Republic of China, bonezl@sina.com.cn.

ABSTRACT
The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

No MeSH data available.