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Losses of expression of the antigens A, Lea and Lex and over-expression of Ley in carcinomas and HG-SIL of the uterine cervix.

Moro-Rodríguez E, Alvarez-Fernández E - Diagn Pathol (2008)

Bottom Line: With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups.There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens.These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Area de Patología, Universidad Rey Juan Carlos, Madrid, Spain. jemoro@uninet.edu

ABSTRACT

Background: The glycosylation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades.

Objective: To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix.

Methods: A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas) have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8) for precursor chains, blood-group ABH and Lewis group Le(a), Le(b), Le(x), and Le(y), and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16.

Results: In the invasive cervical carcinomas, we observed a loss of expression of the Le(x) antigen (p < 0.01). With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups. There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms.

Conclusion: These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.

No MeSH data available.


Related in: MedlinePlus

Up: Extension of CIN III in the pseudoglands that show a cellularity with heterogenous expression and diminution of the expression of MoAb BG2 (Blood Group A). Down: Detail of the same lesion where an abrupt cut of expression of MoAb BG2 is observed. In addition the expression of this Ab in the endothelial lining of small capillares can be appreciated like internal control.
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Figure 6: Up: Extension of CIN III in the pseudoglands that show a cellularity with heterogenous expression and diminution of the expression of MoAb BG2 (Blood Group A). Down: Detail of the same lesion where an abrupt cut of expression of MoAb BG2 is observed. In addition the expression of this Ab in the endothelial lining of small capillares can be appreciated like internal control.

Mentions: All the cases with A phenotype expressed this antigenic determinant in high-degree lesions, but with a certain tendency towards a heterogeneous pattern of expression and weaker stains. 5 out of 9 cases (55,5%), positive for BG2 in normal epithelia, lost their expression in areas of HG-SIL, some with sharp losses of expression and without a gradual transition from the positive fields. Concerning the B antigenic determinant, we found four cases that expressed this antigen (4/16 25%). Three of these cases showed heterogeneous patterns. Two were patients from the O group and another patient belonged to the AB group. These four cases also expressed the same antigen in the squamous epithelium and, therefore, we cannot consider them aberrant expressions. Among these cases, we also observed focal losses of antigenic expression. (Figure 5, Figure 6)


Losses of expression of the antigens A, Lea and Lex and over-expression of Ley in carcinomas and HG-SIL of the uterine cervix.

Moro-Rodríguez E, Alvarez-Fernández E - Diagn Pathol (2008)

Up: Extension of CIN III in the pseudoglands that show a cellularity with heterogenous expression and diminution of the expression of MoAb BG2 (Blood Group A). Down: Detail of the same lesion where an abrupt cut of expression of MoAb BG2 is observed. In addition the expression of this Ab in the endothelial lining of small capillares can be appreciated like internal control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2551588&req=5

Figure 6: Up: Extension of CIN III in the pseudoglands that show a cellularity with heterogenous expression and diminution of the expression of MoAb BG2 (Blood Group A). Down: Detail of the same lesion where an abrupt cut of expression of MoAb BG2 is observed. In addition the expression of this Ab in the endothelial lining of small capillares can be appreciated like internal control.
Mentions: All the cases with A phenotype expressed this antigenic determinant in high-degree lesions, but with a certain tendency towards a heterogeneous pattern of expression and weaker stains. 5 out of 9 cases (55,5%), positive for BG2 in normal epithelia, lost their expression in areas of HG-SIL, some with sharp losses of expression and without a gradual transition from the positive fields. Concerning the B antigenic determinant, we found four cases that expressed this antigen (4/16 25%). Three of these cases showed heterogeneous patterns. Two were patients from the O group and another patient belonged to the AB group. These four cases also expressed the same antigen in the squamous epithelium and, therefore, we cannot consider them aberrant expressions. Among these cases, we also observed focal losses of antigenic expression. (Figure 5, Figure 6)

Bottom Line: With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups.There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens.These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Area de Patología, Universidad Rey Juan Carlos, Madrid, Spain. jemoro@uninet.edu

ABSTRACT

Background: The glycosylation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades.

Objective: To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix.

Methods: A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas) have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8) for precursor chains, blood-group ABH and Lewis group Le(a), Le(b), Le(x), and Le(y), and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16.

Results: In the invasive cervical carcinomas, we observed a loss of expression of the Le(x) antigen (p < 0.01). With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups. There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms.

Conclusion: These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.

No MeSH data available.


Related in: MedlinePlus