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TLR3 and TLR4 expression in healthy and diseased human endometrium.

Allhorn S, Böing C, Koch AA, Kimmig R, Gashaw I - Reprod. Biol. Endocrinol. (2008)

Bottom Line: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens.In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls.Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Anatomy II, University of Duisburg-Essen, Essen, Germany.

ABSTRACT

Background: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens. TLRs are expressed in the human endometrium and their regulation might be crucial for the pathogenesis of endometrial diseases.

Methods: TLR3 and TLR4 expression was investigated during the menstrual cycle and in postmenopausal endometrium considering peritoneal endometriosis, hyperplasia, and endometrial adenocarcinoma specimens (grade 1 to 3). The expression studies applied quantitative RT-PCR and immunolabelling of both proteins.

Results: TLR3 and TLR4 proteins were mostly localised to the glandular and luminal epithelium. In addition, TLR4 was present on endometrial dendritic cells, monocytes and macrophages. TLR3 and TLR4 mRNA levels did not show significant changes during the menstrual cycle. In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls. Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression. Endometrial hyperplasia and adenocarcinoma revealed significantly reduced receptor levels when compared with postmenopausal controls. The lowest TLR expression levels were determined in poor differentiated carcinoma (grade 3).

Conclusion: Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.

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Related in: MedlinePlus

TLR3 and TLR4 mRNA expression is decreased in endometrial adenocarcinoma. (A-B) Columns indicate mean TLR3 (A) and TLR4 (B) mRNA levels from postmenopausal patients (PMP, n = 8), and those diagnosed with endometrial hyperplasia (HP, n = 10) and endometrial carcinoma (EnCa, n = 16). (C-D) TLR3 (C) and TLR4 (D) mRNA expression in different carcinoma grades compared to postmenopausal controls and hyperplastic endometrium: G1 (n = 5), G2 (n = 6) and G3 (n = 5). Error bars represent the standard deviation of the mean. * P < 0.05; ** P < 0.01.
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Figure 5: TLR3 and TLR4 mRNA expression is decreased in endometrial adenocarcinoma. (A-B) Columns indicate mean TLR3 (A) and TLR4 (B) mRNA levels from postmenopausal patients (PMP, n = 8), and those diagnosed with endometrial hyperplasia (HP, n = 10) and endometrial carcinoma (EnCa, n = 16). (C-D) TLR3 (C) and TLR4 (D) mRNA expression in different carcinoma grades compared to postmenopausal controls and hyperplastic endometrium: G1 (n = 5), G2 (n = 6) and G3 (n = 5). Error bars represent the standard deviation of the mean. * P < 0.05; ** P < 0.01.

Mentions: TLR3 and TLR4 mRNA abundance in healthy postmenopausal tissues is similar to those found during the menstrual cycle. In postmenopausal controls, TLR4 mRNA levels were higher than those for TLR3 (P < 0.05, figure 5). TLR3 and TLR4 mRNA expression varied significantly between control, hyperplasia and endometrial adenocarcinoma samples (Kruskal-Wallis test, P < 0.01). For both receptors, we observe a significant decrease in mRNA abundance in endometrial hyperplasia and adenocarcinoma samples, when compared to postmenopausal endometrium (P < 0.05; figure 5A–B). In undifferentiated G3 carcinoma, TLR3 and TLR4 mRNA levels were significantly lower than in postmenopausal controls (P < 0.01) and in hyperplasic endometrial tissues (P < 0.05, figure 5C–D).


TLR3 and TLR4 expression in healthy and diseased human endometrium.

Allhorn S, Böing C, Koch AA, Kimmig R, Gashaw I - Reprod. Biol. Endocrinol. (2008)

TLR3 and TLR4 mRNA expression is decreased in endometrial adenocarcinoma. (A-B) Columns indicate mean TLR3 (A) and TLR4 (B) mRNA levels from postmenopausal patients (PMP, n = 8), and those diagnosed with endometrial hyperplasia (HP, n = 10) and endometrial carcinoma (EnCa, n = 16). (C-D) TLR3 (C) and TLR4 (D) mRNA expression in different carcinoma grades compared to postmenopausal controls and hyperplastic endometrium: G1 (n = 5), G2 (n = 6) and G3 (n = 5). Error bars represent the standard deviation of the mean. * P < 0.05; ** P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2543020&req=5

Figure 5: TLR3 and TLR4 mRNA expression is decreased in endometrial adenocarcinoma. (A-B) Columns indicate mean TLR3 (A) and TLR4 (B) mRNA levels from postmenopausal patients (PMP, n = 8), and those diagnosed with endometrial hyperplasia (HP, n = 10) and endometrial carcinoma (EnCa, n = 16). (C-D) TLR3 (C) and TLR4 (D) mRNA expression in different carcinoma grades compared to postmenopausal controls and hyperplastic endometrium: G1 (n = 5), G2 (n = 6) and G3 (n = 5). Error bars represent the standard deviation of the mean. * P < 0.05; ** P < 0.01.
Mentions: TLR3 and TLR4 mRNA abundance in healthy postmenopausal tissues is similar to those found during the menstrual cycle. In postmenopausal controls, TLR4 mRNA levels were higher than those for TLR3 (P < 0.05, figure 5). TLR3 and TLR4 mRNA expression varied significantly between control, hyperplasia and endometrial adenocarcinoma samples (Kruskal-Wallis test, P < 0.01). For both receptors, we observe a significant decrease in mRNA abundance in endometrial hyperplasia and adenocarcinoma samples, when compared to postmenopausal endometrium (P < 0.05; figure 5A–B). In undifferentiated G3 carcinoma, TLR3 and TLR4 mRNA levels were significantly lower than in postmenopausal controls (P < 0.01) and in hyperplasic endometrial tissues (P < 0.05, figure 5C–D).

Bottom Line: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens.In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls.Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Anatomy II, University of Duisburg-Essen, Essen, Germany.

ABSTRACT

Background: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens. TLRs are expressed in the human endometrium and their regulation might be crucial for the pathogenesis of endometrial diseases.

Methods: TLR3 and TLR4 expression was investigated during the menstrual cycle and in postmenopausal endometrium considering peritoneal endometriosis, hyperplasia, and endometrial adenocarcinoma specimens (grade 1 to 3). The expression studies applied quantitative RT-PCR and immunolabelling of both proteins.

Results: TLR3 and TLR4 proteins were mostly localised to the glandular and luminal epithelium. In addition, TLR4 was present on endometrial dendritic cells, monocytes and macrophages. TLR3 and TLR4 mRNA levels did not show significant changes during the menstrual cycle. In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls. Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression. Endometrial hyperplasia and adenocarcinoma revealed significantly reduced receptor levels when compared with postmenopausal controls. The lowest TLR expression levels were determined in poor differentiated carcinoma (grade 3).

Conclusion: Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.

Show MeSH
Related in: MedlinePlus