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TLR3 and TLR4 expression in healthy and diseased human endometrium.

Allhorn S, Böing C, Koch AA, Kimmig R, Gashaw I - Reprod. Biol. Endocrinol. (2008)

Bottom Line: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens.In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls.Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Anatomy II, University of Duisburg-Essen, Essen, Germany.

ABSTRACT

Background: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens. TLRs are expressed in the human endometrium and their regulation might be crucial for the pathogenesis of endometrial diseases.

Methods: TLR3 and TLR4 expression was investigated during the menstrual cycle and in postmenopausal endometrium considering peritoneal endometriosis, hyperplasia, and endometrial adenocarcinoma specimens (grade 1 to 3). The expression studies applied quantitative RT-PCR and immunolabelling of both proteins.

Results: TLR3 and TLR4 proteins were mostly localised to the glandular and luminal epithelium. In addition, TLR4 was present on endometrial dendritic cells, monocytes and macrophages. TLR3 and TLR4 mRNA levels did not show significant changes during the menstrual cycle. In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls. Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression. Endometrial hyperplasia and adenocarcinoma revealed significantly reduced receptor levels when compared with postmenopausal controls. The lowest TLR expression levels were determined in poor differentiated carcinoma (grade 3).

Conclusion: Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.

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Related in: MedlinePlus

TLR3 and TLR4 transcript are expressed in endometrium during the menstrual cycle. Columns indicate mean TLR3 and TLR4 mRNA quantities from endometrium in proliferative (n = 16), secretory (n = 11) and menstrual phase (n = 8) run in triplicates. The y-axis is scaled logarithmically; error bars represent the standard deviation of the mean.
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Figure 1: TLR3 and TLR4 transcript are expressed in endometrium during the menstrual cycle. Columns indicate mean TLR3 and TLR4 mRNA quantities from endometrium in proliferative (n = 16), secretory (n = 11) and menstrual phase (n = 8) run in triplicates. The y-axis is scaled logarithmically; error bars represent the standard deviation of the mean.

Mentions: Both receptors were expressed in all endometrial biopsies with the averaged TLR4 mRNA levels being higher (20-fold) than TLR3 (figure 1). This difference was the greatest in the shed menstrual endometrium, where TLR4 transcripts were 564-fold higher than those for TLR3. The relative abundance of both transcripts did not vary throughout the menstrual cycle (figure 1).


TLR3 and TLR4 expression in healthy and diseased human endometrium.

Allhorn S, Böing C, Koch AA, Kimmig R, Gashaw I - Reprod. Biol. Endocrinol. (2008)

TLR3 and TLR4 transcript are expressed in endometrium during the menstrual cycle. Columns indicate mean TLR3 and TLR4 mRNA quantities from endometrium in proliferative (n = 16), secretory (n = 11) and menstrual phase (n = 8) run in triplicates. The y-axis is scaled logarithmically; error bars represent the standard deviation of the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2543020&req=5

Figure 1: TLR3 and TLR4 transcript are expressed in endometrium during the menstrual cycle. Columns indicate mean TLR3 and TLR4 mRNA quantities from endometrium in proliferative (n = 16), secretory (n = 11) and menstrual phase (n = 8) run in triplicates. The y-axis is scaled logarithmically; error bars represent the standard deviation of the mean.
Mentions: Both receptors were expressed in all endometrial biopsies with the averaged TLR4 mRNA levels being higher (20-fold) than TLR3 (figure 1). This difference was the greatest in the shed menstrual endometrium, where TLR4 transcripts were 564-fold higher than those for TLR3. The relative abundance of both transcripts did not vary throughout the menstrual cycle (figure 1).

Bottom Line: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens.In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls.Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Anatomy II, University of Duisburg-Essen, Essen, Germany.

ABSTRACT

Background: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens. TLRs are expressed in the human endometrium and their regulation might be crucial for the pathogenesis of endometrial diseases.

Methods: TLR3 and TLR4 expression was investigated during the menstrual cycle and in postmenopausal endometrium considering peritoneal endometriosis, hyperplasia, and endometrial adenocarcinoma specimens (grade 1 to 3). The expression studies applied quantitative RT-PCR and immunolabelling of both proteins.

Results: TLR3 and TLR4 proteins were mostly localised to the glandular and luminal epithelium. In addition, TLR4 was present on endometrial dendritic cells, monocytes and macrophages. TLR3 and TLR4 mRNA levels did not show significant changes during the menstrual cycle. In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls. Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression. Endometrial hyperplasia and adenocarcinoma revealed significantly reduced receptor levels when compared with postmenopausal controls. The lowest TLR expression levels were determined in poor differentiated carcinoma (grade 3).

Conclusion: Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.

Show MeSH
Related in: MedlinePlus