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The effects of rivastigmine plus selegiline on brain acetylcholinesterase, (Na, K)-, Mg-ATPase activities, antioxidant status, and learning performance of aged rats.

Carageorgiou H, Sideris AC, Messari I, Liakou CI, Tsakiris S - Neuropsychiatr Dis Treat (2008)

Bottom Line: No statistically significant difference was observed in the cognitive performance.Both rivastigmine and selesiline alone improved performance, although they influenced the biochemical parameters in a different way.The combination of the two drugs did not affect learning performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Medical School, University of Athens Athens, Greece.

ABSTRACT

Unlabelled: We investigated the effects of rivastigmine (a cholinesterase inhibitor) and selegiline ((-)deprenyl, an irreversible inhibitor of monoamineoxidase-B), alone and in combination, on brain acetylcholinesterase (AChE), (Na(+), K(+))-, Mg(2+)-ATPase activities, total antioxidant status (TAS), and learning performance, after long-term drug administration in aged male rats. The possible relationship between the biochemical and behavioral parameters was evaluated.

Methods: Aged rats were treated (for 36 days) with rivastigmine (0.3 mg/kg rat/day ip), selegiline (0.25 mg/kg rat/day im), rivastigmine plus selegiline in the same doses and way of administration as separately. Aged and adult control groups received NaCl 0.9% 0.5 ml ip.

Results: TAS was lower in aged than in adult rats, rivastigmine alone does not affect TAS, decreases AChE activity, increases (Na(+), K(+))-ATPase and Mg(2+)-ATPase activity of aged rat brain and improves cognitive performance. Selegiline alone decreases free radical production and increases AChE activity and (Na(+), K(+))-ATPase activity, improving cognitive performance as well. In the combination: rivastigmine seems to cancel selegiline action on TAS and AChE activity, while it has additive effect on (Na(+), K(+))-ATPase activity. In the case of Mg(2+)-ATPase selegiline appears to attenuate rivastigmine activity. No statistically significant difference was observed in the cognitive performance.

Conclusion: Reduced TAS, AChE activity and learning performance was observed in old rats. Both rivastigmine and selesiline alone improved performance, although they influenced the biochemical parameters in a different way. The combination of the two drugs did not affect learning performance.

No MeSH data available.


Related in: MedlinePlus

Effects of rivastigmine, selegiline, and rivastigmine + selegiline on object recognition task on day 34 of the administration of the drugs. Values of the groups of aged control rats and of rivastigmine indicate the mean ± standard error (SE) of ten independent experiments (ten rats). Values of the groups of adult control rats, of selegiline and of rivastigmine + selegiline indicate the mean ± (SE) of eleven independent experiments (eleven rats).Notes: NS, nonstatistical significance; **P < 0.05; ***P < 0.001 compared with aged control group.
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fig5: Effects of rivastigmine, selegiline, and rivastigmine + selegiline on object recognition task on day 34 of the administration of the drugs. Values of the groups of aged control rats and of rivastigmine indicate the mean ± standard error (SE) of ten independent experiments (ten rats). Values of the groups of adult control rats, of selegiline and of rivastigmine + selegiline indicate the mean ± (SE) of eleven independent experiments (eleven rats).Notes: NS, nonstatistical significance; **P < 0.05; ***P < 0.001 compared with aged control group.

Mentions: In the object recognition test the discrimination between familiar and novel objects was significantly better in selegiline-treated (P < 0.001, Mann-Whitney U = 4.50) or rivastigmine-treated rats (P < 0.001, Mann-Whitney U = 0.50) than aged saline-treated rats. There was no statistically significant difference in the discrimination index between (R + S) group (Mann-Whitney U = 12.00) and aged control group (Figure 5).


The effects of rivastigmine plus selegiline on brain acetylcholinesterase, (Na, K)-, Mg-ATPase activities, antioxidant status, and learning performance of aged rats.

Carageorgiou H, Sideris AC, Messari I, Liakou CI, Tsakiris S - Neuropsychiatr Dis Treat (2008)

Effects of rivastigmine, selegiline, and rivastigmine + selegiline on object recognition task on day 34 of the administration of the drugs. Values of the groups of aged control rats and of rivastigmine indicate the mean ± standard error (SE) of ten independent experiments (ten rats). Values of the groups of adult control rats, of selegiline and of rivastigmine + selegiline indicate the mean ± (SE) of eleven independent experiments (eleven rats).Notes: NS, nonstatistical significance; **P < 0.05; ***P < 0.001 compared with aged control group.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2536534&req=5

fig5: Effects of rivastigmine, selegiline, and rivastigmine + selegiline on object recognition task on day 34 of the administration of the drugs. Values of the groups of aged control rats and of rivastigmine indicate the mean ± standard error (SE) of ten independent experiments (ten rats). Values of the groups of adult control rats, of selegiline and of rivastigmine + selegiline indicate the mean ± (SE) of eleven independent experiments (eleven rats).Notes: NS, nonstatistical significance; **P < 0.05; ***P < 0.001 compared with aged control group.
Mentions: In the object recognition test the discrimination between familiar and novel objects was significantly better in selegiline-treated (P < 0.001, Mann-Whitney U = 4.50) or rivastigmine-treated rats (P < 0.001, Mann-Whitney U = 0.50) than aged saline-treated rats. There was no statistically significant difference in the discrimination index between (R + S) group (Mann-Whitney U = 12.00) and aged control group (Figure 5).

Bottom Line: No statistically significant difference was observed in the cognitive performance.Both rivastigmine and selesiline alone improved performance, although they influenced the biochemical parameters in a different way.The combination of the two drugs did not affect learning performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Medical School, University of Athens Athens, Greece.

ABSTRACT

Unlabelled: We investigated the effects of rivastigmine (a cholinesterase inhibitor) and selegiline ((-)deprenyl, an irreversible inhibitor of monoamineoxidase-B), alone and in combination, on brain acetylcholinesterase (AChE), (Na(+), K(+))-, Mg(2+)-ATPase activities, total antioxidant status (TAS), and learning performance, after long-term drug administration in aged male rats. The possible relationship between the biochemical and behavioral parameters was evaluated.

Methods: Aged rats were treated (for 36 days) with rivastigmine (0.3 mg/kg rat/day ip), selegiline (0.25 mg/kg rat/day im), rivastigmine plus selegiline in the same doses and way of administration as separately. Aged and adult control groups received NaCl 0.9% 0.5 ml ip.

Results: TAS was lower in aged than in adult rats, rivastigmine alone does not affect TAS, decreases AChE activity, increases (Na(+), K(+))-ATPase and Mg(2+)-ATPase activity of aged rat brain and improves cognitive performance. Selegiline alone decreases free radical production and increases AChE activity and (Na(+), K(+))-ATPase activity, improving cognitive performance as well. In the combination: rivastigmine seems to cancel selegiline action on TAS and AChE activity, while it has additive effect on (Na(+), K(+))-ATPase activity. In the case of Mg(2+)-ATPase selegiline appears to attenuate rivastigmine activity. No statistically significant difference was observed in the cognitive performance.

Conclusion: Reduced TAS, AChE activity and learning performance was observed in old rats. Both rivastigmine and selesiline alone improved performance, although they influenced the biochemical parameters in a different way. The combination of the two drugs did not affect learning performance.

No MeSH data available.


Related in: MedlinePlus