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Suppressive Effects of Selected Food Phytochemicals on CD74 Expression in NCI-N87 Gastric Carcinoma Cells.

Sekiguchi H, Washida K, Murakami A - J Clin Biochem Nutr (2008)

Bottom Line: NCI-N87 cells were treated separately with 25 different food phytochemicals (4-100 microM) for 48 h and subjected to our novel assay.From those results, a citrus coumarin, bergamottin, was indicated to be the most promising compound with an LC(50)/IC(50) value greater than 7.1, followed by luteolin (>5.4), nobiletin (>5.3), and quercetin (>5.1).Our findings suggest that these CD74 suppressants are unique candidates for preventing H. pylori adhesion and subsequent infection with reasonable action mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

ABSTRACT
Helicobacter pylori (H. pylori) is one of the most widespread human pathogens, and plays major roles in chronic gastritis and gastric cancer. CD74 of gastric epithelial cells has recently been identified as an adhesion molecule to urease in H. pylori. In this study, we found that CD74 is highly expressed in a constitutive manner in NCI-N87 human gastric carcinoma cells at both the protein and mRNA levels as compared with Hs738St./Int fetal gastric cells. Subsequently, a novel cell-based ELISA able to rapidly screen the suppressive agents of CD74 expression was established. NCI-N87 cells were treated separately with 25 different food phytochemicals (4-100 microM) for 48 h and subjected to our novel assay. From those results, a citrus coumarin, bergamottin, was indicated to be the most promising compound with an LC(50)/IC(50) value greater than 7.1, followed by luteolin (>5.4), nobiletin (>5.3), and quercetin (>5.1). Our findings suggest that these CD74 suppressants are unique candidates for preventing H. pylori adhesion and subsequent infection with reasonable action mechanisms.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of selected food factors.
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Related In: Results  -  Collection


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Figure 2: Chemical structures of selected food factors.

Mentions: A total of 25 food factors (Fig. 2) were selected based on their previously reported anti-inflammatory in vitro and in vivo activities [17, 20–22]. Each was subjected to our novel cell-based ELISA established in the present study. As shown in Table 1, 65% of the tested compounds exhibited strong cytotoxicity [cell viability (CV) >70%] at a concentration of 100 µM after 48 h. Subsequently, some of the compounds, such as curcumin, ACA, and ursolic acid, continued to induce cytotoxicity (20 µM, CVs = 12%, 20%, and 38%, respectively). Notably, silymarin (100 µM) and bergamottin (20 µM) markedly inhibited CD74 expression [inhibitory rates (IRs) = 91% and 74%, respectively) without considerable cytotoxicity. Further, (−)-catechin, gallic acid, zerumbone, auraptene, nobiletin, genistein, quercetin, and luteolin (20 µM) were moderately suppressive (IRs = 40–65%), while none of the compounds showed marked suppression at a concentration of 20 µM. As summarized in Table 2, the IC50 values for zerumbone, auraptene, nobiletin, quercetin, luteolin, and bergamottin were lower (IC50 = 14–20 µM) than for other compounds. Marginal cytotoxicity was observed with some compounds, including (−)-catechin and gallic acid. Based on the LC50/IC50 ratios, we identified bergamottin as the most promising CD74 suppressant (LC50/IC50 >7.1), followed by in order by luteolin, nobiletin, and quercetin (LC50/IC50 >5.4, >5.3, and >5.1, respectively).


Suppressive Effects of Selected Food Phytochemicals on CD74 Expression in NCI-N87 Gastric Carcinoma Cells.

Sekiguchi H, Washida K, Murakami A - J Clin Biochem Nutr (2008)

Chemical structures of selected food factors.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2533715&req=5

Figure 2: Chemical structures of selected food factors.
Mentions: A total of 25 food factors (Fig. 2) were selected based on their previously reported anti-inflammatory in vitro and in vivo activities [17, 20–22]. Each was subjected to our novel cell-based ELISA established in the present study. As shown in Table 1, 65% of the tested compounds exhibited strong cytotoxicity [cell viability (CV) >70%] at a concentration of 100 µM after 48 h. Subsequently, some of the compounds, such as curcumin, ACA, and ursolic acid, continued to induce cytotoxicity (20 µM, CVs = 12%, 20%, and 38%, respectively). Notably, silymarin (100 µM) and bergamottin (20 µM) markedly inhibited CD74 expression [inhibitory rates (IRs) = 91% and 74%, respectively) without considerable cytotoxicity. Further, (−)-catechin, gallic acid, zerumbone, auraptene, nobiletin, genistein, quercetin, and luteolin (20 µM) were moderately suppressive (IRs = 40–65%), while none of the compounds showed marked suppression at a concentration of 20 µM. As summarized in Table 2, the IC50 values for zerumbone, auraptene, nobiletin, quercetin, luteolin, and bergamottin were lower (IC50 = 14–20 µM) than for other compounds. Marginal cytotoxicity was observed with some compounds, including (−)-catechin and gallic acid. Based on the LC50/IC50 ratios, we identified bergamottin as the most promising CD74 suppressant (LC50/IC50 >7.1), followed by in order by luteolin, nobiletin, and quercetin (LC50/IC50 >5.4, >5.3, and >5.1, respectively).

Bottom Line: NCI-N87 cells were treated separately with 25 different food phytochemicals (4-100 microM) for 48 h and subjected to our novel assay.From those results, a citrus coumarin, bergamottin, was indicated to be the most promising compound with an LC(50)/IC(50) value greater than 7.1, followed by luteolin (>5.4), nobiletin (>5.3), and quercetin (>5.1).Our findings suggest that these CD74 suppressants are unique candidates for preventing H. pylori adhesion and subsequent infection with reasonable action mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

ABSTRACT
Helicobacter pylori (H. pylori) is one of the most widespread human pathogens, and plays major roles in chronic gastritis and gastric cancer. CD74 of gastric epithelial cells has recently been identified as an adhesion molecule to urease in H. pylori. In this study, we found that CD74 is highly expressed in a constitutive manner in NCI-N87 human gastric carcinoma cells at both the protein and mRNA levels as compared with Hs738St./Int fetal gastric cells. Subsequently, a novel cell-based ELISA able to rapidly screen the suppressive agents of CD74 expression was established. NCI-N87 cells were treated separately with 25 different food phytochemicals (4-100 microM) for 48 h and subjected to our novel assay. From those results, a citrus coumarin, bergamottin, was indicated to be the most promising compound with an LC(50)/IC(50) value greater than 7.1, followed by luteolin (>5.4), nobiletin (>5.3), and quercetin (>5.1). Our findings suggest that these CD74 suppressants are unique candidates for preventing H. pylori adhesion and subsequent infection with reasonable action mechanisms.

No MeSH data available.


Related in: MedlinePlus